AUBF 11: CLASSIC CHEMICAL TESTS OF URINE

heat & acetic acid test modified heat & acetic acid test heat coagulation method Purdy's test Osgood-Haskin test

5 protein qualitative tests using heat & acid

heat & acetic acid test

• protein qualitative test using heat & acid

• Bauer, et al

⚗️diluted acetic acid + heat

modified heat & acetic acid test

• protein qualitative test using heat & acid

⚗️saturated NaCl sol'n + acetic acid + heat

heat coagulation method

• protein qualitative test using heat & acid

• Henry, 196

⚗️acetic acid & Na citrate + heat

Purdy's test

• protein qualitative test using heat & acid

• modification of heat & acetic acid

⚗️saturated aqueous NaCl sol'n & acetic acid + heat

Osgood-Haskin test

• protein qualitative test using heat & acid

⚗️ 50% acetic acid ⚗️ saturated NaCl

sulfosalicylic acid test Heller's ring test picric acid test Robert's ring test K ferricyanide test Spiegler's test

6 qualitative tests for protein using acid only

Exton's sulfosalicylic acid (SSA) test

• qualitative test for protein using acid only

⚗️sulfosalicylic acid ⚗️Na sulfate anhydrous ⚗️ dH2O

➕ white opaque ring at zone of contact

Heller's ring test

• qualitative test for protein using acid only

⚗️conc. nitric acid

➕ white opaque ring at zone of contact

picric acid test

• qualitative test for protein using acid only

⚗️saturated picric acid

➕ white opaque ring at zone of contact

Robert's ring test

• qualitative test for protein using acid only

⚗️saturated aqueous Mg sulfate ⚗️ conc. nitric acid

➕ white opaque ring at zone of contact

K ferricyanide test

• qualitative test for protein using acid only

⚗️glacial acetic acid ⚗️K ferrocyanide

➕ white opaque ring at zone of contact

Spiegler's test

• qualitative test for protein using acid only

⚗️mercuric chloride ⚗️succinic acid ⚗️dH2O

➕ white opaque ring at zone of contact

Esbach's test Tsuchiya's modification Shevky & Stafford's method Kingsbury's test Kingsbury-Clark method Kwilecki's test Purdy's method

7 quantitative tests for protein

Esbach's test

• quantitative test for protein

⚗️picric acid ⚗️citric acid ⚗️ dH2O

➕ measure height of ppt

Tsuchiya's modification

• quantitative test for protein

⚗️phosphotungstic acid ⚗️95% alcohol ⚗️conc. HCl

➕ measure height of ppt

Shevky & Stafford's method

• quantitative test for protein

⚗️Tsuchiya's reagent & centrifugation

➕ measure height of ppt

Kingsbury's test

• quantitative test for protein

⚗️ 3% SSA

➕ measure height of ppt

Kingsbury-Clark method

• quantitative test for protein

⚗️ 3% SSA

➕ measure density of ppt

Kwilecki's test

• quantitative test for protein

• modification of Esbach's

⚗️10% ferric chloride ⚗️Esbach's reagents

➕ measure volume of ppt

Purdy's method

• quantitative test for protein

⚗️acetic acid ⚗️ 10% K ferrocyanide

➕ measure volume of ppt

heat & acetic acid test Bradshaw test Putnam, et al. test

3 chemical tests for Bence Jones protein

heat & acetic acid test

• chemical test for Bence Jones protein

⚗️acetic acid + heat (100°C)

Bradshaw test

• chemical test for Bence Jones protein

⚗️Heller's test (nitric acid) / SSA + concentrated HCl

Putnam, et al. test

• chemical test for Bence Jones protein

⚗️2M acetate buffer ⚗️glacial acetic acid with water

heat & acetic acid test Hepler's test mucicarmine stain

3 chemical tests for mucin

heat & acetic acid test

chemical test for mucin

⚗️ ➕

Hepler's test

chemical test for mucin

⚗️ ➕

mucicarmine stain

chemical test for mucin

⚗️ ➕

Benedict's qualitative test, Haine's test, Trommer's test, Nylander's test, Moore-Heller's test fermentation tests phenylhydrazine test thin layer chromatography Fehling's method

