BIOL 203 Past Exam MCQ and Collaborative Quizzes

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1
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What type of bonds occur between functional groups to maintain the structure of a ꞵ-pleated sheet?

a. Van der Waals

b. Ionic

c. Disulfide

d. Covalent

e. Hydrogen.

e. Hydrogen.

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If a double-stranded DNA sample was composed of 30 percent adenine, what would be the percentage of cytosine?

A. 15

B. 20

C. 25

D. 50

E. None of the above

B. 20

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<p>Phosphorylase and amylase are enzymes that catalyze the hydrolysis of glycogen (pictured) and starch, respectively. What types of bonds do they break?</p><p>A. ⍺-1,4-glycosidic bonds</p><p>B. Hydrogen bonds</p><p>C. Ionic bonds</p><p>D. β-1,4-glycosidic bonds</p><p>E. Peptide bonds</p>

Phosphorylase and amylase are enzymes that catalyze the hydrolysis of glycogen (pictured) and starch, respectively. What types of bonds do they break?

A. ⍺-1,4-glycosidic bonds

B. Hydrogen bonds

C. Ionic bonds

D. β-1,4-glycosidic bonds

E. Peptide bonds

A. ⍺-1,4-glycosidic bonds

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Why is RNA less stable compared to DNA?
A. DNA contains a more reactive hydrogen group on the 2’ carbon of the sugar
B. RNA contains a more reactive hydroxyl group on the 2’ carbon of the sugar
C. RNA and DNA have no difference in stability
D. DNA has stronger phosphodiester bonds than RNA
E. RNA lacks complementary base pairing, so it degrades faster

B. RNA contains a more reactive hydroxyl group on the 2’ carbon of the sugar

5
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<p><span><span>The chemical reaction illustrated in the accompanying figure _______.<br>A. results in a β-1,4-glycosidic bond<br>B. is a hydrolysis reaction<br>C. results in a peptide bond<br>D. results in an α-1,4-glycosidic bond<br>E. joins two nucleotides together</span></span></p>

The chemical reaction illustrated in the accompanying figure _______.
A. results in a β-1,4-glycosidic bond
B. is a hydrolysis reaction
C. results in a peptide bond
D. results in an α-1,4-glycosidic bond
E. joins two nucleotides together

C. results in a peptide bond

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<p><span><span>If molecule C is the glucose transporter, GLUT-1, what does the above diagram indicate is happening?<br>A. Simple diffusion out of the cell.<br>B. Facilitated diffusion out of the cell.<br>C. Active transport out of the cell.<br>D. Active transport into the cell.<br>E. Facilitated diffusion into the cell</span></span></p>

If molecule C is the glucose transporter, GLUT-1, what does the above diagram indicate is happening?
A. Simple diffusion out of the cell.
B. Facilitated diffusion out of the cell.
C. Active transport out of the cell.
D. Active transport into the cell.
E. Facilitated diffusion into the cell

E. Facilitated diffusion into the cell

7
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Both proteins and DNA can be (i) denatured and (ii) undergo hydrolysis. Denaturation involves using heat to disrupt specific interactions whereas hydrolysis involves breaking bonds, which requires enzymes. What would happen to protein molecules treated with proteinase K?
A. The phosphodiester linkages of the sugar-phosphate backbone would be broken.
B. The two strands of the double helix would separate.
C. The interactions between the R-groups would be disrupted.
D. The peptide bonds would be broken.
E. The phosphate groups would be separated from the sugars.

D. The peptide bonds would be broken.

8
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Suppose a cell is placed in a solution with high concentration of potassium and no sodium. How would the cellular sodium-potassium pump function in this environment?
A. It would stop moving ions across the membrane.
B. It would continue using ATP to pump sodium out of the cell and potassium into the cell.
C. It would move sodium and potassium ions across the membrane, but no ATP would be used.
D. It would reverse the direction of pumping sodium and potassium ions to move them against
their gradients.
E. It would allow sodium and potassium to diffuse freely across the membrane down their
gradients

B. It would continue using ATP to pump sodium out of the cell and potassium into the cell.

9
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What determines the primary structure of an RNA molecule?
A. Complementary base pairing
B. Deoxyribonucleotide sequence
C. Stem-and-loop configuration
D. Hydrophobic interactions and hydrogen bonding
E. Ribonucleotide sequence

E. Ribonucleotide sequence

10
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Which of the following is not dependent upon hydrogen bonding interactions?
A. An α-helix in an integral membrane protein
B. Complementary base pairing in DNA
C. Peptidoglycan forming fibers in the bacterial cell wall
D. Cellulose forming fibers in the plant cell wall
E. Adhesion experienced by plants

C. Peptidoglycan forming fibers in the bacterial cell wall

11
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<p><span><span>The water molecule has many unique properties attributing to its importance in life. The polarity of the water molecule makes it an effective solvent for charged and polar solutes like the glucose molecule below. What is the attraction between the water molecules and glucose called, as indicated by the dashed lines?<br>A. A hydrogen bond<br>B. A polar covalent bond<br>C. An ionic bond<br>D. A van der Waals interaction<br>E. A hydrophobic interaction</span></span></p>

The water molecule has many unique properties attributing to its importance in life. The polarity of the water molecule makes it an effective solvent for charged and polar solutes like the glucose molecule below. What is the attraction between the water molecules and glucose called, as indicated by the dashed lines?
A. A hydrogen bond
B. A polar covalent bond
C. An ionic bond
D. A van der Waals interaction
E. A hydrophobic interaction

A. A hydrogen bond

12
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Given what you know about the chemical properties of the phospholipid bilayer, which of the following proteins is UNLIKELY to be found associated with the membrane?
A. A protein with a 20-30 amino acid stretch of alternating polar and nonpolar amino acids
B. A protein consisting of ONLY hydrophobic amino acids
C. A protein consisting of ONLY hydrophilic amino acids
D. A globular protein with nonpolar amino acid side chains folded into the interior of the protein
and hydrophilic side chains exposed on the surface.
E. None of the above

B. A protein consisting of ONLY hydrophobic amino acids

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<p><span><span>The molecule below shows the stem-loop structure for an RNA<br>molecule. Fill in the complementary bases to complete the stem.<br>A. 5’- UGGC-3’<br>B. 3’- CGGU-5’<br>C. 5’- UGCC-3’<br>D. 3’- GUCU-5’<br>E. None of the above</span></span></p>

The molecule below shows the stem-loop structure for an RNA
molecule. Fill in the complementary bases to complete the stem.
A. 5’- UGGC-3’
B. 3’- CGGU-5’
C. 5’- UGCC-3’
D. 3’- GUCU-5’
E. None of the above

A. 5’- UGGC-3’
B. 3’- CGGU-5’

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<p><span><span>A mutation occurs in this portion of a chromosome where cytosine replaces thymine. What impact do you think this will have on the DNA structure below?<br>A. This will have very little effect on the DNA secondary (2º) structure.<br>B. This will cause the phosphodiester bonds to break, and the DNA will hydrolyze.<br>C. This will cause the DNA secondary (2º) structure to denature or unzip.<br>D. This will cause a bulge in the DNA secondary (2º) structure.<br>E. This will cause a major distortion in the DNA secondary (2º) structure</span></span></p>

A mutation occurs in this portion of a chromosome where cytosine replaces thymine. What impact do you think this will have on the DNA structure below?
A. This will have very little effect on the DNA secondary (2º) structure.
B. This will cause the phosphodiester bonds to break, and the DNA will hydrolyze.
C. This will cause the DNA secondary (2º) structure to denature or unzip.
D. This will cause a bulge in the DNA secondary (2º) structure.
E. This will cause a major distortion in the DNA secondary (2º) structure

A. This will have very little effect on the DNA secondary (2º) structure.

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<p><span><span>According to the diagram to the right, Na+ is experiencing ______________ and Glucose is experiencing _____________?<br>A. Facilitated diffusion; passive<br>transport<br>B. Active transport; secondary active<br>transport<br>C. Secondary active transport;<br>facilitated diffusion<br>D. Passive transport; secondary active<br>transport<br>E. None of the above</span></span></p>

According to the diagram to the right, Na+ is experiencing ______________ and Glucose is experiencing _____________?
A. Facilitated diffusion; passive
transport
B. Active transport; secondary active
transport
C. Secondary active transport;
facilitated diffusion
D. Passive transport; secondary active
transport
E. None of the above

D. Passive transport; secondary active transport

16
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How do the α and forms of glucose differ?
A. The oxygen atom inside the ring is located in a different position.
B. Their ring structures differ in the location of a hydroxyl group.
C. Their linear structures differ in the location of a hydroxyl group.
D. The α form can be involved in 1,4- and 1,6-glycosidic linkages; the
form can participate only in 1,4 linkages

