W5 L12: Translation in Eukaryotes

EIF4A

EIF4A

________ is helicase used to unwinds RNA structure to be scanned properly.

SURF complex

________ formed due to paused ribosome in proximity of EJC → adaptor to bring in RNA decay effectors that dispose of mRNA that is detected as abnormal.

Caudal gene

________ → important for patterning the posterior end of embryo, but its mRNA is expressed throughout the embryo- bicoid stops protein being where bicoid is present.

PERK

________ inhibits protein synthesis so that backlog is reduced.

HCV NS5A

________- make antagonists of kinase activity of PKR- so they directly stop activity or kinase domain.

EIF1

________ & 1A- important for locating correct AUG → to avoid frame shifts.

ORF

________ contributes to protein code being produced- section from start codon to end codon.

Msl2 mRNA

________ is transcribed in both, but translated in male but not female during development- to repress production in female flies.

Bicoid

________ (RBP) is RNA binding protein that interacts w /3 UTR of caudal mRNA.

Apoptosis

________ important in development- to get rid of cells that are no longer needed & in normal tissue homeostasis to eliminate infected cells.

GTP hydrolysis

At the end of translation, eIF2 associates w /GDP due to ________ during initiation.

PKR

________ has 1 domain that helps bind dsRNA & kinase domain at C terminus thats activated when ________ binds the dsRNA → leads to eIF2 alpha phosphorylation → blocks viral protein production.

M⁶A

________ promotes mRNA translation by multiple mechanisms.

GTP

________ required for translation initiation- associates w /initiator tRNA & the ternary complex can participate in translation.

SXL

________ is only expressed in females & is a binding protein.

eIF4F complex

In normal cells, ________ helps to recruit 43s ribosome pre- initiation complex- through interactions that eIF4G has w /eIF3.

DsRNA

________ is hallmarker for viral infection.

PERK

________ senses ER stress- responds to presence of unfolded proteins in ER.

METTL3

________ helps install m⁶A & adds on m⁶A → promotes translation of message m⁶A is on (cap- dependant translation)

Bicoid

________ recruits 4E- HP (4E homologous protein) to bing caudal cap but not initiate translation.

Caudal gene

________ is an important patterning mol. during embryogenesis.