Laser Scanning Confocal Microscopy

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4 Terms

1
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Why does laser scanning confocal microscopy exist? (2)

- Because different levels of focus REDUCES crisp image
- Above + Below plane of focus interfere with light rays

2
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How does Laser Scanning Confocal Microscopy correct this? (3)

- Focus laser beam that scans at a single depth
- Emitted light is focused through a pinhole
- Fluorescence also used

3
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Why is there a pinhole?

So other light rays from other focal planes don't enter

4
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What is an advantage of doing it many times?

Computer can generate 3D image