Drug Testing

*Question: What is the minimum floor area requirement for a screening drug test laboratory?
A) 10 sq.m. total floor area
B) 20 sq.m. floor area with 10 sq.m. working area
C) 30 sq.m. floor area with 15 sq.m. working area
D) 60 sq.m. floor area with 30 sq.m. working area

*Answer: B) 20 sq.m. floor area with 10 sq.m. working area

*Question: What is the primary analytical method used in confirmatory drug testing laboratories?
A) ELISA immunoassay
B) Thin layer chromatography (TLC)
C) Gas chromatography-mass spectrometry (GC-MS)
D) Fluorescence polarization immunoassay

*Answer: C) Gas chromatography-mass spectrometry (GC-MS)

*Question: What is the acceptable temperature range for freshly voided urine in drug testing?
A) 25-30°C
B) 32-38°C
C) 36-40°C
D) 30-35°C

*Answer: B) 32-38°C

*Question: What is the acceptable pH range for valid urine specimens in drug testing?
A) 3.0-8.0
B) 4.0-9.0
C) 5.0-8.0
D) 4.5-8.5

*Answer: B) 4.0-9.0

*Question: What specific gravity reading would indicate that a urine sample has been substituted with water?
A) 1.003
B) 1.010
C) 1.000
D) 1.005

*Answer: C) 1.000

*Question: What is the acceptable specific gravity range for valid urine specimens?
A) 1.000-1.025
B) 1.005-1.035
C) 1.003-1.030
D) 1.010-1.030

*Answer: C) 1.003-1.030

*Question: What is the required urine volume for drug testing?
A) 15-30 mL
B) 30-60 mL
C) 60-100 mL
D) 20-40 mL

*Answer: B) 30-60 mL

*Question: Which type of specimen tampering involves adding bleach, ammonia, or hydrogen peroxide to urine?
A) Dilution
B) Substitution
C) Adulteration
D) Contamination

*Answer: C) Adulteration

*Question: How can external dilution of a urine specimen be detected?
A) Checking the temperature
B) Checking the specific gravity
C) Checking the color
D) Checking the pH

*Answer: B) Checking the specific gravity

*Question: What is the minimum retention period for negative drug test specimens?
A) 3 days after receipt of result
B) 5 days after receipt of result
C) 7 days after receipt of result
D) 10 days after receipt of result

*Answer: B) 5 days after receipt of result

*Question: What is the minimum retention period for positive drug test specimens?
A) 5 days upon receipt of result
B) 10 days upon receipt of result
C) 15 days upon receipt of result
D) 30 days upon receipt of result

*Answer: C) 15 days upon receipt of result

*Question: Who can serve as the head of a screening drug test laboratory?
A) Any licensed medical technologist
B) A doctor, often a pathologist or physician
C) A registered chemist only
D) A pharmacist with training

*Answer: B) A doctor, often a pathologist or physician

*Question: Who must be employed full-time in a confirmatory drug testing laboratory?
A) A medical technologist with GCMS training
B) A pharmacist with chromatography training
C) A chemist with training in chromatography and mass spectroscopy
D) A chemical engineer with screening training

*Answer: C) A chemist with training in chromatography and mass spectroscopy

*Question: What document is used to track the handling of specimens throughout the drug testing process?
A) Laboratory Request Form
B) Custody and Control Form
C) Specimen Collection Log
D) Drug Test Authorization Form

*Answer: B) Custody and Control Form

*Question: Which specimen type is most commonly used for drug testing?
A) Blood
B) Saliva
C) Urine
D) Hair

*Answer: C) Urine

*Question: What is the required blood volume for drug testing?
A) Minimum 3 mL in a 5 mL tube
B) Minimum 5 mL in a 10 mL tube
C) Minimum 7 mL in a 10 mL tube
D) Minimum 10 mL in a 15 mL tube

*Answer: B) Minimum 5 mL in a 10 mL tube

*Question: What is the advantage of using hair as a specimen for drug testing?
A) It is the least expensive option
B) It provides the fastest results
C) It detects chronic substance use
D) It has the highest accuracy

*Answer: C) It detects chronic substance use

*Question: How much hair is required for drug testing?
A) 50 mg
B) 75 mg
C) 100 mg
D) 150 mg

*Answer: C) 100 mg

*Question: What type of immunoassay is used in screening drug tests?
A) Sandwich assay
B) Competitive assay
C) Direct assay
D) Indirect assay

*Answer: B) Competitive assay

*Question: In a drug screening immunoassay, what does the absence of a colored line indicate?
A) Negative result
B) Invalid test
C) Positive result
D) Inconclusive result

*Answer: C) Positive result

*Question: Why does "no line" indicate a positive result in drug screening tests?
A) The antibodies degraded during testing
B) Drug antigens in urine bound antibodies early, leaving none for the immobilized drug
C) The immobilized drug was consumed by the sample
D) The control line failed to develop

*Answer: B) Drug antigens in urine bound antibodies early, leaving none for the immobilized drug

*Question: What is the turnaround time (TAT) for a screening drug test?
A) 15 minutes
B) 30 minutes
C) 1 hour
D) 24 hours

*Answer: B) 30 minutes

*Question: What is the turnaround time (TAT) for a confirmatory drug test?
A) 3 days
B) 7 days
C) 15 days
D) 30 days

*Answer: C) 15 days

*Question: How long are drug testing results valid?
A) 6 months
B) 1 year
C) 2 years
D) 3 years

*Answer: B) 1 year

*Question: What is a re-test in drug testing?
A) Testing a new sample from the same patient
B) A replicate test to check the initial test result of the same sample
C) Testing performed when results are legally questioned
D) Annual quality control testing

