dna analysis

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22 Terms

1
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What does DNA stand for?

Deoxyribonucleic Acid

2
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What is the function of DNA?

DNA stores information and contains instructions for making proteins.

3
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Where is nuclear DNA located?

In the nucleus of the cell.

4
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How many pairs of chromosomes do humans have?

Humans have 23 pairs of chromosomes.

5
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What are the two types of sex chromosomes?

XY for males and XX for females.

6
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What makes up the majority of human DNA?

Non-coding genes make up 90 to 95% of DNA.

7
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What are the four nucleobases of DNA?

Adenine, Guanine, Thymine, and Cytosine.

8
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What pairs with Thymine in DNA?

Adenine.

9
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What sugar does RNA contain?

Ribose sugar.

10
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What is the main process that produces RNA from DNA?

Transcription.

11
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What is the difference between transcription and replication?

Transcription produces a single strand of RNA, while replication copies the entire DNA molecule.

12
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What is the role of tRNA in translation?

tRNA translates the language of nucleotide bases into amino acids.

13
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What is the Central Dogma of molecular biology?

DNA is transcribed to RNA, which is then translated into proteins.

14
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What are RFLPs in DNA profiling?

Restriction Fragment Length Polymorphisms are variations in DNA fragments due to cuts by restriction enzymes.

15
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What is polymerase chain reaction (PCR)?

A technique used to amplify DNA, creating many copies of a specific DNA sequence.

16
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What is a Short Tandem Repeat (STR)?

A location on DNA where a short sequence of bases is repeated.

17
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How is DNA visualized during electrophoresis?

By applying a current that causes negatively charged DNA to move through a gel.

18
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What does CODIS stand for?

Combined DNA Index System.

19
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What is the purpose of CODIS?

To maintain a national database of DNA profiles for investigating crimes.

20
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What is the function of the ribosome in translation?

Ribosomes facilitate the attachment of tRNA to mRNA to synthesize proteins.

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What are the three tiers of CODIS?

Local, State, and National DNA indexes.

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Why is non-coding DNA important for DNA profiling?

The sequences in non-coding regions vary greatly from person to person, making them useful for individual identification.