Differential Staining Acid-Fast

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6 Terms

1

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Acid Fast Stains

Differentiate cells based on the
presence or absence of mycolic
acid

2

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Mycolic acid (lipid)

waxy substance that has a high affinity for the primary stain
and resists decolorization by acid alcohol

3

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Genera separated by

Mycobacterium and Nocardia

4

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Acid Fast Stains – Kinyoun Method

Create a heat fixed smear
• Cover the smear with Kinyoun carbolfuchsin for 5-10
minutes
• Rinse with distilled water
• Decolorize with acid-alcohol until the run-off is clear
• Rinse with distilled water
• Apply methylene blue counterstain for 1 minute
• Rinse with distilled water.
• Blot on Bibulous paper
• Observe under oil immersion