B. Their ring structures differ in the location of a hydroxyl group.

17
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Mad cow disease is an infection disease where one misfolded protein causes all other copies of the protein to begin misfolding. This is an example of a disease impacting ____ structure.
A. Primary
B. Secondary
C. Tertiary
D. Quaternary
E. A and B

C. Tertiary

18
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<p><span><span>The effect of diet on human health is often evaluated using animal models. Researchers tested the impact of drinking fructose solutions on memory by using rats trained to find an escape chamber in a maze. The effect of eating omega-3 fatty acids was also tested. The rats were fed various diets consisting of chow and drinking<br>water. After six weeks on the diets, the rats were tested again on the same maze, and their escape times were recorded and graphed below. The researchers hypothesize that excessive fructose impairs the ability to recall since fructose metabolism is less<br>controlled, which can lead to neuroinflammation in the brain. What can you conclude from these results about learning retention in rats?<br>A. These results suggest that fructose impaired the rats’ ability to recall what they had<br>learned about finding the escape chamber with statistical significance.<br>B. These results suggest that omega-3 fatty acids impaired the rats’ ability to recall what they had<br>learned about finding the escape chamber with statistical significance.<br>C. These results suggest that fructose and omega-3 fatty acids impaired the rats’ ability to recall<br>what they had learned about finding the escape chamber with statistical significance.<br>D. These results suggest that omega-3 fatty acids enhanced the rats’ ability to recall what they had<br>learned about finding the escape chamber but without statistical significance.<br>E. These results suggest that fructose and omega-3 fatty acids did not have a statistically<br>significant effect on the rats’ ability to recall what they had learned about finding the escape<br>chamber</span></span></p>

The effect of diet on human health is often evaluated using animal models. Researchers tested the impact of drinking fructose solutions on memory by using rats trained to find an escape chamber in a maze. The effect of eating omega-3 fatty acids was also tested. The rats were fed various diets consisting of chow and drinking
water. After six weeks on the diets, the rats were tested again on the same maze, and their escape times were recorded and graphed below. The researchers hypothesize that excessive fructose impairs the ability to recall since fructose metabolism is less
controlled, which can lead to neuroinflammation in the brain. What can you conclude from these results about learning retention in rats?
A. These results suggest that fructose impaired the rats’ ability to recall what they had
learned about finding the escape chamber with statistical significance.
B. These results suggest that omega-3 fatty acids impaired the rats’ ability to recall what they had
learned about finding the escape chamber with statistical significance.
C. These results suggest that fructose and omega-3 fatty acids impaired the rats’ ability to recall
what they had learned about finding the escape chamber with statistical significance.
D. These results suggest that omega-3 fatty acids enhanced the rats’ ability to recall what they had
learned about finding the escape chamber but without statistical significance.
E. These results suggest that fructose and omega-3 fatty acids did not have a statistically
significant effect on the rats’ ability to recall what they had learned about finding the escape
chamber

A. These results suggest that fructose impaired the rats’ ability to recall what they had
learned about finding the escape chamber with statistical significance.

19
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A number of systems for pumping ions across membranes are powered by ATP. Such ATP-powered pumps are often called ATPases, although they do not often hydrolyze ATP unless they are simultaneously transporting ions. Because small increases in calcium ions in the cytosol can trigger several different intracellular reactions, cells keep the cytosolic calcium concentration quite low under normal conditions, using ATP-powered calcium pumps. For example, muscle cells transport calcium from the cytosol into the membranous system called the sarcoplasmic reticulum (SR). If a resting muscle cell's cytosol has a free calcium ion concentration of 10-7 while the
concentration in the SR is 10
-2, then how is the ATPase acting?
A. ATPase activity must be powering an inflow of calcium from the outside of the cell into the SR.
B. ATPase activity must be pumping calcium from the cytosol to the SR against the
concentration gradient.
C. ATPase activity must be opening a channel for the calcium ions to diffuse back into the SR along the concentration gradient.
D. ATPase activity must be routing calcium ions from the SR to the cell's environment

B. ATPase activity must be pumping calcium from the cytosol to the SR against the
concentration gradient.

20
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Proteins and nucleic acids have definite directionality. Stated another way, one end of the molecule is different from the other end. How are the ends of a protein described?
A. One end has an unlinked 3' hydroxyl group; the other end has an unlinked 5' phosphate group.
B. One end has an unlinked carboxyl group; the other end has an unlinked amino group.
C. One end has an unlinked amino group; the other end has an unlinked hydroxyl group.
D. One end has an unlinked phosphate group; the other end has an unlinked carboxyl group.
E. One end has an unlinked 3' phosphate group; the other end has an unlinked 5' hydroxyl group

B. One end has an unlinked carboxyl group; the other end has an unlinked amino group.

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<p><span><span>Compare the two cells shown in this image. Which of the following statements is correct?<br>A. The right cell has twice as many chromosomes as the left cell, but both cells contain the same amount of DNA.<br>B. The right cell contains twice the number of chromosomes and amount of DNA as the left cell.<br>C. Both cells contain the same number of chromosomes and the same amount of DNA.<br>D. The number of chromosomes in both cells is the same, but the right cell contains twice the amount<br>of DNA as the left cell</span></span></p>

Compare the two cells shown in this image. Which of the following statements is correct?
A. The right cell has twice as many chromosomes as the left cell, but both cells contain the same amount of DNA.
B. The right cell contains twice the number of chromosomes and amount of DNA as the left cell.
C. Both cells contain the same number of chromosomes and the same amount of DNA.
D. The number of chromosomes in both cells is the same, but the right cell contains twice the amount
of DNA as the left cell

A. The right cell has twice as many chromosomes as the left cell, but both cells contain the same amount of DNA.

22
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Which of the following statements correctly identify a role of the ATP produced in the light-capturing reactions?
I. ATP from the light-capturing reactions is used by rubisco to fix CO2 to ATP
II. ATP from the light capturing reactions is used to regenerate RuBP from G3P molecules
III. ATP from the light capturing reactions serves the same role as ATP produced by mitochondria
IV. ATP from the light capturing reactions is used to produce G3P molecules during reduction
A. I only
B. II and III only
C. IV only
D. II and IV only
E. All of the above

D. II and IV only

23
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Imagine you expose chloroplast to red photons and observe low O2 production, but high ATP production. Which of the following best explains this observation?
A. The electrons from water are directly transferred to NADP+, which is used to generate ATP
B. PS II is not splitting water, and the ATP is being produced by cycling electrons via PSI
C. O2 is being converted to water as a terminal electron acceptor in the production of ATP
D. Electron transport has stopped, and ATP is being produced by the Calvin Cycle
E. None of the above

B. PS II is not splitting water, and the ATP is being produced by cycling electrons via PSI

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<p><span><span>What if pyruvate oxidation is suddenly inhibited, what will happen to the levels of oxaloacetate and citric acid (citrate) in the citric acid cycle shown in the accompanying figure to the right?<br>A. Oxaloacetate will decrease, and citric acid will accumulate.<br>B. Oxaloacetate will accumulate, and citric acid will decrease.<br>C. Both oxaloacetate and citric acid will decrease.<br>D. Both oxaloacetate and citric acid will accumulate</span></span></p>

What if pyruvate oxidation is suddenly inhibited, what will happen to the levels of oxaloacetate and citric acid (citrate) in the citric acid cycle shown in the accompanying figure to the right?
A. Oxaloacetate will decrease, and citric acid will accumulate.
B. Oxaloacetate will accumulate, and citric acid will decrease.
C. Both oxaloacetate and citric acid will decrease.
D. Both oxaloacetate and citric acid will accumulate

B. Oxaloacetate will accumulate, and citric acid will decrease.

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Reactions that require ATP take place in ________.
A. the light reactions only
B. the Calvin cycle only
C. Glycolysis only
D. Both A and C
E. Both B and C

E. Both B and C

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Cells get past cell-cycle checkpoints by ________________.
A. Cdks working on their own to phosphorylate other proteins
B. Cyclins working on their own to phosphorylate other proteins
C. Cdks bound to cyclins phosphorylating other proteins
D. Cyclins being present at the same level during the entire cell cycle

C. Cdks bound to cyclins phosphorylating other proteins

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<p><span><span>Please examine the metabolic pathway below: Imagine that you observe a buildup of Intermediate A and a gradual decrease in the amount of<br>Intermediate B in a cell over time. Which of the following scenarios most likely accounts for this?<br>A. allosteric inhibition of Enzyme 1 by Product<br>B. competitive inhibition of Enzyme 2 by Intermediate B<br>C. allosteric inhibition of Enzyme 2 by Product<br>D. competitive inhibition of Enzyme 1 by Intermediate B</span></span></p>

