TISSUE ADHESIVE PREPARATION

1. Mayer’s egg albumin

2. Dried albumin

3. Gelatin 1%

4. Gelatin formaldehyde mixture

5. Poly-L-lysine

6. APES (3-aminopropyltriethoxysilane)

Common Tissue Adhesive:

Adhesion

Adhesive mixtures are spread thinly on a clean, grease-free slide.

(PRINCIPLES)

Adhesive mixtures are spread thinly on a clean, grease-free slide.

Adhesion:

(PRINCIPLES)

Application

A small drop is smeared over the slide (using a finger, sponge, or another slide) and excess is removed.

(PRINCIPLES)

A small drop is smeared over the slide (using a finger, sponge, or another slide) and excess is removed.

Application:

(PRINCIPLES)

Purpose

Promotes proper adhesion of tissue sections during processing.

(PRINCIPLES)

Promotes proper adhesion of tissue sections during processing.

Purpose:

(PRINCIPLES)

• Thymol

• Glycerin

• Funnel

• Stirring rod

• Egg white

• Measuring cylinder

• Erlenmeyer flask

• Filter paper

• 50 mL bottle

Equipment And Materials:

EGG WHITE

Specimen:

25 mL of egg white

1. Measure ______ white into an Erlenmeyer flask.

(PROCEDURE)

25 mL of glycerol

2. Beat the egg white and mix in _____ thoroughly.

(PROCEDURE)

coarse filter paper

3. Filter the mixture through ______ into a clean bottle.

(PROCEDURE)

thymol

4. Add a few crystals of ____ to prevent mold growth.

(PROCEDURE)

prevent mold growth

4. Add a few crystals of thymol to _____.

(PROCEDURE)

1. Date

2. Adhesive Name

3. Group Number

5. Label the bottle with:

(PROCEDURE)

refrigerator

6. Submit the mixture for checking and store in the ____.

(PROCEDURE)

5 mL of egg white

1. Use Syringe to Measure ____ into a Beaker

(MODIFIED PROCEDURE)

5 mL of glycerol

2. Beat the egg white and mix it with ____ thoroughly.

(MODIFIED PROCEDURE)

thymol (a pinch)

3. Add a few crystals of _____ to prevent mold growth.

(MODIFIED PROCEDURE)

Drop of Tissue Adhesive

4. Add a _____ into the Slide and perform the PULL APART Technique

(MODIFIED PROCEDURE)

PULL APART Technique

4. Add a Drop of Tissue Adhesive into the Slide and perform the ____.

(MODIFIED PROCEDURE)

Floating out

6. Do the ____- of Section

(MODIFIED PROCEDURE)

PULL APART TECHNIQUE

1. Place a small drop of adhesive on a clean glass slide using a dropper

2. Position the second slide on top

3. Allow the edge of the top slide to touch the adhesive

4. Pull Apart Motion: Gently slide the top slide away from the bottom slide in a smooth, single motion.

5. Air Dry then Start Floating out

PULL APART MOTION

Gently slide the top slide away from the bottom slide in a smooth, single motion.

Mayer’s egg albumin

Is the most common choice of adhesive, because it is:

• Easy and convenient to prepare.

• Inexpensive.

• A drop of the adhesive is applied on a clean glass slide before tissues are oriented

• Easy and convenient to prepare.

• Inexpensive.

• A drop of the adhesive is applied on a clean glass slide before tissues are oriented

Mayer’s egg albumin is the most common choice because it is:

DRIED ALBUMIN

A strong adhesive

GELATIN 1%

A natural adhesive

POLY-L-LYSINE

Aqueous detergent diluted to 0.01% concentration widely used in immunohistochemistry.

3-aminopropyltriethoxysilane

APES means?

APES (3-aminopropyltriethoxysilane)

Very useful in cytology, particularly for cytospin preparations of proteinaceous or bloody material.