Carb metabolism- TCA cycle

what continues the catabolism of glucose

pyruvate metabolism and the TCA cycle

what does pyruvate metabolism complete

completes the oxidation of pyruvate made from glycolysis to CO2

what does the TCA cycle produce

NADH and FADH2

where is pyruvate metabolism and the TCA cycle

in all cells that respire/ use oxygen during catabolism

how many steps is pyruvate metabolism

a single step with two products

what does pyruvate metabolism connect

glycolysis with the TCA cycle

what are the two important products

Acetyl CoA and NADH

what is pyruvate oxidized into

acetyl coA

what is acetyl CoA

the center of metabolism

what is acetyl CoA catabolized through

TCA cycle but is also used for anabolism

how many steps does the TCA cycle have

8

what products does the TCA cycle produce

3 NADH, 1 FADH2, 1 GTP

what is the goal of the TCA cycle

oxidize 1 acetyl CoA into 2 CO2

what is the goal of pyruvate metabolism and TCA cycle

made NADH and FADH2 to be used to make ATP in oxidative phosphorylation

what occurs in step 1 of the TCA cycle

condensation of acetyl coA (2 carbons) with oxaloacetate (4 carbons) and product→ citrate (6 carbons)

what is step 2 of the TCA cycle

isomerization of citrate to isocitrate→ intermediate cisaconitate

what is step 3 of the TCA cycle

oxidation and decarboxylation of isocitrate→ forms NADH or NADPH→ product is alpha ketoglutarate (5 carbons)

what is step 4 of the TCA cycle

oxidation and decarboxylation of alpha ketoglutarate→ forms NADH→ product is succinyl CoA (4 carbons)

what is step 5 of the TCA cycle

conversion of succinyl-CA → formation of GTP/ATP→ product is succinate

what the only step of the TCA cycle that directly forms NTP

step 5 → rest are formed during oxidative phosphorylation

what occurs in step 6 of the TCA cycle

oxidation of succinate→ forms FADH2 that is bound to the enzyme

what occurs in step 7 of the TCA cycle

hydration of fumarate→ product is L malate

what occurs in step 8 of the TCA cycle

oxidation of malate→ formation of NADH→product is oxoacetate

what is the substrate for the first step of the TCA cycle

oxaloacetate

what is the product of TCA step 1

citrate; acetyl CoA→ citrate

what is the product of TCA step 2

isocitrate; citrate→ isocitrate

what is the product of TCA step 3

alpha ketoglutarate; isocitrate→ alpha ketoglutarate

what is the product of TCA step 4

succinyl-CoA; alpha ketoglutarate→ succinyl-CoA

what is the product of TCA step 5

succinate; succinyl-coA→ succinate

what is the product of TCA step 6

fumarate; succinate→ fumarate

what is the product of TCA step 7

malate; fumarate→malate

what is the product of TCA step 8

oxaloacetate; malate→ oxalacetate

what steps are NADH produced

step 3, step 4, step 8

what step is GTP and FADH2 produced

4 (GTP) and 5 (FADH2)

how many reduced co-enzymes are produced in the TCA cycle

6 NADH and 2 FADH2

how many reduced enzymes per glucose are made in glycolysis

2 NADH

how many reduced enzymes per glucose are made in pyruvate decarboxylation

2 NADH

what is the fate of reduced co-enzymes from TCA cycle

oxidative phosphorylation or fermentation

what other roles does TCA cycle serve in other than glucose catabolism

catabolism→ of amino acids, fatty acids, anything else making acetyl- CoA

anabolism→ intermediates are put into anabolic pathways