Microbiology 2460 Lab Final

What is the density if water at room temperature

1 g/mL

UTA microbiology lab classrooms are considered

BSL 2

GLP means

Good Laboratory Practice

Aseptic techniques are a combination or protocols to maintain

Sterility

Which of the following is the proper way to flame an inoculating loop?

Hold it at a 45 angle and allow the entire wire to turns red hot

Lack of proper aseptic technique during clinical procedure can lead to

Sepsis and high fever

Accuracy refers to the ability to transfer a volume of liquid that is close to the true or nominal value as indicated by the tool itself (e.g. pipettor)

True

The act of handwashing is commonly referred to as

Degerming

Where would you dispose of contaminated or used petri dishes

Red biohazard bin

Agar plates are stored upside down to prevent the following

Contamination and water condensation

is a method that involves the use of different chemicals to determine how susceptible a bacterium is to different antiseptics and disinfectants

Disk diffusion assay

To determine the effectiveness of a certain chemical agent, ____ is measured in ____

Zone of inhibition, millimeter

A test done to determine the susceptibility or resistance of specific microbes to various antimicrobial drugs is known as

Kirby-Bauer disk diffusion

Choose the best option that fits how Q13's answer is performed in a stepwise manner

Label plates, divide into quadrants, pipette bacteria culture, place antibiotics on plate

Antibacterial dilution tests are used to determine a particular's drug MIC, which stands for

Minimal Inhibitory Concentration

MIC is the ____ concentration of drug that ___ visible bacterial growth

Lowest, inhibits

A type of media that allows the growth of only specific microorganisms while inhibiting the growth of others is called

Selective media

Which number is pointing to the Coarse adjustment knob and revolving nose piece

4, 2

Which lens require the use of immersion oil

100x objective lens

You are viewing a specimen with a microscope that has an ocular lens with 10x magnification and an objective lens with 100x magnification. What is the total magnification

1000x

Which may lead to a false negative in a Gram stain

Cells are too old and have damaged cell walls

What is the purpose of immersion oil in microscopy

To increase numerical aperture

___ and ___ affect resolution

Wavelength and numerical aperture

To view your bacterial cell, the slide is placed on a platform known as

Stage

Gram positive bacteria appear under the microscope as

purple

Which is used to examine and observe biofilm development

Crystal violet

Which of the following is a common characteristic if biofilm

Formation of a protective extracellular matrix

Glycolysis is considered an anabolic reaction

False

In the API test, the URE test detects a microbe's ability to break down urea. This is considered a ____ reaction

Catabolic

What is the total magnification when viewing a microscope with a 100x objective lens and a 10x ocular lens

1000x

Streptococcus refers to:

Chains of cocci cells

What color is Gram-negative organism?

Pink/Red

Cellular morphology refers to the pattern that forms during cellular division

False

In a Gram stain, iodine is considered a ____ and makes dye less soluble so it adheres to the cell wall

Mordant

Which best describes anabolism

When small molecules are assembled into larger ones

An indirect ELISA can detect for current COVID infections only

False

The spike protein of the SARS-COV-2 virus is considered a/an

Antigen

If you have a patient that tests positive in the ELISA performed during Lab #5, then the patient:

had a past infection, has an active infection, and has successfully been vaccinated

If you are vaccinated for a disease like the flue virus, this means your body can produce ___ that work against viral ___

antibodies; antigens

One explanation for higher rates of antibiotic resistance in biofilms is that there is frequent exchange of extrachromosomal DNA among bacteria that reside within the biofilm

True

One explanation for higher rates of antibiotic resistance within biofilms is that bacterial cells found deep within the biofilm have higher metabolic activity

False

Staphylococcus aureus and Clostridium difficile are both common biofilm formers and are associated with hospital acquired infections (HAI)

True

ELISA uses two types of antibodies which are ___ and ___

primary and secondary

Full meaning of ELISA is

Enzyme Linked Immunosorbent Assay