Please examine the metabolic pathway below: Imagine that you observe a buildup of Intermediate A and a gradual decrease in the amount of
Intermediate B in a cell over time. Which of the following scenarios most likely accounts for this?
A. allosteric inhibition of Enzyme 1 by Product
B. competitive inhibition of Enzyme 2 by Intermediate B
C. allosteric inhibition of Enzyme 2 by Product
D. competitive inhibition of Enzyme 1 by Intermediate B

C. allosteric inhibition of Enzyme 2 by Product

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What would happen if all of the cells in a developing frog embryo expressed the same type of cadherin on their surfaces?
A. Cells would easily dissociate from each other as the wrong cadherins would bind to each other.
B. All the channels between cells would be blocked, so there would be no cell-to-cell communication.
C. Antibodies would not be able to bind to cadherin as all the receptors would be blocked.
D. All of the cells in the embryo would be capable of attaching to one another regardless of the cell type

D. All of the cells in the embryo would be capable of attaching to one another regardless of the cell type

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<p><span><span>Which of the following amino acids has the highest potential energy?<br>A. isoleucine<br>B. serine<br>C. aspartic acid<br>D. cysteine</span></span></p>

Which of the following amino acids has the highest potential energy?
A. isoleucine
B. serine
C. aspartic acid
D. cysteine

A. isoleucine

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<p><span><span>The graph below shows the rate of product formation in an enzyme-catalyzed reaction as a function of varying substrate concentration, with the concentration of<br>enzyme constant. What if this enzyme was regulated by competitive inhibition and the regulatory molecule is left out of the reactions. Which graph would best represent<br>the rate of product formation.<br>A. Graph A<br>B. Graph B<br>C. Neither graph A nor B</span></span></p>

The graph below shows the rate of product formation in an enzyme-catalyzed reaction as a function of varying substrate concentration, with the concentration of
enzyme constant. What if this enzyme was regulated by competitive inhibition and the regulatory molecule is left out of the reactions. Which graph would best represent
the rate of product formation.
A. Graph A
B. Graph B
C. Neither graph A nor B

B. Graph B

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In cell signaling, signals (ligands) are classified as lipid-soluble and lipid insoluble. Adrenaline is classified as a lipid insoluble signal, and its receptors are activated when ________.
A. the signal crosses the membranes of target cells containing the intracellular receptors
B. the signal binds to G-protein-coupled receptors, present only on target cells
C. target cells produce second messengers in response to the signal
D. target cells possess the cytosolic enzymes that transduce the signal

B. the signal binds to G-protein-coupled receptors, present only on target cells

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<p><span><span>Using your knowledge of the cell cycle and the following diagram, indicate which of the following will lead to formation of a tumor (uncontrolled cell division).<br>1. Ras with a GTP that cannot be hydrolyzed<br>2. Overexpression of MPF, the M-phase cyclin-Cdk<br>3. Nonfunctional E2F protein<br>4. Under-expression of G1 cyclin<br>5. Nonfunctional Rb protein<br>A. 2, 3, 4 only<br>B. 2 and 5 only<br>C. 1, 2, 5 only<br>D. 1 and 5 only<br>E. 1, 2, 3, 4, 5</span></span></p>

Using your knowledge of the cell cycle and the following diagram, indicate which of the following will lead to formation of a tumor (uncontrolled cell division).
1. Ras with a GTP that cannot be hydrolyzed
2. Overexpression of MPF, the M-phase cyclin-Cdk
3. Nonfunctional E2F protein
4. Under-expression of G1 cyclin
5. Nonfunctional Rb protein
A. 2, 3, 4 only
B. 2 and 5 only
C. 1, 2, 5 only
D. 1 and 5 only
E. 1, 2, 3, 4, 5

D. 1 and 5 only

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The normal heart rhythm depends on adjacent cardiac muscle cells sharing ions, second messengers, and electrical impulses. __________________ are the membrane channels that connect cardiac muscle cells, providing this chemical and electrical communication leading to the synchronous contraction of the heart.
A. Plasmodesmata
B. Gap junctions
C. Desmosomes
D. Tight junctions
E. Extra cellular matrix

B. Gap junctions

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Not all signaling between cells require transduction. Which one of the following signals would be processed without transduction?
A. A lipid-soluble signal
B. A Notch signal
C. A signal that binds to a receptor in the cell membrane
D. A lipid-insoluble signal

A. A lipid-soluble signal

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Consider this pathway: adrenaline → G-protein-coupled receptor → G-protein-GTP → adenylyl cyclase → cAMP → Protein Kinase A. The second messenger in this pathway is ________.
A. cAMP
B. G-protein-GTP
C. Protein Kinase A
D. adenylyl cyclase
E. G-protein-coupled receptor

A. cAMP

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Scientists experimentally modified the gene encoding the HER2 receptor by removing all zip codes/tags/signals and added a nuclear export signal and a mannose-6-phosphate tag. What is the normal location of the HER2 receptor and its possible new location, respectively?
A. Cytosol; Nucleus
B. Plasma Membrane; Lysosome
C. Cytosol; Lysosome
D. Plasma Membrane; Cytosol
E. None of the above

D. Plasma Membrane; Cytosol

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Steroid hormones bind to soluble receptors in the cytosol of the cell and alter their conformation. The hormone-receptor complex is then transported into the nucleus, where it can directly bind DNA and affect gene expression. To get from the location where the receptor binds the hormone to its site where it binds DNA, the hormone-receptor complex must ________.
A. contain a mannose-6-phosphate tag
B. contain an ER signal sequence
C. diffuse to its new location
D. contain an NLS (nuclear localization signal)
E. become phosphorylated

D. contain an NLS (nuclear localization signal)

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Axonemal dynein is responsible for the movement of cilia and flagella, whereas cytoplasmic dynein facilitates movement of organelles and other cargo, just like kinesin. These motor proteins walk on microtubules carrying their cargo. If a cell receives a vesicle by endocytosis, which motor protein would deliver the vesicle to its destination in the cell and how?
A. Kinesin walking towards the plus end of the microtubule.
B. Kinesin walking towards the minus end of the microtubule.
C. Dynein walking towards the plus end of the microtubule.
D. Dynein walking towards the minus end of the microtubule

D. Dynein walking towards the minus end of the microtubule

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<p><span><span>You eat a large plate of non-fat pasta for a late evening snack. Using your knowledge of the central role cellular respiration plays in metabolism and the figure below as a reference, indicate if it is possible for the carbon atoms contained in the pasta to end up in the indicated molecules.<br>1. Cellulose stored in your muscle cells<br>2. A sodium ion actively transported by the sodium/potassium-ATPase<br>3. A lysosomal hydrolase<br>4. Water made in cellular respiration<br>5. Oxygen used in cellular respiration<br>A. 3 only<br>B. 2 and 3 only<br>C. 1, 3, 5 only<br>D. 2, 3, 4 only<br>E. 1, 2, 3, 4, 5</span></span></p>

You eat a large plate of non-fat pasta for a late evening snack. Using your knowledge of the central role cellular respiration plays in metabolism and the figure below as a reference, indicate if it is possible for the carbon atoms contained in the pasta to end up in the indicated molecules.
1. Cellulose stored in your muscle cells
2. A sodium ion actively transported by the sodium/potassium-ATPase
3. A lysosomal hydrolase
4. Water made in cellular respiration
5. Oxygen used in cellular respiration
A. 3 only
B. 2 and 3 only
C. 1, 3, 5 only
D. 2, 3, 4 only
E. 1, 2, 3, 4, 5

A. 3 only

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Canine phosphofructokinase (PFK) deficiency afflicts Springer spaniels, affecting an estimated 10 percent of the breed. Given its critical role in glycolysis, one implication of the genetic defect resulting
in PFK deficiency in dogs is ________.
A. Early embryonic mortality (death)
B. Elevated glucose levels in the dog’s blood
C. An intolerance for exercise
D. A reduced life span

C. An intolerance for exercise

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What if the gene encoding primase is defective due to a frameshift mutation in a cell preparing to undergo replication of its DNA. What will the leading and lagging strand of DNA at a single replication fork look like?
A. leading strand will be complete, lagging strand will consist of Okazaki fragments without RNA primers
B. leading strand will be complete, lagging strand will consist of Okazaki fragments with RNA primers
C. leading and lagging strands will both be complete
D. leading and lagging strands will both be non-existent

D. leading and lagging strands will both be non-existent

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What would happen if you used a human DNA polymerase III in PCR rather than Taq polymerase?
A. Since all DNA polymerases are able to build new strands of DNA, PCR would continue as normal.
B. The human DNA polymerase would denature at the high temperatures required to break the hydrogen bonds holding complementary DNA strands together – PCR would fail
C. The human DNA polymerase would be unable to synthesize new strands of DNA without the addition of primase – PCR would fail
D. The human DNA polymerase would be unable to synthesize the lagging strand without the addition of helicase – PCR would fai

