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One step
-pentobarbitol in conscious animal
-gunshot
Two step
Captive bolt with an adjunct: IV salts, exsanguination, pithing, second shot
Other acceptable methods
• Electrocutions (220V), not used very often
• CO2 (piglets and kids only)
NOT acceptable methods
• Manual blunt trauma (exception swine neonates)
• IV disinfectants
• Conscious/sedated adjunct
• 120V electrocution
• Drowning
• Air embolism
Cow captive bolt landmarks

Goat captive bolt landmarks

Llama capitive bolt landmarks

Pig captive bolt landmarks

Effective stun
• Drop and stays down
• No corneal reflex, pupils central
• No normal rhythmic breathing
Confirming death
o Heartbeat and respiratory effort
o May take a few minutes
o Rigor is definitive