Bacterial Culture, Sensitivity and Specimen Collection

A set of vocabulary flashcards covering essential terms and definitions related to bacterial culture, sensitivity testing, and specimen collection.

Define Bacterial Culture

A method used to determine the type(s) of bacteria present in a sample.

Can a bacterial culture be aerobic and anaerobic?

Yes

What are 4 reasons that we would culture bacteria?

• determine type(s)

• Determine if bacteria is pathogenic

• Determine appropriate Tx

• Determine if patient is carrier

What is the gold standard for determining antibiotic Tx and ensuring resolution of infection?

Culture & Sensitivity

What are 3 mediums for m=bacteria growth we can use for an in clinic aerobic bacterial culture

• agar plates

• Liquid broth

• Tubes w slant media

Non fastidious bacteria

• bacteria that requires minimal nutrients for growth

• Not fussy

Fastidious organisms

• require supplemental components for growth

What temp should the incubator be at?

35-37

How many days to plates get monitored for?

3

What is a Sensitivity Test

A test that determines which antibiotics bacteria are sensitive to and resistant to.

What is Minimum Inhibitory Concentration (MIC)

The lowest concentration of an antibiotic that would inhibit bacterial growth.

Bactericidal Antibiotic

An antibiotic that kills bacteria.

Bacteriostatic Antibiotic

An antibiotic that inhibits the growth or reproduction of bacteria.

Endogenous Bacteria

Microorganisms that are already living inside the body.

Exogenous Source

Bacteria that comes from outside the body.

Gram Stain

A method used to determine whether bacteria are gram-positive or gram-negative.

Cytocentesis

The gold standard for urine collection for a bacterial culture.

California Mastitis Test (C.M.T.)

A test performed when confirming mastitis that indicates positive results based on gel formation in milk after reagent addition.

Bronchoalveolar Lavage (BAL)

A procedure to obtain a sterile sample from the lower respiratory tract in suspected infections.

Catalase Test

A test used to distinguish between Staphylococcus spp. and Streptococcus spp. based on bubble formation in hydrogen peroxide.

Pasteurella Multocida

A gram-negative bacteria often associated with cat bites or abscesses.

Normal Flora

Microbial populations that normally inhabit certain areas of the body, such as the external ear.

Otitis Externa

An ear infection commonly found in dogs, often caused by gram-negative rods like Pseudomonas Aeruginosa.

Colony Features

Characteristics such as size, colour, shape, texture, elevation, and hemolysis used to describe an isolated colony grown on a culture medium.