Erwin chargaff

Who is Erwin Chargaff?

A biochemist known for discovering Chargaff's Rules regarding base pairing in DNA.

What are Chargaff's Rules?

In DNA, the amount of adenine (A) equals thymine (T), and the amount of cytosine (C) equals guanine (G), establishing a 1:1 ratio.

What is the significance of Chargaff's discovery?

It established that the ratio of base pairs is consistent across species, leading to key insights in DNA structure.

What technique did Chargaff use to separate DNA bases?

Paper chromatography.

How did Chargaff measure the concentration of DNA bases?

Using UV spectrophotometry after separating the bases with paper chromatography.

When did Chargaff make his discovery?

During the 1950s at Columbia University in New York.

What is the relationship between purines and pyrimidines according to Chargaff's findings?

The total amount of purines equals the total amount of pyrimidines in DNA.

What was the birthplace of Erwin Chargaff?

Chernivtsi, Ukraine.

What was Erwin Chargaff's date of birth?

August 11, 1905.

What method measures UV and visible light absorption in samples?

UV spectrophotometry.