Microbiology Lab 5 - Negative Staining Midterm Practice

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10 Terms

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What is stained in a negative stain?

Background, not the cell

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Common dyes used?

Nigrosin or India Ink

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Why is Nigrosin useful?

Large colloidal particles can’t enter cell → clear outline.

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What pH makes Nigrosin negatively charged?

pH 7

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Is heat used in negative staining?

No, avoids distortion

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Best bacteria type to view with this method?

Coccobacilli or short rods.

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What kind of stain is it (simple or differential)?

Simple

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Chromophore charge in acidic stain?

Negative

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Why does background become dark?

Acidic dye repelled by cell → stains background.

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Main benefit of no heat-fixing?

Bacteria stay alive shape-wise; less shrinking.