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RNA as an
intermediate between DNA and proteins
protein synthesis occurs
outside the nucleus
RNA is synthesized in
the nucleus, but migrates to cytoplasm
DNA
deoxyribose (3’ OH only); bases: A-T, G-C, double stranded; secondary structure is double helix
RNA
ribose (2’ and 3’ OH only); bases: A-U, G-C, single stranded; secondary structure can be complex and varied
ribose makes RNA…
less stable than DNA, so more likely to be hydrolyzed
sometimes when cytosine is damaged it can become _____. This makes RNA susceptible to ________
uracil; mutation and instability
prokaryotic transcription
occurs in cytoplasm, transcription and translation are simultaneous, mRNA directly transcribed from template, RNA pol is 5 subunits, holoenzyme binds directly to promoter
eukaryotic transcription
occurs in nucleus, transcription and translation not simultaneous, pre-mRNA formed and processed to make mRNA, RNA pol is 7-10 subunits, RNA pol cannot recognize promoter alone