Classes of Restriction Enzymes
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Last updated 7:53 PM on 10/29/25
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9 Terms
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Type I
cleave at sites remote from recognition site
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Type II
cleave w/in or at a short specific distance from the recognition site
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which class of restriction enzyme is most commonly used
type II
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Type III
cleave at sites a distance of 20-30 bp from recognition site
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Type IV
recognize methylated/glycoslyated DNA
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Type V
recognize non-palindromic sequences using guide RNAs
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purpose of DNA methylation
to protect native DNA from being cut by restriction enzymes
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Where are methyl groups placed on DNA?
at the recognition site on the DNA
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Average amount of times Type II cuts per base
every 4^n bases where n is the number of bases in the recognition sequence
Note 
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