Principles of Molecular Biology (Advanced) - DNA Replication II

A series of vocabulary flashcards based on the learning outcomes and concepts associated with DNA replication in E. coli and eukaryotic systems.

Helicase

Begins unwinding of DNA double helix.

DNA gyrase

Assists unwinding of DNA double helix.

Single Strand Binding (SSB) proteins

Stabilize single strands of DNA.

Primase

Synthesis of RNA primer.

DNA polymerase III

Elongation of DNA strand by DNA synthesis.

DNA polymerase I

Removal of RNA primer and filling in gap with DNA.

DNA ligase

Closes last phosphoester gap to form phosphodiester bond.

Replisome

The complex of proteins that work together to synthesize both leading and lagging DNA strands.

Primosome

The protein assembly in E. coli mediating primer synthesis including helicase and primase.

Termination of Replication

The process whereby replication forks meet at the terminus region requiring Tus protein.

Type I Topoisomerase

Enzyme that cuts one strand of DNA.

Type II Topoisomerase

Enzyme that cuts both strands of DNA.

Eukaryotic DNA polymerases

A group of enzymes in eukaryotes with different properties for replication and repair.