PCR3 Microscopy and Staining

genetic organization between prokaryotes and eukaryotes.

Prokaryotes

circular chromosomes

genetic organization between prokaryotes and eukaryotes.

Eukaryotes

linear chromosomes

compare energy metabolism between prokaryotes and eukaryotes

Prokaryotes

cell membrane

compare energy metabolism between prokaryotes and eukaryotes

Eukaryotes

mitochondria

match in cellular organelles

ribosome

synthesize proteins

match in cellular organelles

endoplasmic reticulum

lipid synthesis

match in cellular organelles

mitochondria

produce ATP

match in cellular organelles

lysosome

break down captured food materials

match in cellular organelles

Golgi apparatus

processes protein and lipid sorted, packaged for transport

match in cellular organelles

centrosome

is the source of microtubules, a cytoskeleton

match in cellular organelles

cytoskeleton

give structure to cell and assist in the transport of materials

Differential flagella between in eukaryotes and in prokaryotes.

Made of microtubules

in eukaryotes

Differential flagella between in eukaryotes and in prokaryotes.

Provide a mechanical force for motility by beating back and forth in a wave-like motion

in eukaryotes

Differential flagella between in eukaryotes and in prokaryotes.

Provide a rotational propeller-like force for movement

in prokaryotes

flagella

long, thin protein projections

Cilia

made of shorter microtubules

Magnification

the increase in the apparent size of the specimen being observed

resolution

the ability that the two separate objects seen as two objects apart

refractional index

a measure of the light-bending ability of a substance

simple stains

methylene blue

negative stains

acidic dye such as nigrosin or india ink

Gram stain

safranin is needed to counter-stain

acid-fast stain

use dye, carbol-fuchsin to stain mycobacteria in red

spore stain

stains endospores