Practice quiz on molecular biology techniques

When introduced into living cells, antisense RNA molecules bind

to complementary mRNA sequences and block protein synthesis.

Match the detection method to the correct description: Northern blotting

DNA probe to detect cellular mRNA target

Match the detection method to the correct description: Southern blotting

DNA probe to detect genomic DNA target

Match the detection method to the correct description: Western blotting

Antibody probe to detect a protein target

Match the detection method to the correct description: Microarray

cDNA probe to detect levels of cellular mRNA expresses=d

Nucleic acid hybridization is the 

formation of a double-stranded molecule from the interaction of two complementary-sequence single-stranded molecules.

Which of these features are required to select recombinant bacteria?

a plasmid with an antibiotic resistance gene

When putting an insert into a plasmid

the insert and plasmid have to have compatible ends

On an agarose gel  to separate DNA fragments,

the largest DNA fragments migrate the slowest

Match the type of plasmid with the use for the plasmid.: cloning plasmid

make large amounts of DNA

Match the type of plasmid with the use for the plasmid.: expression plasmid

make large amounts of protein

Match the type of plasmid with the use for the plasmid.: reporter plasmid

make protein when gene regulation allows it

In performing PCR there are several steps. Match these steps to the process involved.: denaturation

separating all double stranded DNA

In performing PCR there are several steps. Match these steps to the process involved.: annealing

primers attach to the denatured DNA strands

In performing PCR there are several steps. Match these steps to the process involved.: extension

Taq polymerase replicates the DNA

Match the technique to the description.: stable transfection

insert DNA is integrated into the genome

Match the technique to the description.: transient transfection

plasmid DNA is expressed in eukaryotes

Match the technique to the description.: transformation

plasmid DNA is replicated and expressed in bacteria

Match the technique to the description.: gene editing

CRISPR/Cas technology

SDS-polyacrylamide gel electrophoresis (SDS-PAGE) separates proteins by

size

The first enzymatic step in making a cDNA from mRNA uses

reverse transcriptase

Restriction endonucleases are enzymes that

cleave DNA only at specific sequences

The CRISPR/Cas system has revolutionized the efficiency with which scientists can mutate a gene in vivo to investigate the functional role of the protein it encodes. Which component is not essential in the CRISPR/Cas system?

Access to target mRNA

Transgenic mice are usually produced by

injecting DNA containing the gene into the pronucleus of a fertilized egg.

The most common method of DNA sequencing is based on premature termination of DNA synthesis by inclusion of

dideoxynucleotides

Taq polymerase is used in the polymerase chain reaction because it

is stable at high temperatures