ASCP Recalls

FFP used for

Multiple coagulation deficiencies

Factor XI deficiency

Cryoprecipitate used for

Replace fibrinogen loss due to DIC

Massive bleeding

Dysfibrinogenemia with active bleeding

Plateletpheresis preparation steps

Light spin (remove RBC)

Heavy spin (spin down PLT and WBC)

Supernatant goes into bag for freezing (FFP)

Remaining plasma, platelets, and WBC = platelets

Irradiated blood prevents

GVHD

Also used for cancer patients, or patients who are immunocompromised

Leukocyte reduced blood prevents

Febrile nonhemolytic transfusion reactions (FNHTRs)

Blood generally kept at what temperature?

1-10C

Platelets and granulocytes kept at what temperature?

20-24C

Platelets usually kept on agitator to ensure that the platelets are continuously oxygenated

ACD, CPD, CPD2 expiration

21 days

CPDA-1 expiration

35 days

Rouleaux resolution

Saline wash and repeat

Common IgM antibodies in blood bank (Cold)

anti-I, H, M, N, P1, Lea, Leb

Common IgG antibodies in blood bank (Warm)

anti-D, C, c, E, e, M (some), K, k, Fya, Fyb, Jka, Jkb

Antibodies enhanced by enzyme panel

RhIp Lewis the Kidd

Rh, I, Lea, Leb, Jka, Jkb

Overproduction of IgM associated with

Waldenstrom's Primary Macroglobulinemia

Overproduction of IgG associated with

Multiple Myeloma

Serum calcitonin elevated in

Medullary carcinoma of thryroid

Normal myeloid:erythroid ratio

2:1 to 4:1

Centromere ANA pattern

CREST syndrome

Homogeneous or speckled ANA pattern

SLE

Speckeled ANA pattern

RA or Sjorgen's

Measurement of sodium/chloride in sweat is useful test for

Cystic Fibrosis

Analytes increased due to delay in centrifugation

AST, ALT, Potassium, Creatinine

Analytes decreased due to delay in centrifugation

Glucose, ionized calcium, bicarbonate, folate

Reducing sugars include

Lactose, glucose, ribose

Incorporation of sulfamethoxazole and trimethoprim in culture media enhance recovery of

Strep. pyogenes

Longer staining times associated with which type of smear

Bone marrow smear

Dosage associated with homozygous or heterozygous cells

Homozygous

Confirmatory test for syphilis

FTA-ABS (fluorescent treponemal antibody absorption test)

VRDL and RPR are utilize which type of methodology

Flocculation

Mycobacterium species positive for nitrate reduction

Mycobacterium tuberculosis

Measles (rubella), mumps, polio vaccine deferral time period

2 weeks

Burkitt's lymphoma

8:14 translocation; associated with EBV; "starry sky" appearance on histology

Hodgkin Lymphoma (HL)

is a malignant lymphoma

Reed Sternberg cells are the hallmark of HL. Enlarged, painless lymph node in the neck (often the first sign of HL).

A defective clot retraction might be caused by

Lack of defect of platelet receptor IIb/IIIa

German measles vaccine deferral time period

4 weeks

This protozoa can be routinely cultured

Acanthamoeba spp.

Aspirin prevent synthesis of

Thromboxane A2

Mycobacterium spp whose colonies are photochromogenic

Mycobacterium kansaii

Burkholderia cepacia

GNB that is motile with polar tufts of flagella and produces yellow pigment

Oxidase (+)

Pyocyanin (-)

Arginine dihydrolase (-)

Known to cause fulminating lung infections in CF patients

Burkholderia mallei

Glander's disease

forms nodular lesions in the lung tissue and can cause ulcers leading to sepsis and death.

Clenched fist cellulitis associated with what organism

Eikenella corrodens

CEA tumor marker

colorectal cancer

Intestinal amebiasis symptoms

Abdominal cramps

Fever

Diarrhea

Bloody stool

Type of anemia where inability to incorporate iron into protoporphyrin ring

Sideroblastic anemia

Immunoassay for hCG uses which subunit of hCG

Beta

Parasite typically comes from cat feces

Toxoplasma gondii

This condition made up of small clots made of platelets and large forms of VWF

TTP (thrombotic thrombocytopenic purpura)

Normal heme contains

Four heme and four globin chains

Detection of MRSA

Zone of <10mm with an oxacillin disk on MH agar

Molecular tests for mecA gene

Drug of choice for Clostridium difficile and MRSA

Vancomycin (glycopeptide, inhibit cell wall synthesis)

Drugs of choice for pseudomonas aeruginosa and other aerobes

Ciprofloxacin, Norfloxacin (quinolines, inhibit nucleic acid synthesis)

Drug of choice for UTI, enteric infections

Sulfamethoxazole (Sulfonomides, analogue of PABA)

Extended-spectrum beta-lactamases

Enzymes that give bacteria immunity to both penicillin and cephalosporin antibiotics

Tiny GN coccobacilli associated with wild boar

Brucella spp.

