NEUTROPHILS

DEVELOPMENT, FUNCTION, AND KINETICS

14 DAYS

HOW MANY DAYS DOES IT TAKE FOR A MYELOBLAST TO BECOME A MATURE SEGMENTER?

1. MYELOPEROXIDASE

2. ACID-B-GLYCEROPHOSPHATASE

3. CATHEPSIN

4. DEFENSIN

5. ELASTASE

6. PROTURASE-3

ENUMERATE THE 6 PRIMARY (AZUROPHILIC GRANULES) IN A NEUTROPHIL

B2-MICROGLOBULIN, COLLAGENASE, GELATINASE, LACTOFERRIN, NEUTROPHIL GELATINASE-ASSOCIATED LIPOCALIN, TRANSCOBALAMIN 1

ENUMERATE THE 6 SECONDARY (SPECIFIC) NEUTROPHIL GRANULES

GELATINASE

COLLAGENASE

LYSOZYME

ACETYLTRANSFERASE

B2-MICROGLOBULIN

ENUMERATE THE 5 TERTIARY NEUTROPHIL GRANULES

CD11b/CD18

ALKALINE PHOSPHATASE

VESICLE-ASSOCIATED MEMBRANE-2

CD10, CD13, CD14, CD16

CYTOCHROME b558

COMPLEMENT 1q RCEPTOR

COMPLEMENT RECEPTOR-1

ENUMERATE THE 7 COMPONENTS OF SECRETORY GRANULES

PRIMARY GRANULES

GRANULES THAT ARE FORMED DURING PROMYELOCYTE STAGE?

SECONDARY GRANULES

GRANULES THAT ARE FORMED DURING MYELOCYTE AND METAMYELOCYTE STAGE?

TERTIARY GRANULES

GRANULES THAT ARE FORMED DURING METAMYELOCYTE AND BAND STAGE?

SECRETORY GRANULES

GRANULES THAT ARE FORMED DURING BAND AND SEGMENTED NEUTROPHIL STAGE?

GRANULOCYTE-COLONY STIMULATING FACTOR (G-CSF)

MAJOR CYTOKINE RESPONSIBLE FOR THE STIMULATION OF NEUTROPHIL PRODUCTION?

GRANULOCYTE-MONOCYTE PROGENITOR (GMP)

COMMON PROGENITOR BETWEEN NEUTROPHILS AND MONOCYTES THAT IS DISTINCT FROM EOSINOPHILS AND BASOPHILS?

STEM CELL POOL

PROLIFERATION POOL

MATURATION POOL

3 POOLS OF DEVELOPING NEUTROPHILS?

7 HOURS

WHAT IS THE HALF LIFE OF NEUTROPHILS IN THE BLOOD?

INTEGRIN

SELECTIN

WHAT ALLOWS NEUTROPHILS TO MARGINATE AND EXIT THE BLOOD INTO THE TISSUES?

DIAPEDESIS

THE PROCESS BY WHICH THE NEUTROPHILS EXIT THE PERIPHERAL CIRCULATION INTO THE TISSUES?

Mcl-1 and MYELOPEROXIDASE

PRODUCTS OF INFLAMMATION AND INFECTION THAT TEND TO PROLONG THE NEUTROPHIL’S LIFE SPAN THROUGH ANTI-APOPTOTIC SIGNALS?

MAC-1

IT TRIGGERS THE DEATH AND PHAGOCYTOSIS OF NEUTROPHILS?

PROMYELOCYTE

WHAT STAGE OF NEUTROPHIL DEVELOPMENT?

THE NUCLEUS IS ROUND TO OVAL AND IS OFTEN ECCENTRIC. A PARANUCLEAR HALO ‘‘HOF’’ IS USUALLY SEEN IN THIS STAGE

PROMYELOCYTE

WHAT STAGE OF NEUTROPHIL DEVELOPMENT?

THE CYTOPLASM IS EVENLY BASOPHILIC AND FULL OF PRIMARY OR AZUROPHILIC GRANULES

MYELOCYTE

WHAT STAGE OF NEUTROPHIL DEVELOPMENT?

THE FINAL STAGE IN WHICH CELL DIVISION OR MITOSIS OCCUR

MYELOCYTE

WHAT STAGE OF NEUTROPHIL DEVELOPMENT?

THE STAGE WHEREIN PRODUCTION OF PRIMARY GRANULES CEASES AND CELLS BEGIN TO MANUFACTURE SECONDARY GRANULES

EARLY MYELOCYTE

WHAT STAGE OF NEUTROPHIL DEVELOPMENT?

THIS STAGE IS REFERRED TO AS ‘‘THE DAWN OF NEUTROPHILIA’’

MYELOCYTE

WHAT STAGE OF NEUTROPHIL DEVELOPMENT?

SECONDARY NEUTROPHILIC GRANULES SLOWLY SPREAD THROUGH THE CELL UNTIL ITS CYTOPLASM IS MORE LAVANDER-PINK THAN BLUE

METAMYELOCYTE

WHAT STAGE OF NEUTROPHIL DEVELOPMENT?

