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redox indicator
changes colour when a substance is reduced or oxidised
DCPIP and methyl blue
redox indicators used to monitor respiration in yeast cells without damaging them
colour change
blue to colourless
reduction
taking up of H+ ions causes colour change
rate of colour change
directly proportional to rate of respiration
rate of respiration
inversely proportional to time
investigating temperature
using a waterbath to change temperature and measure the time taken for colour change
investigating concentration
measure the time taken for colour change at different glucose concentrations
control variables
volume of dye and yeast…type and concentration of substrate