This condition is associated with oligoclonal band in CSF electrophoresis

Multiple sclerosis

Cell indicative of kidney disease

Tubular epithelial cells

Species-specific genes for MRSA

16S and 23S ribosomal RNA genes

DNA gyrase (gyrA)

Superoxide dismutase (sodA)

UA findings associated with nephrotic syndrome

Oval fat bodies

Fatty and waxy casts

Renal tubular epithelial cells

Heavy proteinuria

Foamy appearance

Follow up tests for speckled ANA patterns

Smith antibodies

SS-A/SS-b+ antibodies

Microorganism associated with tinea cruris AKA jock itch

Epidermophyton floccosum

Inactive X-chromosome in the somatic cells of mammalian females that appear as nuclear appendages

Barr body

Deferral time for terminated pregnancy in the 3rd trimester

6 weeks

Overproduced IgM associated with

Waldenstrom's disease

Common cause of atrophic rhinitis

Klebsiella ozaenae

Clinical symptoms of nephrotic syndrome

Swelling

Weight gain from fluid retention

Poor appetite

High BP

Confirmatory test for circulating lupus anticoagulant

Platelet neutralization test

This sexually transmitted parasite displays symptoms in women but men are frequently asymptomatic

4 anterior flagella and granules along its axostyle

Trichomonas vaginalis

Hypersegmented neutrophils associated with

Megaloblastic anemia which is commonly caused by vitamin B12 deficiency

Hemosiderin-laden macrophages in CSF sample associated with

Intracranial hemorrhage

Washed RBC will decrease/prevent which type of RBC transfusion reaction

Allergic reaction

Washing cells removes excess plasma proteins

This organism has dark septate hyphae, large conidia and typically contain 4 cells

Curvularia spp.

Alternaria sp.

CHAINED poroconidia HORIZONTAL and VERTICAL SEPTA

CLUB shaped bases

ADAMSTS13

Cleaves multimers of vWF from multimers into monomers

Lack of causes TTP

Deficiency is either genetic or more commonly: Produce autoantibody

Adult female with autoantibody

Atypical RBCs associated with post-splenectomy

Acanthocytes

Echinocytes

Codocytes

Spherocytes

Schistocytes

Howell-Jolly bodies

Dubin-Johnson syndrome

autosomal recessive, conj hyperbilirubinemia, defect in bili transport, black color to liver p239

Organisms known to cause bacterial vaginosis

Mobiluncus spp

Gardnerella vaginalis

Rapidly progressive fungal infection of the sinuses in immunosuppressed or diabetic patients

Phycomycosis

Neoplastic T lymphocytes that have irregular, convoluted (cerebriform) nuclear outlines

Sezary cells

This Pseudomonas spp produces pyocyanin

Pseudomonas aeruginosa

Electrolyte panel tests

Sodium

Potassium

Chloride

CO2

Hallmark of May-Hegglin anomaly

Giant platelets, large dohle body like inclusions within neutrophils, eosinophils, basophils, and monocytes

Hallmark of Pelger-Huet anomaly

Hyposegmented neutrophils (dumbell shape)

Alder-Reilly Anomaly

Inability to degrade mucopolysaccharides results in accumulation of lipids

On a slide, every leukocyte seen exhibits dense, basophilic coarse granulation

This organism is endemic in Southern and Central California

Growth of 2-21 days

Coccidioides immitis

Cause of tularemia

Francisella spp.

Poikilocytosis

Variation in shape

Anisocytosis

Variation in size

Activation unit in classical complement pathway

C4, C2, C3

Urea nitrogen RR

6-20 mg/dL

Creatinine RR

0.6-1.2 mg/dL

This malarial parasite presents reticulocytes with Schuffner's dots

Plasmodium vivax

Platelet satellitism can be result of blood collection in which type of tube

EDTA (purple top)

What chromosome is alpha chain globin coded on

Chromosome 16

What chromosome is beta chain globin coded on

Chromosome 11

Method for long-term diabetic monitoring in SS patients

Fructosamine

This organism is normal flora of skin, cause of opportunistic mycoses in immunocompromised patients

Pink pigment is hallmark characteristic

Rhodotorula spp.

Common cause of septic arthritis in patients <30y

Neisseria gonorrhoeae

This adsorption technique removes cold Ab such as anti-I

Rabbit erythrocyte stroma (RESt)

I RESt

This plasmodium spp. contains 6 to 12 merozoites

Plasmodium malariae

This biomarker is regulator of myocyte contraction

cTnT