To Investigate The Effect of Heat Denaturation on Catalase Activity

Apparatus

Beaker, water, hot plate, thermometer, test tube, graduated cylinder, pH9 buffer solution, celery, hydrogen peroxide

Step 1

Place 5g of chopped celery into a test tube and place it into a water bath at 100 degrees Celsius for 10 minutes

Step 2

Remove the test tube and let it cool

Step 3

Add 20cm cubed of pH9 buffer solution into the graduated cylinder

Step 4

Add a drop of washing up liquid

Step 5

Add 5g of the heated celery to the cylinder

Step 6

Add 2cm cubed of hydrogen peroxide to a test tube

Step 7

Stand the graduated cylinder and test tube in a water bath until the desired temperature (25 degrees Celsius)

Step 8

Pour hydrogen peroxide into the cylinder

Step 9

Note absence of foam and record

Step 10

Repeat procedure from step 5 using unheated celery to act as a control

Draw a diagram of the experiment

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