BACTE 5***

If possible specimens should be collected in what phase of infection before taking antibiotics

Acute/prodromal phase

T/F

A written order from a nurse is required before processing bacte culture

False

Must be by physician

T/F

Collected infectimg agents with the presence of normal flora is acceptable

False?

Not sure but it must be avoided

T/F

Aspiration from an open wound is sueprior to unopen wounds

False

Unopen > open

What is usually used for aspirates & tissues

Needle & syringe

Used for specimens with upper respiratory tract infection

Swabs

Used as last resort for wound specimen

Swab

What swabs are preferred over cotton & calcium alginate

Dacron/polyester & rayon swabs

Collection method of urine for bacte tests

Midstream

A patient submitted a sputum sample to the lab, they said that they removed dentures, rinsed their mouth, & gargled with listerine. The lab did not accept the specimen, why

Gargle only with water

Method used to collect specimen with deep cough

Expectorate

Best collection method for sputum/lower respiratory tract specimen

First morning

If expectoration is impossible what is an alternative

Aspiration

T/F

Sterile containers for stool are unecessary

True

The following are major concerns in storage & transport EXCEPT:

Safety of transporter

Cell death due to temp & ph change

Una from oxygen for aerobes

Overgrowth

NOTA

Protection from oxygen for aerobes

For unaerobes

What are the preservatives for urine & stool

Urine - boric acid

Stool - phosphate buffered saline

The most common anticoagulant used for bacte

Sodium polyanethol sulfonate (SPS)

Anticoagulant for viral cultures

Heparin

1 citrate can be used for bacte as anticoagulant

2 EDTA can be used for bacte as anticoagulant

1 is true 2 is false

1 is false 2 is true

Both are false

Both are true

Both are false

Citrate & EDTA must not be used for bacte

Carey-Blair

Modified stuart

Amies

Are all examples of

Holding & transport media

T/F

Unprotected bacte specimen can be processed up to 3 hrs

False

Process without delay until 2 hrs only

The following can be refrigerated at 4C EXCEPT:

Urine

CSF

Viral blood

Catheter

Swabs

NOTA

CSF

It is incubated

The following can be incubated at 4C EXCEPT:

CSF

Bacte blood spn

Agar plate culture

AOTA

NOTA

AOTA

Incubate at 37C

How are spn for isolation of fungus stored

room temp 25C

how should spn that will be delayed for more than 4 days be stored

freezing (-20C or -70C)

the following should be labeled in the spn EXCEPT:

px name/ID #

date & time of collection

site of collection

phleb initial/name

px birthday

NOTA

birthday

the following should be indicated in requisitions EXCEPT:

px name/ID #

px age & sex

site of collection

initial diagnosis/history

test requested

Dr name

date & time collection

room #

NOTA

NOTA

T/F

age & sex of px will make it easier to indentify pathogens

true