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These flashcards cover the key concepts, methods, and details regarding glucose testing in urine and related procedures.
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What does a high concentration of glucose in urine indicate?
It may indicate that the person has diabetes.
What normal range of glucose concentration should be present in urine?
The normal range is 0-0.8mM.
What reagent is used to test for glucose in urine?
Benedict's reagent.
What are the two types of Benedict's reagent?
Qualitative and Quantitative.
What does Quantitative Benedict's reagent contain?
It contains copper sulfate.
How is the amount of reducing sugar measured in a Quantitative Benedict's test?
By the disappearance of the blue color of copper sulfate.
What is created from measuring known concentrations of glucose using a colorimeter?
A calibration curve.
What is the effect of higher glucose concentration on solution color?
The solution becomes paler as glucose concentration increases.
What is the dilution factor when creating a 1M solution from a 2M solution?
1:2 dilution.
What is the purpose of adding a test tube of pure water in the dilution process?
To serve as a control.