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Key vocabulary terms and concise definitions covering the major concepts, structures, pigments, and processes involved in photosynthesis as presented in the notes.
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Photosynthesis
The process by which green plants, algae, and certain bacteria convert light energy, CO2 and water into chemical energy (glucose), releasing oxygen as a by-product.
Chloroplast
Plant cell organelle where photosynthesis occurs; contains chlorophyll, thylakoids, granum, and stroma.
Chlorophyll
Green pigment that absorbs blue and red light, driving the light-dependent reactions of photosynthesis.
Thylakoid
Disc-shaped membrane-bound sacs inside chloroplasts where the light-dependent reactions take place.
Granum (plural: grana)
A stack of thylakoids within the chloroplast; increases surface area for light absorption.
Stroma
Fluid-filled interior of a chloroplast where the Calvin cycle (light-independent reactions) occurs.
Light-dependent reactions
Stage of photosynthesis in the thylakoid membranes that uses light to produce ATP and NADPH and release O2.
Light-independent reactions (Calvin cycle)
Stage set in the stroma that uses ATP and NADPH to fix CO2 into carbohydrates.
Photolysis of water
Splitting of water by light energy during the light reactions to provide electrons, protons, and O2.
NADP+/NADPH
Nicotinamide adenine dinucleotide phosphate; NADP+ accepts electrons to form NADPH, a reducing agent for carbon fixation.
ATP
Adenosine triphosphate; energy currency produced during the light reactions to power the Calvin cycle.
RuBP (Ribulose-1,5-bisphosphate)
Five-carbon compound that acts as the CO2 acceptor in the Calvin cycle.
GP (Glycerate-3-phosphate)
Three-carbon intermediate formed when CO2 is fixed to RuBP during the Calvin cycle.
TP (Triose phosphate, PGAL/GAP)
Three-carbon sugar produced in the Calvin cycle; used to synthesize carbohydrates and regenerate RuBP.
Calvin cycle
Light-independent cycle in the stroma that fixes CO2 into carbohydrates using ATP and NADPH.
Carbohydrates (in photosynthesis context)
Sugars and starches produced from triose phosphates; include glucose, sucrose, and starch.
Glucose
A simple sugar produced from TP; a key carbohydrate for respiration and biosynthesis.
Sucrose
Disaccharide formed from glucose; transported through phloem to parts of the plant.
Starch
Storage polysaccharide formed from glucose, stored mainly in leaves when not immediately needed.
Cellulose
Structural polysaccharide synthesized from glucose; main component of plant cell walls.
Lipids
Fatty acids and glycerol derived from TP; used for membrane formation and energy storage.
Proteins
Macromolecules built from amino acids derived from GP/TP; used for enzymes, membranes, and growth.
Stomata
Pores in the leaf epidermis that regulate gas exchange (CO2 in, O2 out) and water loss.
Guard cells
Pair of cells surrounding a stomatal pore that control its opening and closing.
Cuticle
Waxy outer layer that reduces water loss from the leaf surface.
Epidermis
Outer protective cell layer of the leaf; can be transparent to allow light in.
Palisade mesophyll
Layer of tall, closely packed cells rich in chloroplasts; main site of photosynthesis.
Spongy mesophyll
Leaf layer with air spaces facilitating gas diffusion and photosynthesis.
Xylem
Vascular tissue that transports water and minerals from roots to leaves.
Phloem
Vascular tissue that transports sugars and nutrients from leaves to other parts of the plant.
Absorption spectrum
Graph showing which wavelengths of light are absorbed by pigments (e.g., chlorophyll).
Action spectrum
Graph showing the rate of photosynthesis at different wavelengths; often aligns with pigment absorption.
Destarching
Process of removing stored starch from a plant (usually by keeping in the dark) before experiments.
DCPIP test
Chemical test using DCPIP dye to indicate oxygen production during photosynthesis by a color change and/or reduction.
18O labeling
Use of the heavy oxygen isotope to trace the source of oxygen in the O2 produced during photosynthesis.
18O2 vs 16O2
Different isotopes used to determine whether O2 comes from water or CO2 in photosynthesis experiments.
RuBP regeneration
Part of the Calvin cycle where TP is used to regenerate RuBP to continue CO2 fixation.
Carbon fixation
Initial incorporation of CO2 into a five-carbon sugar (RuBP) to form a 6-carbon intermediate that splits into GP.
GP to TP reduction
GP is reduced to TP using ATP and NADPH in the Calvin cycle.
Photosynthetic pigments
Molecules like chlorophyll and carotenoids that absorb light and drive photosynthesis.
Carotenoids
Accessory pigments that broaden the spectrum of light absorbed and protect against light damage.