Lab Animal Final Exam Review

Which of the following species is the most commonly used in laboratory animal science?

Rat and mouse

Which of the following is required to obtain certification in laboratory animal science?

No degree in Veterinary Medical Technology is required, but it does help!

Which of the following indicates that you are certified as a Laboratory Animal Technologist?

LATG

What does AALAS stand for?

American Association for Laboratory Animal Science

AALAS was established in 1967 for what purpose?

To establish the minimum standard of care for animals used in research.

Who is responsible for designing the experiment, analyzing the data, and reporting the results of a study?

Primary Investigator

Who is responsible for coordinating all animal care and compliance with regulations in research?

Laboratory Animal Veterinarian

Which of the following protect pet owners from pet theft for resale or exhibition?

AWA (Animal Welfare Act)

Which of the following species are covered by the PHS Policy but NOT by the AWA?

Rats and mice bred exclusively for research

Which of the following covers all species used in research and requires adherence to ethics and all regulations?

US Gov Principles

Which of the following agencies enforces adherence to the AWA and the Ag Guide?

USDA-APHIS

Which of the following agencies enforces adherence to PHS Policy and the Guide?

NIH-OLAW

What does IACUC stand for?

Institutional Animal Care and Use Committee

Which of the following is responsible for approval of all Animal Use protocols for animals used in research?

IACUC

No more than how many members of the IACUC may be affiliated directly with the institution conducting the research?

3

What are the Three R's of Ethics in animal research?

Replace, Reduce, Recycle

What is a disaster plan?

Response to a natural disaster impacting the research facility

What is a biosecurity plan?

Protects animals, agents, and materials from vandalism and theft

What is the purpose of the OSHP?

Protects your health and safety while working at a research facility.

What is a hazard?

A material or system with the potential to cause harm.

What is a risk?

The likelihood that danger will occur and impact your health and safety.

Which of the following are you NOT encouraged to report to OSHP?

 

A recent dental cleaning

Which of the following would NOT be disposed of in a 'sharps' container?

Soiled exam gloves

Broken glass should be cleaned up properly using which of the following methods?

Dustpan and broom

Sharps containers should be sealed for disposal once they are how full?

 

2/3 full

Which location would be most appropriate for disposal of contaminated latex gloves?

Biohazard bag or barrel

True or False: Loud noises in a work area are considered a physical agent/hazard.

True

True or False: A carcinogen is a chemical agent/hazard.

True

You are bitten by an animal at the facility.  The first thing you should do is:

Scrub the site with soap and warm water if bleeding and tell your boss!

Which animal used in research is most frequently responsible for allergies in personnel?

Rats

If you develop an allergy while working with any species, you should:

Temporarily stop working with that species until stabilized with treatment.

Which of the following is NOT an essential characteristic of animal caging?

Fashionable

Which of the following materials would NOT be appropriate for producing animal caging?

Wood

Which of the following is of no concern when cracks and crevices in animal caging are present?

Animals may be able to detect odors

Why is identical caging important for housing animals on the same research study?

Prevents the introduction of unknown variables

Which of the following types of caging is most commonly used for rats and mice in research?

Shoebox rodent cage

Which of the following cages would you use for measurement of urine and feces in a study?

Metabolism cage

Which cage type would be most appropriate for housing multiple cats, sheep, or non-human primates in the same cage?

Gang cage

What type of cage is permitted to be made of wood or cardboard?

Transport container for shipping

What is this device used for in laboratory animal science?

Restraint of rats and mice

What does the term 'lixit' refer to in laboratory animal science?

The spout which provides water with an automatic watering system.

What is the most likely cause for this condition shown in the image below?

Low humidity

If odors are present in an animal room, which of the following needs to be evaluated?

Ventilation

For which of the following would negative pressure be most appropriate?

Isolation & Quarantine

Why should animals with a specific microbiological status be housed in positive pressure rooms?

Positive pressure prevents outside contaminants from coming into the room.

A light/dark cycle written as 14/10 or 14:10 tells you that the animals should receive how many hours of darkness?

10

Bioexclusion rooms should be maintained under which type of pressure?

Positive

Biocontainment rooms should be maintained under which type of pressure?

Negative

Which of the following is considered a barrier?

Biosafety cabinets

PPE
Ventilation

The suffix '-static' stands for:

Inhibits growth

The suffix '-cidal' stands for:

Kill

Sterilization performs which of the following?

Kills all microorganisms that are present on a surface.

You would use a RODAC plate to perform which of the following in the animal room?

Testing of cleaning, disinfection, and sterilization procedures

True or False: The microenvironment is the area surrounding the animal's cage such as the animal room and even the facility beyond.