B. The human DNA polymerase would denature at the high temperatures required to break the hydrogen bonds holding complementary DNA strands together – PCR would fail

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<p><span><span>Identify the lagging strand during DNA replication in the figure below.<br>A. a<br>B. b<br>C. c<br>D. d</span></span></p>

Identify the lagging strand during DNA replication in the figure below.
A. a
B. b
C. c
D. d

C. c

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<p><span><span>Use the following diagram to answer questions 4, 5 and 6.<br>Which of the following are required for the specific process occurring above?<br>1. DNA<br>2. rRNA<br>3. mRNA<br>4. tRNA<br>5. Charged tRNA<br>6. snRNPs (snurps)<br>7. DNA Polymerase<br>8. RNA Polymerase<br>A. 1 &amp; 7<br>B. 2, 3, &amp; 5<br>C. 1, 3, 6, &amp; 8<br>D. 1, 2, 3, 5, &amp; 8<br>E. 2, 3, 4, &amp; 8</span></span></p>

Use the following diagram to answer questions 4, 5 and 6.
Which of the following are required for the specific process occurring above?
1. DNA
2. rRNA
3. mRNA
4. tRNA
5. Charged tRNA
6. snRNPs (snurps)
7. DNA Polymerase
8. RNA Polymerase
A. 1 & 7
B. 2, 3, & 5
C. 1, 3, 6, & 8
D. 1, 2, 3, 5, & 8
E. 2, 3, 4, & 8

B. 2, 3, & 5

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<p><span><span>Using the 5th codon in the diagram above, find the sequences for the DNA template strand corresponding to the codon and the anticodon.<br>A. DNA template: 5’-ACC-3’; Anticodon: 5’-ACC-3’<br>B. DNA template: 5’-GCT-3’; Anticodon: 5’-GCU-3’<br>C. DNA template: 5’-CCA-3’; Anticodon: 5’-CCA-3’<br>D. DNA template: 5’-TCG-3’; Anticodon: 5’-UCG-3’</span></span></p>

Using the 5th codon in the diagram above, find the sequences for the DNA template strand corresponding to the codon and the anticodon.
A. DNA template: 5’-ACC-3’; Anticodon: 5’-ACC-3’
B. DNA template: 5’-GCT-3’; Anticodon: 5’-GCU-3’
C. DNA template: 5’-CCA-3’; Anticodon: 5’-CCA-3’
D. DNA template: 5’-TCG-3’; Anticodon: 5’-UCG-3’

B. DNA template: 5’-GCT-3’; Anticodon: 5’-GCU-3’

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In a ribosome, a mutation has changed a nucleotide in the active site of the large subunit rRNA. The ribozyme cannot cause the formation of a covalent bond. Which of the following might be a reasonable explanation of how this might affect translation?
A. Transcription would continue, producing a pre-mRNA because ribozyme function is not needed for forming phosphodiester bonds.
B. Translation would continue, producing a series of free amino acids, all unattached because the ribozyme is unable to form peptide bonds.
C. Transcription would continue, but no mature mRNA would be produced because ribozymes are needed for splicing out introns in the RNA.
D. Translation would stop because ribozymes are needed to attach the codons with the anticodons.

B. Translation would continue, producing a series of free amino acids, all unattached because the ribozyme is unable to form peptide bonds.

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<p><span><span>Please examine the pedigree below, which illustrates the pattern of inheritance for a particular condition. Individuals who exhibit the condition of interest are shaded, while individuals who do not exhibit the condition of interest are unshaded. Given this information, what is the most likely genotype of the individual indicated with a question mark?<br>A. aa<br>B. Aa<br>C. X</span><sup><span>A</span></sup><span>X</span><sup><span>a</span></sup><span><br>D. X</span><sup><span>A</span></sup><span>X</span><sup><span>A</span></sup></span></p>

Please examine the pedigree below, which illustrates the pattern of inheritance for a particular condition. Individuals who exhibit the condition of interest are shaded, while individuals who do not exhibit the condition of interest are unshaded. Given this information, what is the most likely genotype of the individual indicated with a question mark?
A. aa
B. Aa
C. X
AXa
D. X
AXA

C. XAXa

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Suppose that you want to introduce the human insulin gene into bacteria to synthesize the insulin protein. Which of the following are pre-requisites for gene expression to occur?
A. The exons need to be removed from the mRNA.
B. A eukaryotic promoter must be present in the mRNA.
C. Eukaryotic ribosomes and tRNAs must be provided.
D. The introns need to be removed and a bacterial promoter must be present in the mRNA.
E. The introns need to be removed, a bacterial promoter must be present in the mRNA, and eukaryotic ribosomes and tRNAs must be provided

D. The introns need to be removed and a bacterial promoter must be present in the mRNA.

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Which of the following types of mutation, resulting in an error in the mRNA just after the AUG start of translation, is likely to have the most harmful effect on the polypeptide product?
A. A deletion of a codon
B. A deletion of two nucleotides
C. A substitution of the third nucleotide in an ACC codon
D. A substitution of the first nucleotide of a GGG codon
E. An insertion of a codon

B. A deletion of two nucleotides

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Which of the following is an exception to the central dogma?
A. The discovery of RNA viruses that synthesize DNA from RNA using reverse transcriptase
B. The discovery that DNA contains the instructions for making proteins
C. The discovery of ribozymes
D. The discovery of the Wobble Hypothesis

A. The discovery of RNA viruses that synthesize DNA from RNA using reverse transcriptase

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<p><span><span>Please examine the following four cells, each of which represents four chromosomes in a cell undergoing mitosis. Imagine that shared color reflects chromosomes that are derived from the same parent and that genes are represented as letters (A/a or B/b) next to the locus at which each is found.</span></span></p><p><span><span>Which cell illustrates a possible arrangement of genes on chromosomes for this individual?<br>A. (a)<br>B. (b)<br>C. (c)<br>D. (d)</span></span></p>

Please examine the following four cells, each of which represents four chromosomes in a cell undergoing mitosis. Imagine that shared color reflects chromosomes that are derived from the same parent and that genes are represented as letters (A/a or B/b) next to the locus at which each is found.

Which cell illustrates a possible arrangement of genes on chromosomes for this individual?
A. (a)
B. (b)
C. (c)
D. (d)

A. (a)

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In a Eukaryotic Gene “X”, a mutation changes the first AUG (for Methionine) in the mRNA to AUA.
Of the following, which would be more likely to happen in this cell?
A. The mRNA would remain inside the nucleus and no translation would occur.
B. Translation would begin normally, and at the same location.
C. Translation would NOT occur, because the first start codon AUG has been altered.
D. If there is a second AUG, translation would begin here, and the protein would be shorter.

D. If there is a second AUG, translation would begin here, and the protein would be shorter.

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<p><span><span>In an experimental situation, a student researcher inserts an mRNA molecule into the cytoplasm of a eukaryotic cell after she has removed its 5’ cap and poly-A tail. Which of the following would you expect her to find?<br>A. The mRNA is quickly converted into a ribosomal subunit.<br>B. The cell adds a new poly-A tail to the mRNA.<br>C. The mRNA attaches to a ribosome and is translated, but more slowly.<br>D. The molecule is digested by enzymes because it is not protected at the 5’ end.</span></span></p>

In an experimental situation, a student researcher inserts an mRNA molecule into the cytoplasm of a eukaryotic cell after she has removed its 5’ cap and poly-A tail. Which of the following would you expect her to find?
A. The mRNA is quickly converted into a ribosomal subunit.
B. The cell adds a new poly-A tail to the mRNA.
C. The mRNA attaches to a ribosome and is translated, but more slowly.
D. The molecule is digested by enzymes because it is not protected at the 5’ end.

D. The molecule is digested by enzymes because it is not protected at the 5’ end.

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Which of the following molecules makes a “charged” tRNA molecule?
A. DNA polymerase
B. RNA polymerase
C. Primase
D. Ribozyme
E. Aminoacyl t-RNA synthetase

E. Aminoacyl t-RNA synthetase

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Suppose that you have a mutation that modifies the nucleotides at the exon-intron borders. This results in a mature mRNA that includes an intron. What would be the result of this at the organismal level?
A. The organism would have no effect, since the ribosomes cannot bind to messages containing introns.
B. The organism would have no effect, since there are no anticodons in the tRNAs that correspond to the codons in the intron mRNA.
C. The introns sequences would get removed during posttranslational modifications.
D. The information in the intron would be translated and this would result in additional amino acids being incorporated into the protein and possibly frameshift mutations.

D. The information in the intron would be translated and this would result in additional amino acids being incorporated into the protein and possibly frameshift mutations.

