Cell Fractionation and Chloroplast Isolation

These flashcards cover fundamental vocabulary and concepts related to cell fractionation, chloroplast isolation, and the associated laboratory techniques.

Cell Fractionation

A technique to separate cell components while keeping them intact.

Centrifugation

A process used in cell fractionation to separate organelles based on size and shape.

Chloroplasts

Subcellular organelles in plant cells where photosynthesis takes place.

Photosynthesis

The process of converting light energy into chemical energy.

Chlorophyll

Pigments within chloroplasts that reflect green light and are essential for photosynthesis.

Thylakoids

Flattened disc-shaped structures within chloroplasts that contain chlorophyll.

Stromal Lamella

Structures that connect thylakoids in chloroplasts.

Hemocytometer

A device used to determine the concentration of cells in a sample.

Spectrophotometry

A method used to measure the amount of chlorophyll a in the extract.

Differential Centrifugation

A technique to separate cell components based on their size and density.

Green Algae

Aquatic plants that, like higher plants, contain chlorophyll and perform photosynthesis.

Accessory Pigments

Pigments like chlorophyll b and carotenoids that assist chlorophyll a in capturing light energy.

Light-dependent Reactions

Reactions in photosynthesis that convert light energy into chemical energy, involving chlorophyll.

Plant Cell Structure

Includes components such as cell wall, plasma membrane, nucleus, mitochondria, and chloroplasts.

Lab Worksheet

A document to be submitted at the end of the lab, summarizing the findings.

Concentration of Chlorophyll a

The amount of chlorophyll a present per chloroplast, determined through lab techniques.

Filtration

A process used during cell fractionation to remove large debris and unbroken cells.

Cytoplasm

The fluid within a cell where organelles are suspended.

Optical Density

A measure of how much light is absorbed by a solution, often used in spectrophotometry.

Lab TA Help Sessions

Optional sessions where teaching assistants provide additional assistance to students.

Exam 1

An assessment covering lab work from labs 1-6, scheduled between March 9th-13th.