False

True or False: The microenvironment is the area within the animal's cage, run, or pen where the animal is housed.

True

True or False:Bedding and feed should be stored in the animal room to ensure easy access to these items.

False

Which of the following is considered the most important nutrient?

Water

Which of the following is a building block for proteins?

Amino Acids

An essential amino acid is considered essential because:

It can only be obtained in the diet.

Which of the following serves as the primary and the most rapid source of energy in the body?

Carbohydrates

What is the primary source for carbs in an animal's diet?

Grains

Why is fiber not considered to be a nutrient?

It does not provide calories

It cannot be digested for nutrients

Lipids are made up of which of the following building blocks?

Fatty acids

Which of the following are water soluble vitamins?

B, C

Why are fat soluble vitamins less critical compared to water soluble vitamins?

Fat soluble vitamins can be stored in the body for later use as needed.

Which mineral is essential for binding with hemoglobin in RBCs for oxygen transport?

Iron

Why should you NOT feed alfalfa hay in large amounts to guinea pigs?

High calcium, low Mg content

Ground feed, or 'meal', has which of the following biosafety concerns?

Respiratory due to dust

Which form of feed is ground into meal and molded into different shapes and sizes?

Pelleted feed

Pelleted feed is appropriate for rodents and rabbits because:

It helps maintain their teeth to prevent overgrowth.

Which form of feed is created using heat and pressure to form into different shapes?

Extruded feed

Which of the following forms of feed will you need to supplement with calcium?

Live

Which feed analysis would you choose if you were studying diet in your experiment?

Purified diet analysis

Autoclaving a diet that is NOT labeled for that purpose causes which of the following?

Nutrient deficiency

Why should you NOT feed a diet if the feed is beyond its expiration date?

No guarantee of nutrient quality or control

What is this item and what is it used for?

Nestlet; nesting activity and enrichment

Which of the following in NOT considered an emergency situation?

Handling soiled animal bedding using appropriate PPE and SOPs.

Which of the following ID methods is considered temporary?

Shaving fur

Which of the following ID methods is considered permanent?

Tattoo

Canned and semi-moist feeds are not appropriate for routine feeding for animals because:

It tends to stick to the teeth and gums compromising dental health.

True or False: Animal escapes are always an emergency!

False

Which activity is not considered a component of laboratory animal science?

Diagnosis and treatment of animal diseases

Who is responsible for planning and coordinating all phases of the research work on a research team?

Primary Investigator

Which of the following would include guidelines for care and use of livestock and poultry being used in agricultural research?

The Ag Guide

A written document that must be reviewed by the IACUC and that describes why animals are needed, what procedures will be performed on the animals, and how the animals will be housed and cared for throughout the project is called a(n):

Animal use protocol

What are roles that veterinary technicians may play on a research team?

Alert veterinary staff to ensure proper veterinary care to animals.

Immediately report environmental changes, changes in animal handling methods and error made in day to day husbandry tasks.

Control undesirable variable (differences in cleaning, sanitization procedures, feed and bedding, humidity, heat, light, noise)

Which of the following animals would not be covered under the Animal Welfare Act?

Poultry used on a poultry nutrition study (nutritional study)

Which of the following is TRUE regarding the members which MUST be included on the IACUC Committee?

At least one member must be a nonscientist representing public interests

To what document or regulation would you refer if a question about the proper method of euthanizing a fish were asked of you?

AVMA Guidelines on Euthanasia

What federal law was enacted initially to protect pet owners from the theft of their animals by preventing the sale or use of animals which have been stolen?

Animal Welfare Act (AWA)

If you are in the facility when a major disaster or accident occurs, what should be first priority?

Protecting human life

Who should receive training for handling security issues in an animal facility?

Everyone involved with work at a facility

Which document contains information regarding potential disasters, evacuation, and emergency contacts?

Disaster Plane

What is the best way to measure the effectiveness of an institution-wide disaster response plan?

Periodic distaster drills

What is the MOST common type of injury in the workplace?

Slips and falls

If you receive a bite wound from an animal in a laboratory research facility, you should scrub for 15 minutes with soap and warm water. What should you do next?

Stop all activities and notify your supervisor.

What is the purpose of an occupational health and safety plan?

A plan which is designed to protect your health and safety in the workplace by addressing your hazards and risks associated with your work responsibilities.

Which form of feed requires calcium supplementation when used as a diet?

Live

What is a biosecurity plan?

Actions and facility designs which prevent vandalism and theft of research animals, agents, and materials.

What is a hazard?

A material or system with the potential to cause harm