Master List of Molecular and Cellular Techniques to Detect Macromolecules

_________, ______________, ______________, _______________ are methods used to detect DNA

PCR, Southern blot, Genomic library, Dideoxy sequencing

Commonly asked questions for PCR are: is the _______ ___________ present, is the _______ _________ present, is ____________ present, is the __________ present in the bacteria?

DNA sequence, DNA mutation, polymorphism, plasmid

Commonly asked questions for Southern blot are: is the ________ _______ present, is the _____________ ________ present, how many _________ are present at a locus?

DNA sequence, chromosomal mutation, VNTRs

Commonly asked questions for Genomic library are: what is the __________ __________ of an organism, how can I easily obtain a _______ __________ from an organism for further molecular study?

genomic sequence, cloned sequence

Commonly asked questions for dideoxy sequencing are: is the intended _______ __________ present, what is the ______ __________ at a certain location?

DNA mutation, DNA sequence

__________ ___________ is a method used to detect DNA or mRNA through cDNA

Next-generation sequencing

Commonly asked questions for dideoxy sequencing are: what is the __________ __________ for an organism, what is the _______ of the organism/tissue/stage?

genomic sequence, exome

____________ and __________ are methods used to detect mRNA through cDNA

RT-PCR, Q-PCR

Commonly asked questions for RT-PCR are: what are the _____ _______ levels in an organism/tissue/stage?

gene expression

RT-PCR is __________ and _____________

indirect, qualitative

Commonly asked questions for Q-PCR are: what are the ________ ____________ levels in an organism/tissue/stage?

gene expression

Q-PCR is __________ and _____________

indirect, quantitative

_____________ _______ is a method used to detect RNA (usually mRNAs)

Northern blot

Commonly asked questions for Northern blots are: what are the _____ ____________ levels in an organism/tissue/stage?

gene expression

Northern blots are __________ and ___________ or ___________

indirect, qualitative, quantitative

________ ___________ and __________ ___________ are methods used to detect mRNA

cDNA library, reporter expression

Commonly asked questions for cDNA library are: what is the _____ of the organism/tissue/stage, how can I easily obtain a _______ ______ sequence from an organism for further molecular study?

exome, cloned DNA

Commonly asked questions for reporter expression are: _______ of promoter activity, and ________ measure of gene expression levels

detection, indirect

_________ _______ (or ____________) and _______________ are methods used to detect protein

western blot, immunoblot, immunofluorescence

Commonly asked questions for Western blot are: is a ________ present, how ______ is present, is an enzyme in its __________ form, is the protein __________ or otherwise altered?

protein, much, active, truncated

Commonly asked questions for immunofluroscence are: detection of ___________ and/or __________ ________ of a tagged protein, _______________ of two proteins

presence, subcellular localization, colocalization

__________ is a method used to detect DNA, RNA, or Protein

Microarray

Commonly asked questions for microarrays are: