ION EXCHANGE CHROMATOGRAPHY

0.0(0)
studied byStudied by 0 people
learnLearn
examPractice Test
spaced repetitionSpaced Repetition
heart puzzleMatch
flashcardsFlashcards
Card Sorting

1/7

encourage image

There's no tags or description

Looks like no tags are added yet.

Study Analytics
Name
Mastery
Learn
Test
Matching
Spaced

No study sessions yet.

8 Terms

1
New cards

Ion Exchange Chromatography

A technique that separates and purifies charged molecules based on their net charge.

2
New cards

Stationary Phase

The part of the column packed with a matrix containing charged functional groups for binding sample molecules.

3
New cards

Cation Exchange

A type of ion exchange chromatography where positively charged molecules bind to negatively charged stationary phase.

4
New cards

Anion Exchange

A type of ion exchange chromatography where negatively charged molecules bind to positively charged stationary phase.

5
New cards

Sample Loading

The process of applying a sample containing charged molecules to the column for separation.

6
New cards

Washing

The step that uses buffer solution to wash unbound molecules and impurities through the column.

7
New cards

Elution

The process of releasing bound molecules by changing the ionic strength or pH of the buffer.

8
New cards

Collecting Fractions

The collection of separated components that elute at different times based on their affinities for the stationary phase.