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How are cellular processes powered?
Energy released from chemical reactions, mainly through ATP and energy coupling between exergonic and endergonic reactions.
First law of thermodynamics
Energy cannot be created or destroyed, only transferred or transformed.
Second law of thermodynamics
Every energy transfer increases entropy (disorder) in the universe.
How is order (entropy) maintained in a system?
By a constant input of energy that offsets the natural tendency toward disorder.
How are cellular processes powered (ATP focus)?
By exergonic reactions that release energy, often coupled to ATP hydrolysis.
Exergonic reaction
A reaction that releases energy and occurs spontaneously (ΔG < 0).
Endergonic reaction
A reaction that requires energy input and is not spontaneous (ΔG > 0).
Energy coupling
Using energy from exergonic reactions (like ATP hydrolysis) to drive endergonic reactions.
Why is energy coupling needed in cells?
Many essential cellular reactions are endergonic and need energy input.
What happens when energy decreases or order is lost?
Cellular processes fail, homeostasis is lost, and the organism may die.
Metabolic pathway
A series of enzyme-catalyzed reactions where each product becomes the next reactant.
Catabolic vs anabolic pathways
Catabolic pathways break down molecules and release energy; anabolic pathways build molecules and require energy.
Relationship between steps in a metabolic pathway
The product of one step is the reactant of the next.
Effect of decreased tryptophan
Decreased IAA production.
Effect of decreased enzyme Trp-T
Decreased I3PA and IAA production.
Effect of decreased I3PA
Decreased IAA production.
Effect of decreased enzyme YUC
Decreased IAA production.
Effect of decreased IAA
Reduced final product; pathway output decreases.
All organisms perform glycolysis (True/False)
TRUE
Conservation of glycolysis
Occurs in Archaea, Bacteria, and Eukarya, showing it evolved early and is essential.
Why glycolysis evolved early
Does not require oxygen or membrane-bound organelles.
Oxidative phosphorylation in archaea
Occurs across the plasma membrane using an ETC and proton gradient.
Oxidative phosphorylation in bacteria
Occurs across the plasma membrane using an ETC and ATP synthase.
Oxidative phosphorylation in eukaryotes
Occurs in the inner mitochondrial membrane.
Photosynthesis
Using light energy to convert CO₂ and H₂O into sugars, releasing O₂.
Reactants of photosynthesis
CO₂, H₂O, and light energy.
Products of photosynthesis
Glucose and O₂.
Description of photosynthesis
Light reactions produce ATP and NADPH; the Calvin cycle uses them to make sugars.
Photosynthetic organisms
Plants, algae, and cyanobacteria.
First photosynthetic organisms
Prokaryotes (cyanobacteria).
Evidence of atmospheric oxygenation
Banded iron formations.
Origin of eukaryotic photosynthesis
Endosymbiosis produced chloroplasts.
Chloroplast function
Captures light energy to produce sugars.
Stroma
Fluid-filled interior of the chloroplast where the Calvin cycle occurs.
Thylakoid
Membrane sacs containing chlorophyll.
Grana
Stacks of thylakoids.
Thylakoid membrane function
Site of light reactions, ETC, and ATP synthase.
Thylakoid space (lumen)
Region of proton accumulation.
Chlorophyll
A pigment that absorbs light energy.
Location of chlorophyll
Thylakoid membrane.
Light reactions
Light excites electrons, ETC produces ATP and NADPH, and water is split to release O₂.
Calvin cycle
Uses ATP and NADPH to fix CO₂ into sugars.
Connection between light reactions and Calvin cycle
ATP and NADPH from light reactions power carbon fixation.
Electron transport chain (ETC)
A series of proteins that transfer electrons and pump protons.
Location of photosynthetic ETC
Thylakoid membrane.
Linear vs cyclic electron flow
Linear produces ATP, NADPH, and O₂; cyclic produces ATP only.
Photolysis
Splitting of water at photosystem II to replace electrons.
Why photosynthesis stops without photolysis
No electrons means no ETC, ATP, or NADPH.
Chemiosmosis
ATP synthesis driven by proton movement through ATP synthase.
Photophosphorylation
ATP synthesis using light energy.
Cellular respiration
Conversion of glucose into ATP.
Fermentation
ATP production without oxygen.
Stages of cellular respiration
Glycolysis, Krebs cycle, and electron transport chain.
Final electron acceptor in aerobic respiration
Oxygen.
Final electron acceptor in anaerobic respiration
Other molecules such as nitrate.
Location of glycolysis
Cytosol.
Oxygen requirement of glycolysis
Does not require oxygen.
Substrate-level phosphorylation
Direct ATP synthesis from a phosphorylated intermediate.
Location of Krebs cycle
Mitochondrial matrix.
Oxidation
Loss of electrons.
Reduction
Gain of electrons.
Purpose of fermentation
Regenerates NAD⁺ so glycolysis can continue.
Location of fermentation
Cytosol.
Why aerobic respiration is more efficient
Produces much more ATP.