*Answer: B) A replicate test to check the initial test result of the same sample

*Question: What is a challenge test?
A) A routine quality control procedure
B) A replicate test when confirmatory results are legally questioned
C) The initial screening test
D) A test performed on a different specimen type

*Answer: B) A replicate test when confirmatory results are legally questioned

*Question: Who pays for a challenge test?
A) The laboratory
B) The government health agency
C) The complainant
D) Insurance company

*Answer: C) The complainant

*Question: When must specimens be collected inside the drug testing facility?
A) Always, with no exceptions
B) Ideally, except for allowed remote collections
C) Only for confirmatory testing
D) Only for pre-employment testing

*Answer: B) Ideally, except for allowed remote collections

*Question: Which of the following is an acceptable location for remote specimen collection?
A) Patient's home for convenience
B) Random school drug tests
C) Private clinic without authorization
D) Collection center without observation

*Answer: B) Random school drug tests

*Question: What must be performed when specimen collection occurs outside the drug testing facility?
A) Immediate confirmatory testing
B) Sample validity tests
C) Duplicate specimen collection
D) Witnessed collection by two observers

*Answer: B) Sample validity tests

*Question: What is the purpose of validity testing?
A) To determine drug concentration
B) To ensure the specimen is freshly collected and not adulterated
C) To identify the specific drug present
D) To calculate the turnaround time

*Answer: B) To ensure the specimen is freshly collected and not adulterated

*Question: When is validity testing performed?
A) After the drug test is completed
B) Before the actual drug test
C) Only if results are positive
D) Only for confirmatory testing

*Answer: B) Before the actual drug test

*Question: What should the appearance of a valid urine specimen be?
A) Slightly cloudy
B) Clear
C) Milky
D) Turbid

*Answer: B) Clear

*Question: What type of odor should a valid urine specimen have?
A) Ammoniacal
B) Aromatic
C) Odorless
D) Sweet

*Answer: B) Aromatic

*Question: At what temperature should urine specimens be stored for prolonged periods?
A) 4°C
B) -10°C
C) -20°C
D) -80°C

*Answer: C) -20°C

*Question: What container is used for collecting urine specimens for drug testing?
A) Glass container
B) Metal container
C) Polyethylene specimen container
D) Sterile culture tube

*Answer: C) Polyethylene specimen container

*Question: How much saliva is required for drug testing?
A) 1 mL
B) 2 mL
C) 3 mL
D) 5 mL

*Answer: B) 2 mL

*Question: What is the wearing period for an FDA-approved sweat patch used in drug testing?
A) 1-3 days
B) 3-5 days
C) 1-2 weeks
D) 2-4 weeks

*Answer: C) 1-2 weeks

*Question: Why is blood not commonly used for drug testing despite being the most accurate?
A) It degrades too quickly
B) It is expensive and impractical
C) It cannot detect most drugs
D) Collection is too dangerous

*Answer: B) It is expensive and impractical

*Question: What measures ensure the integrity of urine specimens during collection?
A) Using preservatives in the container
B) Coloring agents in toilet bowl and removal of outer garments
C) Collecting specimens at home
D) Allowing unsupervised collection

*Answer: B) Coloring agents in toilet bowl and removal of outer garments

*Question: What information is included on specimen labels?
A) Patient name and address
B) Date/time of collection, signature of client and ASC, specimen ID number
C) Doctor's name and license number
D) Drug test result and interpretation

*Answer: B) Date/time of collection, signature of client and ASC, specimen ID number

*Question: Why is the patient's name not included on specimen labels?
A) For privacy reasons only
B) To prevent testing bias
C) To save space on the label
D) Due to regulatory requirements

*Answer: B) To prevent testing bias

*Question: What does GC measure in GC-MS analysis?
A) Mass-to-charge ratio
B) Retention time
C) Molecular weight
D) Drug concentration

*Answer: B) Retention time

*Question: What does MS measure in GC-MS analysis?
A) Retention time
B) Drug solubility
C) Mass-to-charge ratio
D) pH levels

*Answer: C) Mass-to-charge ratio

*Question: Why is GC-MS preferred over GC alone for confirmatory testing?
A) It is less expensive
B) It provides faster results
C) The combination of retention time and mass-to-charge ratio gives high specificity
D) It requires less sample volume

*Answer: C) The combination of retention time and mass-to-charge ratio gives high specificity

*Question: What does a screening test determine?
A) Exact concentration of the drug
B) Presence or absence of a drug
C) Chemical structure of the drug
D) Metabolism pathway of the drug

*Answer: B) Presence or absence of a drug

*Question: What additional information does a confirmatory test provide beyond screening?
A) Only the presence or absence of drug
B) Identity of the metabolite and concentration of the drug
C) Only the drug concentration
D) Only the drug identity

*Answer: B) Identity of the metabolite and concentration of the drug

*Question: What does "presumptive positive" mean in screening test results?
A) Below the cutoff level
B) At or above the cutoff level
C) Requires immediate confirmatory testing
D) Invalid result

*Answer: B) At or above the cutoff level

*Question: What does "presumptive negative" mean in screening test results?
A) Drug is definitely absent
B) Below cutoff level; drug may be absent or present in low amounts
C) Above the cutoff level
D) Sample is contaminated

*Answer: B) Below cutoff level; drug may be absent or present in low amounts

*Question: What type of test tube is used for blood specimen collection in drug testing?
A) EDTA tube
B) Heparinized tube
C) Plain test tube
D) Sodium citrate tube

*Answer: C) Plain test tube

*Question: Which laboratory serves as the National Reference Laboratory for drug confirmatory testing?
A) Philippine General Hospital
B) East Avenue Medical Center (EAMC)
C) Research Institute for Tropical Medicine
D) National Bureau of Investigation

*Answer: B) East Avenue Medical Center (EAMC