DNA Replication Enzymes

DNA Polymerase I

Lagging Strand Repair (Okazaki Strand Processing). Removes RNA primers and replaces them with DNA nucleotides

DNA Polymerase III

Synthesizes new DNA using template strand (continuous on leading and not-continuous on lagging). Adds nucleotides to growing end (3’)

Helicase

Breaks Hydrogen bonds between base pairs, allowing for strand separation

Primase

Synthesizes RNA primers neede to start DNA synthesis. Provides the 3’-OH group for DNA polymerase to extend for

Ligase

Seals nicks between fragments to create continuous DNA strand, Joins Okazaki fragments by forming phosphodiester bonds

Topoisomerase

Prevents coiling during DNA unwinding

Single Strand Binding Proteins (SSBPs)

Bind to separated DNA strands to prevent reconnecting. Stabilizes single-stranded DNA during replication