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In humans, Xeroderma Pigmentosum (XP) is a disorder where individuals develop thymine dimer lesions caused by exposure to ultraviolet light. Given the damage caused by UV radiation, the kind of gene affected in those with XP is one whose gene product is involved with ________.
A. mending of double-strand breaks in the DNA backbone
B. breakage of cross-strand covalent bonds
C. the ability to excise single-strand damage and replace it
D. the removal of double-strand damaged areas
E. causing affected skin cells to undergo apoptosis

C. the ability to excise single-strand damage and replace it

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Telomere shortening puts a limit on the number of times a cell can divide. Research has shown that telomerase can extend the life span of cultured human cells. How might adding telomerase affect cellular aging?
A. Telomerase will speed up the rate of cell proliferation.
B. Telomerase eliminates telomere shortening and slows down aging.
C. Telomerase shortens telomeres, which delays cellular aging.
D. Telomerase would have no effect on cellular aging

B. Telomerase eliminates telomere shortening and slows down aging.

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<p><span><span><br>Imagine that a wealthy man dies and leaves a large inheritance to his wife and daughter. Following his death, two men come forward, both claiming to be the<br>long-lost son placed up for adoption by the rich man and his wife before they were married. To evaluate the truthfulness of each claim, a judge orders that DNA markers for each man be compared against the mother and daughter of the rich man. Based on the results of this analysis (shown in the gel on the right), what can you conclude about the likelihood that each man is this long-lost son?<br>A. “Son” 1 could be the long-lost son, while “Son” 2 can be definitively excluded<br>B. “Son” 2 could be the long-lost son, while “Son” 1 can be definitively excluded<br>C. Neither man is the long-lost son<br>D. Both men could be the long-lost son; neither can be ruled out and further analysis is needed</span></span></p>


Imagine that a wealthy man dies and leaves a large inheritance to his wife and daughter. Following his death, two men come forward, both claiming to be the
long-lost son placed up for adoption by the rich man and his wife before they were married. To evaluate the truthfulness of each claim, a judge orders that DNA markers for each man be compared against the mother and daughter of the rich man. Based on the results of this analysis (shown in the gel on the right), what can you conclude about the likelihood that each man is this long-lost son?
A. “Son” 1 could be the long-lost son, while “Son” 2 can be definitively excluded
B. “Son” 2 could be the long-lost son, while “Son” 1 can be definitively excluded
C. Neither man is the long-lost son
D. Both men could be the long-lost son; neither can be ruled out and further analysis is needed

A. “Son” 1 could be the long-lost son, while “Son” 2 can be definitively excluded

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<p><span><span>Please examine cells 1-3 below and statements (I - V) that follow. If all three cells can be found in the same organism, which of the following statements about these three cells is correct?<br>I. Cell 1 is undergoing meiosis, while Cells 2 and 3 are undergoing mitosis.<br>II. Cells 1 and 3 will give rise to gametes, while Cell 2 will give rise to a somatic cell.<br>III. Cells 1 and 2 are diploid, while Cell 3 is haploid.<br>IV. Chromosomes are replicated in Cell 1 and unreplicated in Cells 2 and 3.<br>V. Cells 1, 2, and 3 are all eukaryotic.<br>A. Statements (I), (II), and (III) are correct.<br>B. Statements (I) and (IV) are correct.<br>C. Statements (II) and (III) are correct.<br>D. Statements (II), (III), and (V) are correct.</span></span></p>

Please examine cells 1-3 below and statements (I - V) that follow. If all three cells can be found in the same organism, which of the following statements about these three cells is correct?
I. Cell 1 is undergoing meiosis, while Cells 2 and 3 are undergoing mitosis.
II. Cells 1 and 3 will give rise to gametes, while Cell 2 will give rise to a somatic cell.
III. Cells 1 and 2 are diploid, while Cell 3 is haploid.
IV. Chromosomes are replicated in Cell 1 and unreplicated in Cells 2 and 3.
V. Cells 1, 2, and 3 are all eukaryotic.
A. Statements (I), (II), and (III) are correct.
B. Statements (I) and (IV) are correct.
C. Statements (II) and (III) are correct.
D. Statements (II), (III), and (V) are correct.

D. Statements (II), (III), and (V) are correct.

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In Labrador retrievers, primary coat color (black, chocolate, or yellow) is determined by the specific combination of alleles that an individual possesses at two genes. Variation at one gene, TYRP1, determines the amount of eumelanin that an individual produces, with one allele (B) coding for high enzymatic activity resulting in high eumelanin production (leading to black pigmentation) and the other (b) coding for low enzymatic activity resulting in low eumelanin production (leading to chocolate pigmentation). Variation at a second gene, MC1R, determines whether this pigment is deposited into
the fur. At this locus, allele E codes for a functional receptor that allows eumelanin deposition to occur, while allele e codes for a non-functional receptor that prevents eumelanin deposition from occurring. Imagine that two dogs, one with genotype Bbee, and the other with genotype BBEe, are crossed. What coat color ratio is expected in the puppies?
A. 100% black
B. 50% black, 50% chocolate
C. 50% black, 50% yellow
D. 50% black, 25% chocolate, 25% yellow

C. 50% black, 50% yellow

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Read the following sentences, and then choose one option below:

  1. “In this study, we tested the hypothesis that the cause of muscle mass loss after nerve damage is an increase in the protein TRB3.”

  2. “In this study, we tested the hypothesis that treatment with an antibody that blocks the enzymatic activity of protein PAPP-A will reduce atherosclerotic plaque progression.”

  3. "In this study, we tested the hypothesis that Creutzfeldt-Jakob disease is caused by misfolded prion proteins (PrPSc) and leads to rapid neurodegeneration.”

Option A: Both 1 and 2 are hypotheses.

Option B: 1 is a hypothesis, but 2 is a prediction.

Option C 1 is a prediction, but 2 is a hypothesis.

Option D Both 1 and 3 are hypotheses

Option E Both 2 and 3 are hypotheses 

Option B: 1 is a hypothesis, but 2 is a prediction.

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Both starch and cellulose are found in plants. However, enzymes that readily break starch apart cannot hydrolyze the bonds found in cellulose. Why is this logical?

Option A: Starch is held together by hydrogen bonding, not covalent bonding.

Option B: Cellulose molecules are highly branched, and enzymes are too bulky to fit.

Option C: The geometry of the bonds is different, and the shapes of enzyme active sites are highly specific.

Option D: Starch is held together by peptide bonds, not glycosidic linkages.

Option C: The geometry of the bonds is different, and the shapes of enzyme active sites are highly specific.

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<p><span><span>Use the bacterial ruler to estimate the length of the double-sided arrow in this image. You may take a screen grab if you would like to annotate the image on another app to estimate the length of the arrow.</span></span></p><p><span><span>Option A: </span></span>240 µm</p><p><span><span>Option B: </span></span>120 µm</p><p><span><span>Option C: </span></span>0.24 µm</p><p><span><span>Option D: </span></span>0.12 µm</p>

Use the bacterial ruler to estimate the length of the double-sided arrow in this image. You may take a screen grab if you would like to annotate the image on another app to estimate the length of the arrow.

Option A: 240 µm

Option B: 120 µm

Option C: 0.24 µm

Option D: 0.12 µm

Option C: 0.24 µm

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If one strand of a DNA molecule has the sequence of bases 5'-TATTCGA-3', the complementary strand sequence would read _____.

Option A: 5'-ATAAGCT-3'

Option B: 3'-UAUUGCU-5'

Option C: 5'-UAUUGCU-3'

Option D: 5'-TCGAATA-3’

Option D: 5'-TCGAATA-3’

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Suppose a cell contains the enzymes sucrase and fructase, and sucrose is introduced into the cell. Predict what would happen to the concentration of monosaccharides in the cell over time.

Option A: Fructose and sucrose would both decrease.

Option B: Fructose would decrease and glucose would accumulate.

Option C: Fructose and glucose would both decrease over time.

Option D: Sucrose and glucose would both decrease over time.

Option B: Fructose would decrease and glucose would accumulate.

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Water molecules can adhere to biological surfaces such as cell membranes and extracellular matrices. Which of the following best explains the significance of this adhesion?

Option A: Adhesion allows water to form hydrogen bonds with the hydrocarbon backbone of cellulose

Option B: Adhesion via hydrogen bonding to polar groups maintains hydration and proper membrane function

Option C: Adhesion allows water to form covalent bonds with membrane proteins

Option D: Adhesion ensures that water molecules move independently of the extracellular matrix and membranes

Option B: Adhesion via hydrogen bonding to polar groups maintains hydration and proper membrane function

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Bacteria, insects, and plants use carbohydrates to build structures. Which of the following is TRUE of structural carbohydrates?

Option A: Different types of pentose monomers form the basis of all carbohydrate-based structures.

