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Prokaryotic Genome Structure
Singular circular chromosome, contains little non-coding DNA.
Eukaryotic Genome Structure
Multiple long strands of chromosomes, contains significant amounts of non-coding DNA.
Gene Expression Control in Eukaryotes
Control can occur during transcription, post-transcriptional modification, translation, and post-translation.
Operon
A cluster of genes in prokaryotes that can be turned on or off to regulate transcription and protein production.
Common Features of Operons
Includes an operator, repressor protein, co-repressor/coreducer, promoter, regulator, and structural genes.
Function of the Operator in an Operon
The operator is the binding site for the repressor protein, which can inhibit transcription when bound.
Role of the Repressor Protein
When active, the repressor binds to the operator to prevent transcription.
Corepressor and Coreducer Function
Corepressors activate repressor proteins to inhibit transcription; coreducers deactivate them to allow transcription.
Tryptophan Control in Bacteria
The trp operon regulates tryptophan production, functioning as a repressible operon to prevent waste when enough is produced.
Tryptophan Feedback Inhibition
Occurs in the trp operon where excess tryptophan binds as a corepressor to halt transcription, preventing overproduction.
Glucose and Lactose Relationship
Lactose can be broken down by bacteria into glucose for energy.
Function of B-galactosidase
An enzyme that breaks lactose into allolactose and glucose.
Lac Operon and Breakdown of Lactose
Controls the breakdown of lactose and is considered inducible because it is activated in the presence of lactose.