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What is biodiversity?
It includes:
Species diversity (different species)
Genetic diversity (differences within a species)
Ecosystem diversity (different habitats and ecosystems)
Why does biodiversity matter and how do we know?
Makes ecosystems more stable and resilient
Increases productivity (more plant growth)
Provides ecosystem services like food, clean water, pollination, and disease control
Helps ecosystems recover from disturbances (fires, droughts, pests) Experiments show ecosystems with more species perform better (higher productivity)
Long-term studies show diverse ecosystems recover faster after disturbances
Comparisons show biodiversity loss leads to ecosystem collapse, dead zones, or reduced yields
principles and components of the scientific process
logical way to answer questions using evidence.
Main components:
Observation
Question
Hypothesis (testable explanation)
Prediction
Experiment or data collection
Analysis
Conclusion
Communication
Key principles:
Based on evidence
Testable and repeatable
Open to revision
Conducted within a scientific community
Formulate a testable hypothesis and design a controlled experiment
changes one thing at a time.
Example:
One group gets fertilizer
One group does not
Everything else stays the same
Variables & groups
Independent variable: what you change
Dependent variable: what you measure
Control group: no treatment
Experimental group: gets the treatment
Interpreting data
Look for patterns
Compare groups
Decide if results support the hypothesis
Identify the independent and dependent variables and control/experimental groups
what you change, what you measure, the cause and the effect
What are control and experimental groups?
does not get the treatment, gets the treatment
different kinds of data
Quantitative data: numbers (height, mass, temperature)
Qualitative data: descriptions (color, behavior, presence/absence) 1.Compare control vs experimental
Look for patterns or differences
Decide if results support the hypothesis
Describe the different ways biological diversity can be measured and how estimates of diversity are calculated.
1. Species richness
Number of different species in an area
Example: 10 species vs. 3 species
2. Species evenness
How evenly individuals are spread among species
High evenness = similar numbers of each species
Low evenness = one species dominates
3. Diversity indices
Combine richness + evenness into one number
Common examples: Shannon index
Higher value = higher diversity
Describe methods of sampling used to estimate diversity.
Scientists estimate biodiversity by sampling using quadrats, transects, random or stratified sampling, and mark–recapture methods depending on the habitat and organisms.
Scientists sample an area (quadrats, transects)
Count species and individuals
Use equations to estimate total diversity
Rarefaction curves show how diversity changes with sampling effort
Practice calculating Shannon Index (H’)
Higher H’ = higher biodiversity
Accounts for richness + evenness
Explain why a rarefaction plot is made, what data are used, and how these plots are interpreted.
Formula
H′=−∑(pilnpi)H' = -\sum (p_i \ln p_i)H′=−∑(pi lnpi )
pi = proportion of individuals in species
ln\ln = natural log
Explain why a rarefaction plot is made, what data are used, and how these plots are interpreted.
To compare biodiversity fairly when sample sizes differ
What data are used?
Number of individuals sampled
Number of species observed
Axes
X-axis: number of individuals (or samples)
Y-axis: species richness
How to interpret
Steeper curve → many new species found quickly
Curve leveling off → most species have been sampled
Higher curve = higher species richness
Use biodiversity estimates to inform decisions
help scientists and managers:
Decide which areas to protect
Compare habitat quality
Detect environmental damage
Track restoration success
Example decisions
Protect areas with high H’
Increase sampling if rarefaction curve hasn’t leveled off
Restore habitats with low diversity
How does the tilt and shape of the Earth create both the seasons and atmospheric circulation patterns?
The Earth is round, so sunlight hits it unevenly
The equator gets more direct sunlight
The poles get less direct sunlight
The Earth is tilted as it orbits the Sun
Different parts of Earth receive more or less sunlight during the year
When the Northern Hemisphere is tilted toward the Sun → summer
When it is tilted away from the Sun → winter
Seasons are caused by tilt, not distance from the Sun
Warm air at the equator rises
Cool air near the poles sinks
Air moves from high pressure to low pressure
Earth’s rotation (Coriolis effect) causes air to curve
This creates global wind patterns (Hadley, Ferrel, Polar cells
How does energy move in an ecosystem?
Energy enters ecosystems as sunlight
Producers (plants, algae) capture sunlight by photosynthesis
Energy moves to consumers when organisms eat plants or other animals
Decomposers break down dead matter and use remaining energy
Energy flows in one direction
Energy is lost as heat at each step
Only a small fraction (~10%) moves to the next level
Use global, seasonal, or diurnal patterns of CO2 emissions to infer the relative magnitudes of gross primary production and respiration.
