Micro Lab Quiz #3

Ch. 7 and 20

sepsis

bacterial contamination

asepsis

absence of significant contamination

aseptic technique

prevent microbial contamination

sterilization

remove and destroy all microbial life

commercial steriliation

killing endospore (C. botulinum) from canned goods

disinfection

destroying harmful microorganism

antisepsis

destroying harmful microorganisms from living tissue

degerming

mechanical removal of microbes from limited area

sanitization

lowering/reducing microbial count to levels considered safe on objects and surfaces

biocide/ germicide

treatments that kill microbes

effectiveness of treatment depends on:

• number of microbes

• environment (pH/organic matter, temp, biofilm)

• time of exposure

• microbial characteristics

• concenctration of disinfectant

3 ways microbial control agents do:

1. alter membrane permeability

2. protein damage (enzymes)

3. nucleic acid damage

3 types of moist heat sterilization:

1. boiling

2. free-flowing steam

3. autoclave

what does moist heat sterilization do to kill microbe?

denatures proteins

what is settings of the autoclave?

121C/ 15 psi/ 15 min

what do autoclaves do?

kill all organisms/ endospore by using pressure to raise temp above boiling point

Pasteurization

Process of heating at certain temp to prevent food spoilabe

3 treatment of heat/pasteurization :