Fastidious Gram-Negative Rods

Pasteurella multocida reservoir

Cats, dogs

Pasteurella multocida transmission

Animal bites

Pasteurella multocida diseases

Skin and soft tissue infection, respiratory infection

Pasteurella multocida virulence

Antiphagocytic capsule

Pasteurella multocida MAC growth

No growth on MAC

Pasteurella multocida treatment

Penicillin, amoxicillin

Bordetella pertussis disease

Whooping cough

Bordetella transmission

Respiratory droplets

Bordetella pertussis virulence

Pertussis toxin, ciliostasis toxin

Bordetella best specimen

Nasopharyngeal swab from ciliated cells

Bordetella transport medium

Regan Lowe

Bordetella culture

Regan Lowe agar, Bordet Gengou agar

Bordetella pertussis MAC growth

No growth on MAC

Bordetella treatment

Macrolides

Bordetella prevention

DTaP, Tdap

Brucella reservoirs

Cattle, sheep, goats, pigs, dogs

Brucella transmission

Ingestion, inhalation, direct contact

Brucella disease

Brucellosis with fever and sweats

Brucella pathogenesis

Facultative intracellular, RES granulomas

Brucella hazards

Lab acquired infection risk

Brucella culture requirements

Three week incubation, 5 to 10 percent CO2

Brucella colony description

Small, smooth, nonhemolytic

Brucella treatment

Doxycycline plus streptomycin or rifampin

Francisella tularensis disease

Tularemia

Francisella reservoirs

Rabbits, rodents

Francisella transmission

Tick bites, animal handling, inhalation

Francisella pathogenesis

Facultative intracellular, capsule

Francisella clinical forms

Ulceroglandular, pneumonic, oculoglandular

Francisella danger

Select agent, requires BSC

Francisella culture

Cysteine enriched media

Francisella treatment

Aminoglycosides, doxycycline, fluoroquinolones

HACEK significance

Endocarditis, oral flora