Core prac 11 (hill reaction)

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Last updated 11:46 AM on 11/21/22
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9 Terms

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Independent variable
light intensity reaching samples of chloroplast
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Dependant variable
amount of DCPIP reduced (changes colour)
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Control variable to control pH
add 5ml buffer solution to each
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Control variable to control amount of colour change
add 10ml of DCPIP to each beaker so starting colour is similar
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Control variable to control how much photosynthesis could occur
allow to sit for the same amount of time in the dark or infront of the lamp
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Control variable to control rate of max photosynthesis
use same species of plant
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Why use DCPIP
DCPIP changes colour when electrons are released
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Why are electrons released
the light dependant reaction releases electrons when excited by light that is normal used to reduce NADP or ATP
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How to separate the chloroplast after crushing spinach
use a centrifuge and the green pellets at the bottom will be the chloroplast