Option B: Structural carbohydrates show a high degree of branching.

Option C: All structural carbohydrates are made from the same monomer, α-glucose.

Option D: Structural carbohydrates are long strands, which are chemically linked into a network

Option D: Structural carbohydrates are long strands, which are chemically linked into a network

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Proteins that interact with DNA often interact with the phosphates that are part of this molecule. Which of the following types of amino acids would you predict to be present in the part of the protein that interacts with the phosphates in DNA?

Option A: Basic amino acids

Option B: Acidic amino acids

Option C: Polar amino acids

Option D: Nonpolar amino acids

Option A: Basic amino acids

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<p><span><span>A mutation occurs in this middle portion of a DNA molecule, where an adenine replaces a cytosine. What impact do you think this will have on the DNA structure?</span></span></p><p>Option A: Adenine is larger than cytosine and will not be able to base pair properly with the guanine on the opposing strand. This will cause the phosphodiester bonds to break, and the DNA will hydrolyze.</p><p>Option B: Adenine is larger than cytosine and will not be able to base pair properly with the guanine on the opposing strand. This will cause the DNA to denature or unzip.</p><p>Option C: Adenine is larger than cytosine and will not be able to base pair properly with the guanine on the opposing strand. This will cause the DNA to bulge.</p><p>Option D: Since adenine is still a nitrogenous base, this mutation will have no impact on the DNA structure</p>

A mutation occurs in this middle portion of a DNA molecule, where an adenine replaces a cytosine. What impact do you think this will have on the DNA structure?

Option A: Adenine is larger than cytosine and will not be able to base pair properly with the guanine on the opposing strand. This will cause the phosphodiester bonds to break, and the DNA will hydrolyze.

Option B: Adenine is larger than cytosine and will not be able to base pair properly with the guanine on the opposing strand. This will cause the DNA to denature or unzip.

Option C: Adenine is larger than cytosine and will not be able to base pair properly with the guanine on the opposing strand. This will cause the DNA to bulge.

Option D: Since adenine is still a nitrogenous base, this mutation will have no impact on the DNA structure

Option C: Adenine is larger than cytosine and will not be able to base pair properly with the guanine on the opposing strand. This will cause the DNA to bulge.

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Small nuclear ribonucleoproteins (snRNPs) play a key role in RNA splicing by catalyzing the cutting of RNA molecules. Which bonds are being broken during this process?

Option A: Peptide bonds between nucleotides

Option B: Phosphodiester linkages between amino acids

Option C: Disulfide bonds between amino acids

Option D: Phosphodiester linkages between nucleotides

Option D: Phosphodiester linkages between nucleotides

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<p><span><span>Gel electrophoresis is a technique that can be used to separate Your Favorite Protein from other proteins. The gel contains pores of different sizes allowing different molecules to pass through at different rates. Electrodes are attached so that the top end of the gel shown has a negative charge, and the bottom edge has a positive charge. After a period of time, the electrodes are removed, the gel is stained, and the location of the sample is visualized. In the gel below, a purified sample of Your Favorite Protein is in lane B. What can you conclude about Your Favorite Protein from this result?</span></span></p><p>Option A: Your Favorite Protein has quaternary structure.</p><p>Option B: Your Favorite Protein is a heterodimer.</p><p>Option C: Your Favorite Protein has an α-helix and a β-pleated sheet.</p><p>Option D: All of the above</p><p></p>

Gel electrophoresis is a technique that can be used to separate Your Favorite Protein from other proteins. The gel contains pores of different sizes allowing different molecules to pass through at different rates. Electrodes are attached so that the top end of the gel shown has a negative charge, and the bottom edge has a positive charge. After a period of time, the electrodes are removed, the gel is stained, and the location of the sample is visualized. In the gel below, a purified sample of Your Favorite Protein is in lane B. What can you conclude about Your Favorite Protein from this result?

Option A: Your Favorite Protein has quaternary structure.

Option B: Your Favorite Protein is a heterodimer.

Option C: Your Favorite Protein has an α-helix and a β-pleated sheet.

Option D: All of the above

Option A: Your Favorite Protein has quaternary structure.

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The molecular chaperone Hsp70 normally contains a valine in its binding pocket, allowing it to interact with regions of newly synthesized proteins. Suppose a mutation changes this valine to aspartic acid. Predict what might happen to the folding of a protein’s alpha and beta subunits.

Option A: The protein’s alpha and beta subunits would fold properly

Option B: The protein’s alpha and beta subunits would fold properly but the tertiary structure would aggregate and misfold

Option C: The protein would remain in a linear, unfolded state

Option D: The protein would be unable to fold properly and aggregate

Option D: The protein would be unable to fold properly and aggregate

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In the following reaction, the fatty acid palmitic acid (CH3(CH2)14COOH) is broken down. In this _____________ reaction, _____________ is oxidized and _____________ is reduced.

CH3(CH2)14COOH + NAD+ → CH3(CH2)12 COOH + NADH + H+

Option A: Exergonic; Palmitic Acid; NAD+

Option B: Exergonic; NADH; Palmitic Acid

Option C: Endergonic; NAD+; Palmitic Acid

Option D: Endergonic; Palmitic acid; NADH

Option A: Exergonic; Palmitic Acid; NAD+

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Patients with Gaucher’s Disease have a mutation in the beta-glucosidase (GBA) gene, which leads to a deficiency in the lysosomal enzyme glucocerebrosidase. Which of the following does glucocerebrosidase require to be correctly delivered to the lysosome? Select all that apply.

Option A: Nuclear localization signal (NLS)

Option B: Nuclear export signal (NES)

Option C: Mannose-6-phosphate tag (M6P)

Option D: Endoplasmic reticulum signal sequence (ERSS)

Option E: Glycosylation in the rough ER

Option C: Mannose-6-phosphate tag (M6P)

Option D: Endoplasmic reticulum signal sequence (ERSS)

Option E: Glycosylation in the rough ER

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Enzyme activity refers to the rate at which a reaction is catalyzed, whereas enzyme regulation is when, where, and how a reaction occurs. Which of the following best distinguishes between enzymatic activity and regulation?

Option A: Enzymatic activity is influenced by factors such as temperature and pH, while enzyme regulation depends on the binding of an allosteric activator

Option B: Enzymatic activity is controlled only by substrates, while enzyme regulation is controlled only by cofactors

Option C: Enzymatic activity depends only on the presence of inhibitors, while enzyme regulation depends on environmental factors such as temperature and pH

Option D: Enzymatic activity is constant, while enzyme regulation is temporary

Option A: Enzymatic activity is influenced by factors such as temperature and pH, while enzyme regulation depends on the binding of an allosteric activator

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How might a change of one amino acid at a site, distant from the active site of an enzyme, alter an enzyme's substrate specificity?

Option A: By changing the enzyme's location in the cell

Option B: By changing the enzyme's stability

Option C: By changing the overall shape of an enzyme

Option D: By changing the enzyme's optimum pH

Option C: By changing the overall shape of an enzyme

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Which of the following correctly matches a component of the cytoskeleton to one of its functions?

Option A: Microtubules carry vesicles across the cell

Option B: Microfilaments aid animal cells in cell division

Optin C: Intermediate filaments cause bending of cilia

Option D: Actin filaments anchor the nucleus

Option A: Microtubules carry vesicles across the cell

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Axonemal dyneins are responsible for the movement of cilia and flagella, whereas cytoplasmic dynein facilitates movement of organelles and other cargo, just like kinesin. These motor proteins, dynein and kinesin, walk on microtubules carrying their cargo. If a cell receives a molecule by endocytosis, which motor protein would deliver the vesicle to its destination in the cell and how?

Option A: Kinesin walking towards the plus end of the microtubule

Option B: Kinesin walking towards the minus end of the microtubule

Option C: Dynein walking towards the plus end of the microtubule

Option D: Dynein walking towards the minus end of the microtubule

Option D: Dynein walking towards the minus end of the microtubule

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Hexokinase binds to glucose and catalyzes a chemical reaction that changes the glucose to glucose 6-phosphate. Once this reaction occurs, hexokinase _____.

Option A: has been converted to glucose 6-phosphate

Option B: is the product of the reaction

Option C: cannot be used again

Option D: is unchanged

Option D: is unchanged

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In a series of enzyme catalyzed reactions of A + B → C → D → E, we find that increasing the concentration of A & B to 10 M steadily increases the rate of production of E. When A & B are raised to 11 M or more, the rate of production of C continues to rise, but the rate of production of D & E does not change. What might be occurring?

Option A: Saturation of the enzyme that catalyzes C → D.

Option B: Competitive inhibition of the enzyme that catalyzes A + B → C.

Option C: Feedback inhibition of E on the enzyme that catalyzes A + B → C.

Option D: Competitive inhibition of the enzyme that catalyzes D → E.

