Producing DNA Fragments

what does recombinant DNA tech involve

transferring fragments of DNA from one organism or species to another

what does recombinant DNA tech result in

translation within recipient

why is recombinant DNA tech possible

universal nature of dna

transcription and DNA universal

3 ways to produce DNA fragments

conversion of mRNA into cDNA using reverse transcriptase

using endonuclease enzymes

gene machine

what does conversion of mRNA into cDNA using reverse transcriptase involve

mRNA isolated from cells

RT and free nucleotides added

RT produces cDNA (complementary DNA) using mRNA as template

what does using restriction endonuclease enzymes involve

restriction endonucleases specific to palindromic sequence and cut DNA at these sequences

each RE has a active site that is complementary to specific palindromic sequence

RE can remove DNA fragment by hydrolysis if recognition sequences are either side of it

what is the recognition sequence

palindromic sequence specific to that RE

what is a palindromic sequence

base pairs that read the same in opposite directions

what are sticky ends

unpaired bases

how are sticky ends used

can be used to bind DNA fragment to another DNA strand that has sticky ends with comp bases

what happens in the gene machine

base sequence for DNA fragment is designed

first nucleotide fixed to a support

nucleotides added in correct order

what is produced in gene machine that then needs to be edited

oligonucleotides- short section of DNA

joined together by condensation reaction to produce longer DNA fragments