STAGE WHEREIN CELLS ARE NO LONGER CAPABLE OF DIVISION AND THE MAJOR MORPHOLOGIC CHANGE IS IN THE SHAPE OF THE NUCLEUS

METAMYELOCYTE

WHAT STAGE OF NEUTROPHIL DEVELOPMENT?

THE NUCLEUS IS INDENTED (KIDNEY BEAN SHAPED OR PEANUT SHAPED) AND CHROMATIN IS INCREASINGLY CLUMPED AND NUCLEOLI IS ABSENT

METAMYELOCYTE

WHAT STAGE OF NEUTROPHIL DEVELOPMENT?

THE SYNTHESIS F TERTIARY GRANULES MAY BEGIN DURING THIS STAGE

GELATINASE GRANULES

TERTIARY GRANULES ARE ALSO KNOWN AS?

METAMYELOCYTE

WHAT STAGE OF NEUTROPHIL DEVELOPMENT?

THE CYTOPLASM CONTAINS VERY LITTLE RESIDUAL RNA AND THEREFORE LITTLE OR NO BASOPHILIA

STAB OR BAND CELL

WHAT STAGE OF NEUTROPHIL DEVELOPMENT?

ALL EVIDENCE OF RNA (CYTOPLASMIC ASOPHILIA) ARE ABSENT AND TERTIARY GRANULES CONTINUE TO BE FORMED

BAND OR STAB CELL

WHAT STAGE OF NEUTROPHIL DEVELOPMENT?

SECRETORY GRRANULES MAY BEGIN TO FORM DURING THIS STAGE

SECRETORY VESICLES

SECRETORY GRANULES ARE ALSO KNOWN AS?

STAB OR BAND CELL

WHAT STAGE OF NEUTROPHIL DEVELOPMENT?

NUCLEUS IS HIGHLY CLUMPED. NUCLEAR INDENTATION EXCEEDS ONE HALF OF THE DIAMETER OF THE NUCLEUS, BUT NO ACTUAL SEGMENTATION YET.

TRANSCOBALAMIN 1 OR R-BINDER PROTEIN

ONE OF THE FUNCTION OF NEUTROPHILS IS TO SECRETE __ OR __, WHICH IS NECESSARY FOR THE PROPER ABSORPTION OF VITAMIN B12

1. PHAGOCYTOSIS

2. GENERATION OF NEUTROPHIL EXTRACELLULAR TRAP (NETs)

3. SECRETORY FUNCTION

WHAT ARE THE THREE FUNCTIONS OF NEUTROPHILS?

NEUTROPHIL EXTRACELLULAR TRAP

WHAT STRUCTURE HAVE ENZYMES FROM NEUTROPHIL GRANULES ATTACHED TO THEM AND HAVE BEEN SHOWN TO BE ABLE TO TRAP AND KILL G(+) AND G(-) BACTERIA AND FUNGI?

OXYGEN DEPENDENT

OXYGEN DEPENDENT OR OXYGEN INDEPENDENT?

RESPIRATORY BURST THROUGH THE ACTIVATON OF NADPH OXIDASE. H2O2 AND HYPOCHLORITE ARE PRODUCED

OXYGEN INDEPENDENT

OXYGEN DEPENDENT OR OXYGEN INDEPENDENT?

THE pH W/N THE PHAGOSOME BECOMES ALKALINE AND THEN NEUTRAL, THE pH AT WHICH DIGESTIVE ENZYME WORKS

HEMATOPOIETIC STEM CELL

WHAT CELLS CONSIST OF THE STEM CELL POOL?

COMMON MYELOID PROGENITOR CELLS, GRANULOCYTE-MONOCYTE PROGENITORS, MYELOBLASTS, PROMYELOCYTES, MYELOCYTES

WHAT CELLS ARE UNDER PROLIFERATION (MITOTIC) POOL?

COLONY-FORMING UNITS-GRANULOCYTES, ERYTHROCYTES,MONOCYTES, AND MACROPHAGE (CFU-GEMM)

COMMON MYELOID PROGENITOR CELL IS ALSO KNOWN AS?

METAMYELOCYTE

BAND OR STAB NEUTROPHILS

SEGMENTED NEUTROPHILS

WHAT CELLS ARE UNDER THE MATURATION (STORAGE) POOL?

MYELOCYTE

IDENTIFY THE CELL

PROMYELOCYTE

IDENTIFY THE CELL

NOTE: NO BLUSH OR PINKISH GRANULES, JUST DARK PURPLE

NOTE AT THE SIZE

EARLY MYELOCYTE

IDENTIFY THE CELL

NOTE AT:

1. THE SIZE

2. IS THERE PINK GRANULATION?OR ONLY BLUE AND PURPLE?

3. WHAT DOES THE CYTOPLASM LOOK LIKE?

METAMYELOCYTE

IDENTIFY THE CELL

NOTE:

WHAT DOES THE NUCLEUS LOOK LIKE?

STAB OR BAND NEUTROPHIL

IDENTIFY THE CELL

WHAT DOES THE NEUCLEUS LOOK LIKE?