bio 1AL- lab 8 bioinformatics

complementation group

strains that fail to complement (they are mutated in the same gene)

• all streaks which fail to grow are in one complementation group

• streaks that grow could be one group but we don’t know unless they are crossed

cDNA

complementary DNA- it is complementary to the mRNA, used during sequencing

reverse transcription

process of getting cDNA from mRNA

• carried out by reverse transcriptase

finding a reading frame

must start with start codon, and not have a stop codon for a long time

• start codon: AUG

missense mutation

point mutation, changes type of protein expressed

nonsense mutation

introduces stop codon (UAG)

frameshift mutation

inserts an extra letter, causing everything downstream to change

silent mutation

point mutation that keeps the same protein expressed