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what are amino acids
building blocks of proteins (proteinogenic in human nutrition)
what are BCAAs
Branched Chain Amino Acids (valine, leucine, isoleucine), most abundant in complete protein, primarily used by muscles for energy, and promote muscle protein synthesis
how many amino acids are made into human protein
20
what are essential amino acids
9AAs that must come from diet: phenylalanine, valine, threonine, tryptophan, isoleucine, methioine, histidine, leucine, and lysine (PVT TIM HLL)
what are conditionally essential amino acids
6 AAs needed in certain conditions: arginine, cysteine, glutamine, glycine, proline, tyrosine
what starts protein digestion
chewing and HCL in stomach
what does HCL do to proteins
unfolds them into long polypeptides and activates pepsin
what is pepsin
stomach enzyme that breaks down protein
where is most protein digestion completed
small intestine, with pancreatic enzymes -> ~90% amino acids absorbed
how are AAs transported after absorption
through the portal vein to the liver
what can proteins be used for besides building tissue
catabolized for energy or used to make glucose
in low energy availability, what tissue is sacrificed for energy
skeletal muscle
what must happen before AAs enter energy pathways
deamination (removal of nitrogen groups)
where can AAs enter for energy production
different entry points of the Krebs cycle
what is protein turnover
constant breakdown and rebuilding of proteins
when is turnover highest
during stress or exercise
main protein functions
enzymes, antibodies, pumps/regulators, fluid balance, acid-base balance, and movement
what is the most abundant protein in the body
collagen
what proteins are responsible for movement
motor proteins
where is the code for protein synthesis stored
DNA -> transcribes to mRNA -> translated at ribosomes
what must be present for protein synthesis to complete
all amino acids
if an AA is missing, what happens
skeletal muscle is broken down to supply it
what is the AMDR for protein
10-35% of daily calories
general protein intake guidelines
0.8 g/kg body weight (athletes: 1.2 g/kg+)
what is more accurate: % calories or g/kg
g/kg body weight
what are the highest quality proteins
animal products and soybeans protein isolate
what is whey protein
fast-digesting, high in BCAAs
what is casein protein
slower digesting, similar to whey
what is egg protein
high quality, good for those avoiding dairy
what determines protein quality
amount of protein and digestibility/absorption
functions of protein in athletes
build muscle, promote adaptations, preserve lean mass, strengthen non-muscle tissues
athlete protein intake guidelines
1.2-2.0 g/kg (up to 3 g/kg for physique athletes)
180 lb athlete protein needs
90.9 kg -> 109-182 g protein/day
best way to distribute protein
spread throughout day (0.25-0.3 g/kg per meal), avoid backloading
what increases protein synthesis
exercise novelty, hormones post-workout, injury/illness recovery, positive energy balance
what amino acid triggers muscle protein synthesis (MPS)
700-3,000 mg per meal (with ~10 g essential amino acids)
what are optimal protein sources for MPS
whey, dairy, and other complete proteins
where does fat digestion begin
in the mouth (minor role, lingual lipase)
which organs release enzymes to digest fats
gallbladder (bile salts) and pancreas (lipases)
where are most free fatty acids (FFA) absorbed
small intestine
after absorption, how are fats transported
repackaged into chylomicrons (protein transport vehicles)
can all macronutrients be stored as fat
yes
which enzymes breaks down triglycerides for storage
lipoprotein lipase
which fatty acids must come from the diet (essential)
linoleic acid (omega-6) and alpha-linolenic acid (omega-3)
does everyone have large fat stores
yes
when is fat use highest during exercise
low-intensity exercise
what are other roles of fat
vitamin absorption, hormone regulation, cell membranes, myelin
what does hormone-sensitive lipase (HSL) do
breaks down triglycerides into glycerol and fatty acids
what are the end product of triglyceride breakdown
glycerol and 3 fatty acids
what is the body's main storage form of fat
triglycerides
energy from 1g of fat
9 kcal
saturated fatty acids
solid, no double bonds, ex: butter
unsaturated fatty acids
liquid, double bonds, ex: olive oil
short fatty acid chain length
<6
medium fatty acid chain length
8-12
long fatty acid chain length
>12
two configurations of unsaturated fatty acids
cis and trans
mon common sterol in the body
cholesterol
where is cholesterol found in food
animal products only
which cholesterol is "bad"
LDL (low density lipoprotein)
which cholesterol is "good"
HDL (high density lipoprotein)
where are saturated fats found
meat, cheese, butter
do all saturated fats affect cholesterol the same way
no, effects differ by type
what substitution is suggested to reduce CVD risk
replace saturated fats with polyunsaturated fats
health effect of monounsaturated fats
may decrease LDL and increase HDL
which polyunsaturated fats are essential
omega-6 and omega-3
role of omega-6
epithelial cell function, and gene regulation
role of omega-3 (EPA and DHA)
decrease inflammation, improve vessel function, increase HDL, and decrease triglycerides
how can EPA help athletes
may delay inflammation post-exercise, reduce joint pain, and aid recovery
DHA's role in the brain
improves cell membrane fluidity, decrease inflammation, and decrease blood flow
omega-3s and concussions
may protect against structural damage, improve recovery
how are trans fats made
hydrogenation of unsaturated fats
are trans fats healthy
no (artificial ones increase CVD risk)
are natural trans fats (meat, dairy) as harmful
not significantly in small amounts
what % of daily calories should come from fat (AMDR)
20-35%
how much should come from saturated fats
<2% of total calories
recommended intake of linoleic acid
4.4-13 g/day
recommended intake of alpha-linolenic acid
0.5-1.6 g/day
recommended intake of ecisapentaenoic acid
250 mg/day
recommended intake of docosahexaenoic acid
250 mg/day
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where does carbohydrate digestion occur
mouth (chewing, salivary amylase), stomach, and small intestine villi
why must carbs be broken down into monosaccharides
only monosaccharides can enter the bloodstream
what does a higher glycemic index food mean
causes a higher, faster blood glucose response
how does exercise intensity affect carb use
harder exercise -> greater carb reliance
why do athletes need adequate carb stores
to perform near max intensity exercise
what are 3 primary causes of muscle fatigue
1. muscles can't produce enough force
2. high ATP demand -> incomplete glucose oxidation + acidity buildup
3. muscle energy reserves depleted
how much CHO does the brain consume daily
~120 g/day (~20% of RMR)
what can hypoglycemia or impaired glucose regulation cause
lack of sharpness, mood disorders, possible link to "type 3 diabetes"
what is the AMDR for carbs
45-65% of total energy intake (TEI)
what is the RDA for carbs
130 g/day minimum (for brain function)
carbohydrate needs for pregnancy and lactation
pregnant = 175 g/day; breastfeeding = 210 g/day
what is the max added sugar intake recommendation
<10% of total carbs
daily fiber recommendations
- men: 38 g/day
- women: 25 g/day
- pregnant/lactating: 28-29 g/day
what is the average US fiber intake
~15 g/day
what must accompany increased fiber intake
adequate hydration
health benefits of fiber
binds, toxins, improves elimination, lower cancer risk, increases stool bulk, and binds cholesterol for removal
what are nutritive sweeteners and their risks
provide calories but little nutrition -> overconsumption can cause dyslipidemia, inflammation, diabetes, and obesity
why is high-fructose corn syrup used
cheap, long shelf life, makes food palatable; ~45-55% fructose
what are non-nutritive sweeteners
saccharin, aspartame, ace-k, sucralose, neotame, advantame, stevia, and leu han guo (adds sweetness without calories)