5 chemical tests for glucose

Benedict's qualitative test Haine's test Trommer's test Nylander's test Moore-Heller's test

chemical test for glucose

⚗️ ➕

fermentation tests

chemical test for glucose

⚗️ ➕

phenylhydrazine test

chemical test for glucose

⚗️ ➕

thin layer chromatography

chemical test for glucose

⚗️ ➕

Fehling's method

chemical test for glucose

⚗️ ➕

⭐️ Seliwanoff's test Borchardt's test Barfoed's test

3 chemical tests for fructose

Seliwanoff's test

⭐️ chemical test for fructose

⚗️ ➕

Borchardt's test

chemical test for fructose

⚗️ ➕

Barfoed's test

chemical test for fructose

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⭐️ Bial's test (Orcinol) Tauber's test Tollen's test Cole's test aniline test

5 chemical tests for pentose

Bial's test (Orcinol)

⭐️ chemical test for pentose

⚗️ ➕

Tauber's test

chemical test for pentose

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Tollen's test

chemical test for pentose

⚗️ ➕

Cole's test

chemical test for pentose

⚗️ ➕

aniline test

chemical test for pentose

⚗️ ➕

⭐️ Rubner's test Ornsby & Johnson test Woehlk's test nitric acid test phenylhhydrazine test thin layer chromatography

6 chemical tests for lactose

Rubner's test

⭐️ chemical test for lactose

⚗️ ➕

Ornsby & Johnson test

chemical test for lactose & maltose

⚗️ ➕

Woehlk's test

chemical test for lactose

⚗️ ➕

nitric acid test

chemical test for lactose

⚗️ ➕

phenylhhydrazine test

chemical test for lactose

⚗️ ➕

thin layer chromatography

chemical test for lactose

⚗️ ➕

⭐️ Barfoed's test Ormsby & Johnson test thin layer chromatography

3 chemical tests for maltose

Barfoed's test

⚗️ ➕

thin layer chromatography

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Rothera's test Lange test acetone test Rantzmann Legal's test Jackson-Taylor Trommer test

chemical tests for acetoacetate & acetone that use Na nitroprusside resulting to yellow color

Rothera's test

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Lange test

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acetone test

⚗️ ➕

Rantzmann

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Legal's test

⚗️ ➕

Jackson-Taylor

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Trommer test

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Lieben's iodoform test Gunning's test

chemical tests for acetoacetate & acetone that use iodoform resulting to yellow color

⚗️ ➕

Lieben's iodoform test

⚗️ ➕

Gunning's test

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benzidine test orthrotoluidine test Guaiac's test Teichmann's test Rous test

classical chemical tests for blood

• same principle color result with that of reagent strip

benzidine test

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orthrotoluidine test

⚗️ ➕

Guaiac's test

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Teichmann's test

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Rous test

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Icto test Fouchet's test Harison's spot test Watson oxidation test Smith's test Gmelin's test nitric acid test Maher's test Rosenbach's test Ultzmann's test methylene blue test

chemical tests for bilirubin

Icto test

⚗️ ➕

Fouchet's test

⚗️ ➕

Harison's spot test

⚗️ ➕

Watson oxidation test

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Smith's test

⚗️ ➕

Gmelin's test nitric acid test

⚗️ ➕

Maher's test

⚗️ ➕

Rosenbach's test

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Ultzmann's test

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methylene blue test

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Ehrlich's test Wallace-Diamond test Schwartz-Watson test

chemical tests for urobilinogen

Ehrlich's test

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Wallace-Diamond test

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Schwartz-Watson test

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Schlesinger's test Schwartz-Watson test

chemical tests for urobilin

Schlesinger's test

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Schwartz-Watson test

⚗️ ➕

Hoesch's test

chemical test for porphobilinogen

Ham's test

• coproporphyrin

• uroporphyrin UV test

chemical tests for porphyrins

Ham's coproporphyrin test

⚗️ ➕

Ham's uroporphyrin test

⚗️ ➕

UV test

⚗️ ➕

Oliver's test Pettenkofer's test

qualitative chemical tests for bile acids

Hay's test

quantitative chemical test for bile acids

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Obermayer's test

chemical test for indican

⚗️ ➕

bromine water test ferric chloride test Blackberg & Wanger's test Thormalen's test

chemical tests for melanin

bromine water test

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