Fixation

For mnemonics, refer to other flashcard

Best general tissue fixative

10% NBF

Most widely used for EM

Glutaraldehyde

Glutaraldehyde has _ reactive aldehyde groups separated by _ carbon atoms

2 reactive aldehyde groups

3 carbon atoms

Karnovsky’s is for what microscopy

Electron microscopy

Aldehyde fixative associated with:

• fixing lipids for frozen section

Formol-calcium

Aldehyde fixative associated with:

• CNS fixative

10% formol-saline

Acrolein is a mixture with

Glutaraldehyde / Formaldehyde

Most commonly used metallic fixative (in general)

Mercuric chloride fixative

Mercuric chloride fixative associated with:

• stool preservative

Schaudinn’s

Mercuric chloride fixative associated with:

• recommended for trichrome staining

Zenker’s

Mercuric chloride fixative associated with:

• for fixing pituitary gland, BM, and blood-containing organs

Helly’s

Helly’s is AKA

Zenker-formol

Zenker-formol is AKA

Helly’s

Osmium tetroxide fixative associated with:

• Permanently fixes fats for EM

• note: dont get confused w/ Formol-calcium (for frozen section to)

Flemming’s with acetic acid

Osmium tetroxide fixative associated with:

• fixing mitochrondria

Flemming’s without acetic acid

Chromate fixative associated with:

• carbohydrate preservation

Chromic acid

Chromate fixative associated with:

• Rickettsia

• Necrotic tissue

Orth’s

Chromate fixative associated with:

• fixing mitochrondria

• note: dont get confused with Flemming’s without acetic acid (Osmium tetroxide to)

Potassium dichromate

Alcoholic fixative associated with:

• preserves but does not fix glycogen

• causes polarization

Ethanol

Alcoholic fixative associated with:

• touch preparation

Isopropanol

Alcoholic fixative associated with:

• for fixing chromosomes

• 1-3 hrs

Carnoy’s

Alcoholic fixative associated with:

• mucopolysachharides

Newcomer’s

Alcoholic fixative associated with:

• fixing sputum

Alcoholic formalin

Alcoholic formalin is AKA

Gendre’s

Gendre’s is AKA

Alcoholic formalin