PAG 05 - Colorimeter or Potometer
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FlashcardsWhat does a colorimeter measure?
Absorbance/transmission
The paler the solution, the lower the absorbance and higher the transmission, as more light can pass through the sample
Outline the procedure to find an unknown concentration of reducing sugar
Carry out a Benedict’s test on a range of standard solutions of reducing sugar
Using the colorimeter, measure the percentage transmission for each sample
Plot a graph of transmission against reducing sugar concentration
Carry out a Benedict’s test on the unknown solution and measure the transmission using the colorimeter
Plot the transmission on the calibration curve, and read off the x axis (reducing sugar concentration) for the concentration of the unknown solution
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4 Terms
What does a colorimeter measure?
Absorbance/transmission
The paler the solution, the lower the absorbance and higher the transmission, as more light can pass through the sample
Outline the procedure to find an unknown concentration of reducing sugar
Carry out a Benedict’s test on a range of standard solutions of reducing sugar
Using the colorimeter, measure the percentage transmission for each sample
Plot a graph of transmission against reducing sugar concentration
Carry out a Benedict’s test on the unknown solution and measure the transmission using the colorimeter
Plot the transmission on the calibration curve, and read off the x axis (reducing sugar concentration) for the concentration of the unknown solution
What indicates a higher reducing sugar concentration?
Higher absorbance, lower transmission
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