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Scientific Hypothesis
A hypothesis must be testable and falsifiable.
Independent Variable (IV)
Manipulated variable plotted on the x-axis.
Dependent Variable (DV)
Measured variable plotted on the y-axis.
Control Variables
Variables kept constant in an experiment.
Control Group
Baseline comparison group in an experiment.
Placebo
Substance that reduces bias in human trials.
Controlled Experiment
Researcher manipulates the independent variable.
Observational Study
No manipulation; shows correlation, not causation.
Good Science
Reproducibility, peer review, testability, avoids pseudoscience.
Characteristics of Life
Cells, homeostasis, metabolism, growth & reproduction, response to environment.
Levels of Organization
Atom → molecule → organelle → cell → tissue → organ → system → organism → population → community → ecosystem → biosphere.
Prokaryotes
No nucleus, circular DNA, includes bacteria and archaea.
Eukaryotes
Contains a nucleus, linear chromosomes, includes animals, plants, fungi, protists.
Mitosis
1 division resulting in 2 identical diploid cells.
Meiosis
2 divisions resulting in 4 unique haploid gametes.
Transcription
Process where DNA is converted to mRNA.
Translation
Process where mRNA is translated into amino acids.
Mutations
Changes in DNA sequence; types include silent, missense, nonsense, frameshift.
Glycolysis
Occurs in cytoplasm, splits glucose, produces 2 ATP, 2 NADH, 2 pyruvate.
Krebs Cycle
Occurs in mitochondrial matrix, produces NADH, FADH₂, ATP, CO₂.
Electron Transport Chain
Uses NADH/FADH₂ to produce ~30 ATP; O₂ is the final electron acceptor.
10% Rule
Only 10% of energy transfers between trophic levels.
Biomagnification
Toxins accumulate in fat and become more concentrated at higher trophic levels.
Eutrophication
Process triggered by fertilizer runoff leading to oxygen depletion in water bodies.
Carbon Cycle
Photosynthesis ↔ respiration.
Nitrogen Cycle
Nitrogen-fixing bacteria convert N₂ to nitrate, which is used by plants.
Phosphorus Cycle
Phosphorus comes from rocks and is often a limiting nutrient in freshwater.
Evolution
Change in allele frequencies in a population over time.
Natural Selection
Acts on phenotypes, requires variation, heritability, and differential survival/reproduction.
Speciation
Requires reproductive isolation: geographic, behavioral, or temporal.
Innate Immunity
Fast, general defense mechanisms including barriers and immune cells.
Adaptive Immunity
Specific immune response involving B cells and T cells.
Photosynthesis Overall Equation
6CO₂ + 6H₂O + light → C₆H₁₂O₆ + 6O₂.
Calvin Cycle
Occurs in stroma; uses CO₂, ATP, and NADPH to produce G3P.