Micro Practical 2

Amoeba classifications?

Domain Eukarya, kingdom Protozoa, phylum Amoebozoa, Amoeba proteus

Trypanosoma classifications

Domain Eukarya, kingdom Protozoa, phylum Euglenozoans, Trypanosoma

Euglena classifications

Domain Eukarya, kingdom Protozoa, phylum Euglenozoans, Euglena

Alveolates classifications

Domain Eukarya, kingdom Protozoa, phylum Alveolates, Paramecium

Cyanobacteria classifcations

Domain Bacteria, group Cyanobacteria, group Anabaena

Zygomycota classifications

Fungi, phylum Zygomycota, Rhizopus

Phylum Ascomycota genuses

Saccharomyces cerevisiae, Sordaria, Morchella, Lichens, Penicillium

Phylum Basidiomycota genus

Mushroom

what organisms form lichens?

fungi and algae or fungi and cyanobacteria

what stain is used on molds?

lactophenol cotton blue

what is a plaque?

area where cells lysed and form clear areas

how to calculate PFU/mL

number of plaques divided by dilution plated

what is a countable plate?

25-250

what organisms was used to form the biofilm?

Staphylococcus epidermidis

What stain used to stain the biofilm?

methylene blue

materials to establish the biofilm

coplin jar, incubate slides in jar

Whats the ability of bacteria to communicate and coordinate behavior is called?

quorum sensing

How to calculate chi square?

(observed - expected)^2 / expected, then add all together

How to accept or reject a null hypothesis?

x²>critical value: reject

x²<critical value: accept

How to find critical value?

go to degrees of freedom and 0.05 probability

how to find degrees of freedom?

numbers of categories minus 1

Amoeba, pseudopodia

Amoeba, pseudopodia

Euglena

Trypanosoma, flagella

Paramecium, cilia

Paramecium

Anabaena, heterocyst

Rhizopus, sporangium, sporangiophore

Rhizopus, zygospore

Saccharomyces cerevisiae, budding

Sordaria, perithecium, ascus

Morchella, morel, pileus, stipe

Penicillium, conidiospores, phialides, conidiophores

Mushroom, pileus, lamella, stipe

Lichens, crustose, foliose, fruticose