Option A: Saturation of the enzyme that catalyzes C → D.

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In the series of enzyme catalyzed reactions of A + B → C → D → E, you learn that the enzyme that catalyzes the A + B → C reaction has three known inhibitors: Inhibitor 1, Inhibitor 2, and Inhibitor 3. You observe the following:

  1. Inhibitor 1 binds reversibly to the enzyme. Its inhibitory effect can be overcome by increasing the concentration of its substrate.

  2. Inhibitor 2 binds at a site other than the active site to decrease enzyme activity. However, its inhibition cannot be overcome by increasing the concentration of its substrate.

  3. Inhibitor 3, which is structurally distinct from the enzyme’s substrate, only accumulates when high levels of product E are produced. Inhibitor C binds to the same site as Inhibitor 2 to reduce enzyme activity.

How would you classify Inhibitor 1 and Inhibitor 3, and how would you describe the regulation of this metabolic pathway?

Option A: 1: Allosteric inhibitor; 3: Allosteric inhibitor; Regulation: Feedback inhibition

Option B: 1: Competitive inhibitor 3: Feedback inhibitor; Regulation: Allosteric inhibition

Option C: 1: Competitive inhibitor 3: Allosteric inhibitor; Regulation: Feedback inhibition

Option D: 1: Allosteric inhibitor; 3: Competitive inhibitor; Regulation: Competitive inhibition

Option E: 1: Competitive inhibitor; 3: Feedback inhibitor; Regulation: Feedback inhibition

Option C: 1: Competitive inhibitor 3: Allosteric inhibitor; Regulation: Feedback inhibition

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A solution of starch at room temperature does NOT readily decompose to form a solution of simple sugars because __________ .

Option A: the starch solution has less free energy than the sugar solution

Option B: the activation energy barrier for this reaction cannot easily be surmounted at room temperature

Option C: the hydrolysis of starch to sugar is endergonic

Option D: starch cannot be hydrolyzed in the presence of so much water

Option B: the activation energy barrier for this reaction cannot easily be surmounted at room temperature

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In a synthesis reaction, glucose and fructose are combined to make sucrose. Under standard conditions, the ΔG of this uncoupled reaction is +27 kJ/mol. Predict how introducing ATP will affect this synthesis reaction.

Option A: The uncoupled reaction is endergonic. Coupling the reaction with ATP hydrolysis lowers the enzyme’s activation energy, but the reaction is still endergonic.

Option B: The uncoupled reaction is endergonic. Coupling the reaction with ATP hydrolysis divides the reaction into two steps, both of which are exergonic.

Option C: The uncoupled reaction is exergonic. Coupling the reaction with ATP hydrolysis lowers the enzyme’s activation energy, and the reaction becomes endergonic.

Option D: The uncoupled reaction is exergonic. Coupling the reaction with ATP hydrolysis divides the reaction into two steps, both of which are endergonic.

Option E: Adding ATP does not affect the reaction.

Option B: The uncoupled reaction is endergonic. Coupling the reaction with ATP hydrolysis divides the reaction into two steps, both of which are exergonic.

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Recall the "Ticket to Nowhere" case study where you were a student intern under the mentorship of Dr. Crumpler. Maria and Francisco Silva were so happy with the arrival of their new baby girl, Ana Sophia. By the time she was six months old, however, her parents had some concerns about her growth and development. Dr. Crumpler feared that Ana Sophia may have a rare disorder. She invited you to investigate the most common Lysosomal Storage Disorders and their underlying causes. While conducting your research and working on Ana Sophia's case, which of the following statements did you find to be true (select all that apply):

Option A: Ana Sophia's lysosomal hydrolases were found in the lysosomes of her cells, but they were nonfunctional because the pH in the lysosome was too acidic.

Option B: Ana Sophia's lysosomal hydrolases were found outside her cells in the blood, and they were functional when tested at an acidic pH.

Option C: It is possible that Ana Sophia's lysosomal hydrolases were synthesized without an ER signal sequence and left the cell by facilitated diffusion.

Option D: It is possible that Ana Sophia's lysosomal hydrolases were properly tagged with mannose-6-phosphate in the Golgi and were delivered to the lysosome.

Option E: It is possible that Ana Sophia's lysosomal hydrolases were not properly tagged with mannose-6-phosphate in the Golgi and were delivered to the plasma membrane for exocytosis.

Option F: Ana Sophia was ultimately diagnosed with a lysosomal storage disorder called Gaucher's Disease.

Option B: Ana Sophia's lysosomal hydrolases were found outside her cells in the blood, and they were functional when tested at an acidic pH.

Option E: It is possible that Ana Sophia's lysosomal hydrolases were not properly tagged with mannose-6-phosphate in the Golgi and were delivered to the plasma membrane for exocytosis.

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You have introduced a mutation in the electron carrier plastocyanin (PC) so it can no longer accept electrons from plastoquinone (PQ) in the cytochrome complex. Predict how this mutation would immediately affect production of ATP and NADPH.

Option A: a. Both ATP and NADPH production stop completely    

Option B: b. ATP production continues at normal levels, but NADPH production stops

Option C: c. ATP production decreases, but NADPH production stops completely.

Option D: d. Both ATP and NADPH production decrease, but neither stops completely

Option E: e. ATP production stops completely, but NADPH production continues at normal levels.

Option C: c. ATP production decreases, but NADPH production stops completely.

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A signaling pathway is observed in a tissue where:

  • Signal transmission occurs between adjacent cells

  • The receptor is embedded in the plasma membrane

  • Signal transduction occurs without the activation of a phosphorylation cascade

  • The signal results in a change in cellular gene expression

Which of the following signaling pathways does this description most accurately describe?

Option A: a. Lipid soluble signaling

Option B: b. Notch signaling

Option C: c. G-protein coupled receptor signaling

Option D:d. Enzyme-linked receptor signaling

Option B: b. Notch signaling

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Assume the outer mitochondrial membrane is punctured so that the mitochondrial intermembrane space is no longer separated from the cytosol. This damage will most directly affect the ________.

Option A: a. ATP synthesis through substrate-level phosphorylation

Option B: b. The process of glycolysis

Option C: c. The process of fermentation

Option D: d. ATP synthesis through oxidative phosphorylation

Option D: d. ATP synthesis through oxidative phosphorylation

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<p><span><span>What if NADH competitive inhibition occurs, what will happen to the levels of malate and isocitrate in the citric acid cycle shown in the accompanying figure?</span></span></p><p>Option A: a. Malate will decrease, and isocitrate will accumulate</p><p>Option B: b. Both malate and isocitrate will decrease</p><p>Option C: <span style="background-color: transparent; font-family: Arial, sans-serif, Inter, ui-sans-serif, system-ui, -apple-system, BlinkMacSystemFont, &quot;Segoe UI&quot;, Roboto, &quot;Helvetica Neue&quot;, &quot;Noto Sans&quot;, &quot;Apple Color Emoji&quot;, &quot;Segoe UI Emoji&quot;, &quot;Segoe UI Symbol&quot;, &quot;Noto Color Emoji&quot;; font-size: 1.6rem;"><span>c. Malate will accumulate, and isocitrate will decrease</span></span></p><p>Option D: d. Both malate and isocitrate will accumulate</p><p>Option E: e. Malate will remain constant, and isocitrate will increase</p>

What if NADH competitive inhibition occurs, what will happen to the levels of malate and isocitrate in the citric acid cycle shown in the accompanying figure?

Option A: a. Malate will decrease, and isocitrate will accumulate

Option B: b. Both malate and isocitrate will decrease

Option C: c. Malate will accumulate, and isocitrate will decrease

Option D: d. Both malate and isocitrate will accumulate

Option E: e. Malate will remain constant, and isocitrate will increase

Option A: a. Malate will decrease, and isocitrate will accumulate

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Reactions that require CO2 take place in ________.

Option A: a. Both the light reactions and the Calvin cycle

Option B: b. The Calvin cycle only

Option C: c. The C4 cycle only

Option D: d. Both the Calvin cycle and the C4 cycle.

Option D: d. Both the Calvin cycle and the C4 cycle.

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What would happen if all of the cells in a developing frog embryo expressed the same type of cadherin on their surfaces?

Option A: Cells would easily dissociate from each other as the wrong cadherins would bind to each other.

Option B: All the channels between cells would be blocked, so there would be no cell-to-cell communication.

Option C: Antibodies would not be able to bind to cadherin as all the receptors would be blocked.

Option D: All of the cells in the embryo would be capable of attaching to one another regardless of the cell type.

Option D: All of the cells in the embryo would be capable of attaching to one another regardless of the cell type.

