2.7 Fluid Thioglycollate Broth

Describe the composition of fluid thioglycollate broth (FTB)

Composed of different supplements depending on the specific needs of the organism, key things include:

1. Yeast extract

2. Pancreatic digest

3. Sodium thioglycollate

4. L-cystine

5. Rasazurin

What purpose is FTB used for?

Best medium for a wide variety of aerobic and anaerobic, complex (needs specific nutrient requirements), and non-complex bacteria

Why does the Fluid thioglycollate broth contain agar?

Thick agar helps to decrease the amount of oxygen that diffuses to the bottom

Explain the purpose of resazurin

An indicator of oxygen that turns pink in the presence of oxygen or remains colorless in the absence of oxygen

Where would you expect to see growth of a strict aerobe?

At the top of the FTB tube due to the requirement of oxygen to grow

Where would you expect to see growth of a anaerobe?

At the bottom of the FTB tube due to no oxygen present

Where would you expect to see growth of a aerotolerant anaerobe?

All throughout the tube evenly because it grows without the need for oxygen, and only in anaerobe conditions

Why are aerotolerant anaerobes able to grow in the presence of oxygen?

Contain SOD enzyme that neutralizes harmful forms of oxygen

Where would you expect to see growth of a facultative anaerobe?the

Growth is the best when oxygen is present, but occurs throughout the tube

Where would you expect to see growth of a microaerophiles?

At the center of the FTB tube, where growth occurs at low concentrations of oxygen (only aerobic)

Which categories of aerotolerance bacteria contain SOD or catalase?

Strict aerobes, facultative anaerobes, Microphiles, Aerotolerant anaerobes

Describe the action of SOD and catalase

SOD converts reactive oxygen into hydrogen peroxide

Catalase and Peroxidase converts hydrogen peroxide into water and oxygen (non-reactive)

Explain the procedure of 2.7 FTB

1. Properly label tubes (one without inoculation)

2. Inoculate each tube with broths using a flamed inoculating loop

3. Incubate