lab 4 dna purification and rflp

what pcr product was used in this lab?

pcr b

2 restriction enzymes used in rflp?

RsaI and MesI

what 3 thinfs wre placed in the tubes to prepare for rflp?

purified pcr, cutsmart and restriction enzyme

function of restriction enzymes?

cuts dna as specific resitricytion sites that are palindromic, control region

What are polymorphisms? How is it useful with RFLP gel electrophoresis?

individuals in a population who possess multiple single nucleotide differences in genome \n \n cutting out DNA from different organisms could yield potentially different lengths

what colum was used?

Qia quick

Buffer used in this lab?

PB, PE, EB

Buffer PB?

cehmical dentaturing agent, salts to denature protiens

Buffer PE?

washing agent, salts and 70% ethanol

Buffer EB?

elution buffer, allowas dna to relase and flow through the column