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Normal breast cells have HER2 receptors in their plasma membrane. The epidermal growth factor is the signal molecule that binds to this receptor. What if a normal breast cell develops a mutation in protein kinase 2 (aka MEK) that prevents the active form of MEK from being recognized by phosphatases. What effect would this have on the cell?

Option A: Since MEK contains a phosphate, it is unable to transduce the signal and the cell would not grow and divide.

Option B: Since MEK contains a phosphate, it is able to transduce the signal continuously and the cell would continue to grow and divide, possibly leading to cancer.

Option C: Since the phosphatase removes the phosphate from MEK, this will stop the signaling and the cell would stop growing.

Option D: Since the phosphatase cannot remove the phosphate from MEK, this will stop the signaling and the cell would stop growing.

Option B: Since MEK contains a phosphate, it is able to transduce the signal continuously and the cell would continue to grow and divide, possibly leading to cancer.

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When electrons flow along the electron transport chains of mitochondria, which of the following changes occurs?

Option A: The pH of the matrix decreases

Option B: ATP synthase pumps protons by active transport

Option C: NADH is reduced.

Option D: The pH of the intermembrane space decreases

Option D: The pH of the intermembrane space decreases

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<p><span><span>What wavelength of light in the accompanying figure is most effective in driving photosynthesis?</span></span></p><p>Option A: 625 mm</p><p>Option B: 575 mm</p><p>Option C: 420 mm</p><p>Option D: 475 mm</p>

What wavelength of light in the accompanying figure is most effective in driving photosynthesis?

Option A: 625 mm

Option B: 575 mm

Option C: 420 mm

Option D: 475 mm

Option C: 420 mm

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Lipid-soluble signaling molecules, such as cortisol, cross the membranes of all cells but affect only target cells because ________.

Option A: G-protein-coupled receptors are present only in target cells

Option B: intracellular receptors are present only in target cells

Option C: only target cells produce second messengers in response to cortisol

Option D: only target cells posses the cytosolic enzymes that transduce the cortisol

Option B: intracellular receptors are present only in target cells

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CAM plants keep stomata closed in the daytime, thus reducing loss of water. They can do this because they ________.

Option A: Fix CO2 into organic acids during the night

Option B: Fix CO2 into sugars in the bundle-sheath cells

Option C: Fix CO2 into pyruvate in the mesophyll cells

Option D: Use the enzyme PEP carboxylase, which outcompetes Rubisco for CO2 in the bundle sheath cells.

Option A: Fix CO2 into organic acids during the night

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Which of the following would halt (stop) glycolysis:

Option A: Lack of O2

Option B: Lack of CO2

Option C: Lack of NAD+

Option D: Both A and C

Option C: Lack of NAD+

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<p><span><span>Questions #1-3 refer to the following pedigree. This pedigree illustrates the inheritance pattern of two unrelated traits, Trait Blue and Trait Red, across four generations of one family. Trait Blue is one phenotype of the protein encoded by Gene A, while Trait Red is one phenotype of the protein encoded by Gene B. In this pedigree, squares represent males and circles represent females. Solid-colored shapes represent an individual who exhibits a trait and dashed lines represent an individual who does not exhibit the trait. Trait Blue is depicted on the left side of each shape while Trait Red is depicted on the right side of each shape.</span></span></p><p><span><span>What type of inheritance pattern does Trait Blue </span></span><em>most likely </em><span><span>follow?</span></span></p><p>Option A: Autosomal dominant</p><p>Option B: Autosomal recessive</p><p>Option C: Sex-linked dominant</p><p>Option D: Sex-linked recessive</p>

Questions #1-3 refer to the following pedigree. This pedigree illustrates the inheritance pattern of two unrelated traits, Trait Blue and Trait Red, across four generations of one family. Trait Blue is one phenotype of the protein encoded by Gene A, while Trait Red is one phenotype of the protein encoded by Gene B. In this pedigree, squares represent males and circles represent females. Solid-colored shapes represent an individual who exhibits a trait and dashed lines represent an individual who does not exhibit the trait. Trait Blue is depicted on the left side of each shape while Trait Red is depicted on the right side of each shape.

What type of inheritance pattern does Trait Blue most likely follow?

Option A: Autosomal dominant

Option B: Autosomal recessive

Option C: Sex-linked dominant

Option D: Sex-linked recessive

Option B: Autosomal recessive

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<p><span><span>Questions #1-3 refer to the following pedigree. This pedigree illustrates the inheritance pattern of two unrelated traits, Trait Blue and Trait Red, across four generations of one family. Trait Blue is one phenotype of the protein encoded by Gene A, while Trait Red is one phenotype of the protein encoded by Gene B. In this pedigree, squares represent males and circles represent females. Solid-colored shapes represent an individual who exhibits a trait and dashed lines represent an individual who does not exhibit the trait. Trait Blue is depicted on the left side of each shape while Trait Red is depicted on the right side of each shape.</span></span></p><p><span><span>What type of inheritance pattern does Trait Red </span></span><em>most likely </em><span><span>follow?</span></span></p><p><span><span>Option A: </span></span>Autosomal dominant</p><p>Option B: Autosomal recessive</p><p>Option C: Sex-linked dominant</p><p><span><span>Option D: </span></span>Sex-linked recessive</p>

Questions #1-3 refer to the following pedigree. This pedigree illustrates the inheritance pattern of two unrelated traits, Trait Blue and Trait Red, across four generations of one family. Trait Blue is one phenotype of the protein encoded by Gene A, while Trait Red is one phenotype of the protein encoded by Gene B. In this pedigree, squares represent males and circles represent females. Solid-colored shapes represent an individual who exhibits a trait and dashed lines represent an individual who does not exhibit the trait. Trait Blue is depicted on the left side of each shape while Trait Red is depicted on the right side of each shape.

What type of inheritance pattern does Trait Red most likely follow?

Option A: Autosomal dominant

Option B: Autosomal recessive

Option C: Sex-linked dominant

Option D: Sex-linked recessive

Option C: Sex-linked dominant

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<p><span><span>Questions #1-3 refer to the following pedigree. This pedigree illustrates the inheritance pattern of two unrelated traits, Trait Blue and Trait Red, across four generations of one family. Trait Blue is one phenotype of the protein encoded by Gene A, while Trait Red is one phenotype of the protein encoded by Gene B. In this pedigree, squares represent males and circles represent females. Solid-colored shapes represent an individual who exhibits a trait and dashed lines represent an individual who does not exhibit the trait. Trait Blue is depicted on the left side of each shape while Trait Red is depicted on the right side of each shape.</span></span></p><p><span><span>Assuming that individual 3E has a heterozygous genotype for both genes 1 and 2, what is the likelihood that individual 4D will exhibit both Trait Blue and Trait Red?</span></span></p><p>Option A: 0%</p><p>Option B:12.5%</p><p>Option C: 25%</p><p>Option D: 37.5%</p><p>Option E: 50%</p><p>Option F: 62.5%</p><p>Option G: 75%</p><p>Option H: 87.5%</p><p>Option I:100%</p>

Questions #1-3 refer to the following pedigree. This pedigree illustrates the inheritance pattern of two unrelated traits, Trait Blue and Trait Red, across four generations of one family. Trait Blue is one phenotype of the protein encoded by Gene A, while Trait Red is one phenotype of the protein encoded by Gene B. In this pedigree, squares represent males and circles represent females. Solid-colored shapes represent an individual who exhibits a trait and dashed lines represent an individual who does not exhibit the trait. Trait Blue is depicted on the left side of each shape while Trait Red is depicted on the right side of each shape.

Assuming that individual 3E has a heterozygous genotype for both genes 1 and 2, what is the likelihood that individual 4D will exhibit both Trait Blue and Trait Red?

Option A: 0%

Option B:12.5%

Option C: 25%

Option D: 37.5%

Option E: 50%

Option F: 62.5%

Option G: 75%

Option H: 87.5%

Option I:100%

Option C: 25%

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<p>Based on the following mRNA codon sequence, what amino acid sequence will be generated?</p><p>5’-ACUGAUGACCCAUGGCGUGUGAGCU-3’</p><p>Option A: Thr-Asp-Asp-Pro-Trp-Arg-Val-Ser</p><p>Option B: Ser-Ser-Val-Arg-His-Pro-Val-Val</p><p>Option C: Met-Ala-Cys-Glu-Pro</p><p>Option D: Met-Thr-His-Gly-Val</p>

Based on the following mRNA codon sequence, what amino acid sequence will be generated?

5’-ACUGAUGACCCAUGGCGUGUGAGCU-3’

Option A: Thr-Asp-Asp-Pro-Trp-Arg-Val-Ser

Option B: Ser-Ser-Val-Arg-His-Pro-Val-Val

Option C: Met-Ala-Cys-Glu-Pro

Option D: Met-Thr-His-Gly-Val

Option D: Met-Thr-His-Gly-Val