Activity 1: Wet Mount and Hanging Drop

Wet Mount

It is a method where the specimen is suspended in a drop of liquid between slide and a cover slip.

Hanging Drop

It is a method where the specimen is suspended in a drop of liquid with the use of a depression slide.

Petroleum Well

In the hanging drop method, what was utilized because there are no depression slides available?

Glucose

What do Yeasts love to eat in order to be active?

Carbon Dioxide

What does yeast produce after eating sugar that causes dough to rise?

Saccharomyces cerevisiae

What is the name of the fungi for the Live Baker’s Yeast?

Negative

What gram of bacteria is more motile?

thin peptidoglycan and complex flagellar structures

Why most gram negative are motile?

monotrichous

bacteria with only one flagella.

lophotrichous

bacteria with numerous flagella stemming from a single origin.

peritrichous

Bacteria with numerous flagella but it is scattered in the whole body.

amphitrichous

bacteria with only two opposite flagella in each side.

Long

Motile organisms are seen moving at ___ distances.

Brownian Movement

caused by water molecules bouncing around the solution knocking up against each other even non-motile are knocked up causing fake movement (vibrations)/

due to cover slip pressing down the liquid

Why is wet mount poor regarding true motility?

Dries

In the wet mount, the surface area of the pressed liquid is much larger than that of the hanging drop. What would happen to the liquid of the wet mount if been stay put for a long time?

Wet Mount

Where does liquid dry faster? Wet mount or hanging drop?

Traps microorganisms to the side of the coverslip .

What is the job of surface tension in Wet mount?

Surface Tension

Microorganisms may be trapped at the edge of the cover slip affecting organismal movement. What is being referred to?

Water

Bacteria has refractive index closely approxiamte to ___.

Decomposing hay and air bubbles

What are the possible debris that would be seen in a hay infusion under the microscope?

Amoebas

They may be find under hay infusion, they are irregular shaped and slow moving as they use pseudopods.

round/ oval shaped

Shape of a live bakers yeast under the microscope

Transparent but internal granules are visible

Describe the color of the live bakers yeast.

lacks motility

Describe motility of Live Baker’s yeast

Brownian Movement

What makes live baker’s yeast move?

Pseudohyphae

What do you call the short chains that lve baker’s yeast do where the budding of the yeast is attached to the mother yeast before separating.

Lichen spore

Sac like structures under the microscope and in clusters.

Sac like

What is the shape of a lichen spore?

Hyphae

Thin branching filaments alongside lichen spores.