CO₂ taken out of the air by photosynthesis
CO₂ put into the air by plants, animals, and microbes
Daytime: CO₂ usually decreases → photosynthesis (GPP) > respiration
Night: CO₂ increases → only respiration is happening
Spring & summer (especially in Northern Hemisphere): CO₂ goes down → lots of plant growth, GPP > respiration
Fall & winter: CO₂ goes up → less photosynthesis, respiration > GPP
CO₂ is lowest after peak growing season and highest after winter → shows that plant photosynthesis strongly affects atmospheric CO₂, but respiration still adds CO₂ year-round
difference between climate and weather.
Short-term conditions
What’s happening today or this week
Example: “It’s raining and 45°F in North Carolina today”
Long-term average patterns (years to decades)
What you expect over time
Example: “North Carolina usually has mild winters and hot, humid summers”
Why does nutrient cycling matter for biodiversity?
All living things need nutrients (like nitrogen and phosphorus) to grow and survive.
keeps those nutrients moving through soil, water, plants, animals, and microbes.
When nutrients are available and recycled, more species can survive → higher biodiversity.
If nutrients are lost or limited, fewer species can live there → lower biodiversity.
Use a simple compartment model of nutrient cycling to account for all inputs and outputs for an ecosystem.
Soil
Plants
Animals
Decomposers (microbes)
Sunlight (energy, not recycled)
Nutrients from:
Weathering of rocks
Atmospheric deposition (rain, nitrogen fixation)
Soil → Plants (nutrient uptake)
Plants → Animals (eating)
Plants & Animals → Decomposers (death, waste)
Decomposers → Soil (mineralization)
Nutrient loss by:
Leaching into water
Runoff
Gas loss to the atmosphere (e.g., denitrification)
What are the components of the nitrogen cycle? Include the different forms of organic and inorganic nitrogen
N₂ (nitrogen gas) – in the atmosphere
NH₄⁺ (ammonium) – in soil and water
NO₂⁻ (nitrite) – in soil (short-lived)
NO₃⁻ (nitrate) – in soil and water
Amino acids
Proteins
DNA and RNA
Found in plants, animals, microbes, and detritus
the processes that convert nitrogen from one form to another.
1. Nitrogen fixation
N₂ → NH₄⁺
Done by bacteria (in soil, water, or plant roots) and lightning
2. Assimilation
NH₄⁺ or NO₃⁻ → organic nitrogen
Plants take up inorganic nitrogen and build it into tissues
3. Consumption
Nitrogen moves plants → animals through eating
4. Excretion & death
Organic nitrogen returns to soil as waste or dead material
5. Mineralization (decomposition)
Organic nitrogen → NH₄⁺
Done by decomposer microbes
6. Nitrification
NH₄⁺ → NO₂⁻ → NO₃⁻
Done by soil bacteria
7. Denitrification
NO₃⁻ → N₂ (gas)
Returns nitrogen to the atmosphere
Predict changes in pools or fluxes within a compartment model of nutrient cycling based on provided information.
where nutrients are stored
Soil nutrients
Plant biomass
Animal biomass
Detritus / decomposers
movement between pools
Uptake, consumption, decomposition, leaching, gas loss
If a flux increases, the receiving pool gets bigger, If a flux decreases, the receiving pool gets smaller
True or false
true
examples of flux and pool changes
More fertilizer added → soil nutrient pool increases → plant uptake increases
More plant growth → plant biomass pool increases → more nutrients for herbivores
Deforestation → plant pool decreases → nutrient loss from soil increases (leaching)
More decomposition → soil ammonium increases
Explain how the addition of nutrients affects the biodiversity of an ecosystem; and consider ways to prevent human-caused nutrient inputs. (short term)
More nutrients → faster plant growth
Productivity increases
Some species benefit
Explain how the addition of nutrients affects the biodiversity of an ecosystem; and consider ways to prevent human-caused nutrient inputs. (long term)
Fast-growing species outcompete others
Community becomes less diverse
Can cause algal blooms and dead zones in water
Preventing human-caused nutrient inputs
Fertilizers
Animal waste
Sewage and wastewater
Urban runoff
Use less fertilizer and apply it carefully
Plant buffer strips near rivers and lakes
Improve wastewater treatment
Use cover crops to absorb excess nutrients
Protect wetlands (they filter nutrients)