SDS page

Loading buffer components

SDS, glycerol, Tris-HCl, bromphenol blue, DDT

SDS in loading buffer job

unfolding the protien

Glycerol in loading buffer job

allow sample to settle at bottom on the well

Tris-HCl in loading buffer do

maintain a stable pH

bromphenol blue in loading buffer job

front dye

DDT

reducing agent, breaks disulfide bonds

Stacking layer location

top of gel

Stacking layer job

organize mol via size

Stacking layer components

low polyacrylamide concentration to the crosslinked polymer

Stacking layer pH

low pH

Stacking layer pore size

large pore size

Resolving layer job

separate mol based on weight

Resolving layer components

high polyacrylamide concentration

Resolving layer pore size

small pores

Resolving layer pH

high pH

Why stain

visualize the protein

What stain was used

coomassie blue (ionic)