ION EXCHANGE CHROMATOGRAPHY

Ion Exchange Chromatography

A technique that separates and purifies charged molecules based on their net charge.

Stationary Phase

The part of the column packed with a matrix containing charged functional groups for binding sample molecules.

Cation Exchange

A type of ion exchange chromatography where positively charged molecules bind to negatively charged stationary phase.

Anion Exchange

A type of ion exchange chromatography where negatively charged molecules bind to positively charged stationary phase.

Sample Loading

The process of applying a sample containing charged molecules to the column for separation.

Washing

The step that uses buffer solution to wash unbound molecules and impurities through the column.

Elution

The process of releasing bound molecules by changing the ionic strength or pH of the buffer.

Collecting Fractions

The collection of separated components that elute at different times based on their affinities for the stationary phase.