BC Lab Protein Colors (Analyte + posi)
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20 Terms
What is the positive result of the Biuret test?
Correct answer: Pink to violet to blue coloration
What is the analyte detected by the Biuret test?
Correct answer: Peptide bonds in proteins
What is the positive result of the Ninhydrin test?
Correct answer: Blue to blue-purple color (yellow-orange for proline)
What is the analyte detected by the Ninhydrin test?
Correct answer: Free α-amino acids and amines
What is the positive result of the Xanthoproteic test?
Correct answer: Yellow solution on heating, turns orange with excess NaOH
What is the analyte detected by the Xanthoproteic test?
Correct answer: Aromatic amino acids (Tyrosine, Tryptophan, Phenylalanine)
What is the positive result of Millon's test?
Correct answer: Old rose or flesh purple-red precipitate
What is the analyte detected by Millon's test?
Correct answer: Phenolic groups in tyrosine
What is the positive result of the Hopkins-Cole test?
Correct answer: Pink to violet interface color (Cole rhymes with Indole)
What is the analyte detected by the Hopkins-Cole test?
Correct answer: Indole groups in tryptophan
What is the positive result of the Sakaguchi test?
Correct answer: Red to red-orange coloration
What is the analyte detected by the Sakaguchi test?
Correct answer: Guanido groups in arginine
What is the positive result of Fohl's (Lead Acetate) test?
Correct answer: Brown or black precipitate (PbS)
What is the analyte detected by Fohl's test?
Correct answer: Sulfur-containing amino acids (methionine, cysteine)
What is the positive result of Pauly's test?
Correct answer: Red azo dye color
What is the analyte detected by Pauly's test?
Correct answer: Histidine and tyrosine (We are His Tyr ical when sir Paul is around)
What is the positive result of the Nitroprusside test?
Correct answer: Red coloration (like the stuff that comes out of it every month)
What is the analyte detected by the Nitroprusside test?
Correct answer: Sulfhydryl groups (-SH), such as in cysteine (as in SHut up if you c it IYKWIM)
What is the positive result of the Amide test?
Correct answer: Red blue litmus paper indication
What is the analyte detected by the Amide test?
Correct answer: 1°, 2°, 3° amides and nitriles
Note 
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