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What do different coloured lab signs mean?
Mandatory (blue)
Safety (green(/red))
Prohibition (red)
Hazards (yellow)
What do mandatory signs mean?
Mandatory signs are blue and that means you need to follow the specific instructions, like wearing protective clothing (e.g. lab coat), gloves or safety glasses.
What do safety signs mean?
Safety signs are either red or green. They also highlight what actions to take in case of an emergency.
Red signs indicate the potential danger associated with actions using this equipment (e.g. locations of fire alarms, fire extinguisher or fire hoses).
Green signs show what actions to take (e.g. they are highlighting exit routes, assembly points, and the location of first aid kits or telephones to call for help).
What do prohibition signs mean?
If the predominant colour of the sign is red, this means the action is prohibited in that specific area. For example, you are not allowed to eat or drink in the laboratory and you should take your lab coat off when you enter areas where people eat, like the cafeteria or the UG common room.
What do hazard signs mean?
The colour yellow indicate potential hazards you may encounter.
What are GHS symbols/pictograms?
Indicate hazards associated with chemicals
What are COSHH forms?
Control of Substances Hazardous to Health forms allow assessment of different procedures and chemicals to identify potential risks and hazards to the person performing this technique.
What are the 9 basic lab rules?
1. Wear Personal Protective Equipment (PPE).
2. No eating and drinking.
3. Do not apply cosmetics or insert contact lenses.
4. No headphones.
5. Working hours: 9 am - 5 pm. Outside these hours, students are not permitted in the labs.
6. Correct waste disposal.
7. Follow alarms.
8. Report engineering and equipment alarms to
9. Teaching Fellow, GTA or teach team. DO NOT ignore an alarm. If in doubt, ask.
Deal with contamination and spillages appropriately and seek assistance from a Teaching Fellow or GTA.
What PPE are you expected to wear?
- Tied back hair
- Goggles (especially for handling strong acids)
- Gloves
- Closed toe footwear
- Lab coat
What goes in the orange bin bags?
General lab waste
Paper towel
Gloves
Plates (96-well, cell culture, qPCR)
Empty tubes of any kind (0.2-50 mL)
Solid reagents
Gels (agarose, polyacrylamide)
Empty gel cassettes, gel combs
Cell scrapers
Wrappers (from serological pipettes, tissue culture plates)
Any other outer packaging of consumables
What goes in the sharps bin?
The sharp bins are made of hard plastic and are used to dispose of anything sharp in the labs that could cut or harm anyone (e.g. scalpels, needles, broken glass).
Please do not put anything else in these bins!
What goes in the Mini Bio Bins
Short Bio-bins are the ones you will find at your bench or inside the tissue culture cabinet. These bins are for pipette tips and cell scrapers ONLY.
Please do not dispose of anything else (e.g. gloves, tissue, tubes and serological pipettes) in these bins.
What goes in the (Long) Bio Bins
Long bio-bins are for serological pipettes or aspirator pipettes without their wrapper!
Please DO NOT push them back in their wrappers .
Dispose wrapper in the orange bin bags .
What are the two types of sinks in the lab?
- Handwash sink
- Reagents sink
What should you do in an emergency?
1. Raise the alarm (either by alerting the Teaching Fellow or Graduate Teaching Assistant (GTA) or activating the nearest fire call point).
2. Evacuate the building immediately via the nearest fire exit by following the signs.
3. Go to your assigned assemble point (in front of Burlington Danes building). Report to the Teaching Fellow, so they can ensure everyone has made it safely out of the building
4. Notify security of the fire location.
What should you not do in an emergency?
Use the lifts.
Try to tackle the fire yourself.
Take your belongings.
Call 999
What is a PEEP (Personal Emergency Egress Plan)?
Personal Emergency Egress Plans are meant for individuals with mobility issues for evacuation.
What are (mini) centrifuges for?
They can be used to spin small microcentrifuge tubes.
What is a pH meter for?
Measure the pH of solution. A very important parameter that needs to be controlled in your experiments.
What is the Nanodrop?
Spectrometer that can measure absorbance in very small volumes (~1 µL) and can measure concentration of nucleic acids (e.g. DNA, RNA).
What is the Labtech LT-4500?
Spectrometer that allows you to measure the absorbance in a multi-well plate.
What is a thermal cycler?
It can quickly heat and cool down your samples or maintain a constant temperature, useful for PCRs or enzymatic reactions.
What is a refrigerated centrifuge?
They can spin your microcentrifuge tubes and hold them at a low temperature at the same time. Crucial for sensitive samples!
What are benchtop centrifuges for?
They spin larger tubes (15 ml and 50 ml) in the lab. They are very heavy and a bit more complex to operate.
What is the CO2 incubator for?
Incubators maintain an environment that is ideal for human cells to grow, usually 37 °C, 5% CO2, humidified.
What is the iBright for?
Used to take a picture of your western blot membrane and agarose gels.
What is the QuantStudio 5 for?
qPCR machine to measure gene expression.
What is the EVOS microscope for?
Used to observe your cells and check their health status.
What is the EVOS fluorescence microscope for?
Used to observe and determine successful mammalian transfection.
What is the BC Accuri Flow cytometer for?
Used in the functional assays to measure the expression of molecules both intracellularly and on the cell surface.
Note 
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